BtMf-AlphaCoV/GD2012

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Minunacovirus; Miniopterus bat coronavirus HKU8

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1WHC0|A0A0U1WHC0_9ALPC Uncharacterized protein OS=BtMf-AlphaCoV/GD2012 OX=1503280 PE=4 SV=1
MM1 pKa = 7.68LFLFLVNVAFAANQDD16 pKa = 3.66CTGNGNLDD24 pKa = 3.41SMEE27 pKa = 4.41DD28 pKa = 3.53LKK30 pKa = 11.71LNLPPNVTNAYY41 pKa = 9.54VSGYY45 pKa = 10.63LPLPQQWNCSVSGTLGNPRR64 pKa = 11.84VYY66 pKa = 10.19TGAHH70 pKa = 5.33GVFVSYY76 pKa = 10.88YY77 pKa = 10.82DD78 pKa = 3.33SATDD82 pKa = 3.25IVVGVGSTLSNSSWGMYY99 pKa = 9.2FFQKK103 pKa = 10.32NSEE106 pKa = 3.48GRR108 pKa = 11.84AIVRR112 pKa = 11.84ICKK115 pKa = 6.99WTSFNTPNYY124 pKa = 8.57QPNVNTQGRR133 pKa = 11.84DD134 pKa = 3.4CLFNKK139 pKa = 9.57QFSFTFIHH147 pKa = 6.42NGNEE151 pKa = 3.69IVGASWAGDD160 pKa = 3.28KK161 pKa = 10.87VVLYY165 pKa = 8.07TRR167 pKa = 11.84RR168 pKa = 11.84GLTTFYY174 pKa = 11.33VPGAEE179 pKa = 4.03FWDD182 pKa = 4.19VVSIRR187 pKa = 11.84CKK189 pKa = 10.81NKK191 pKa = 9.59GACAHH196 pKa = 5.59QVITSQSTVIVSTNGRR212 pKa = 11.84GLISNYY218 pKa = 7.15TLCSNCSGFPQHH230 pKa = 6.0VFAVGPDD237 pKa = 3.63GSIPPNFEE245 pKa = 3.62FEE247 pKa = 3.78NWFYY251 pKa = 10.55LTNSSSPVSGKK262 pKa = 9.85FVSVQPLQLACLWPIPALLGNTLPVYY288 pKa = 10.92FNMSNNNANCNGFKK302 pKa = 10.56ADD304 pKa = 3.76GVVDD308 pKa = 3.76SLRR311 pKa = 11.84FALNFSDD318 pKa = 3.24EE319 pKa = 4.13RR320 pKa = 11.84AFARR324 pKa = 11.84EE325 pKa = 4.09GVISLEE331 pKa = 4.52TVGNTYY337 pKa = 11.14NFTCTNSSTPTDD349 pKa = 3.44GSSFVLPFGVIDD361 pKa = 3.42QAYY364 pKa = 7.73YY365 pKa = 11.32CFMTFYY371 pKa = 11.26LNRR374 pKa = 11.84TADD377 pKa = 3.37LKK379 pKa = 8.28TTVFAGMLPPVVRR392 pKa = 11.84EE393 pKa = 3.72FVVMRR398 pKa = 11.84NGDD401 pKa = 3.5FYY403 pKa = 11.84LNGFRR408 pKa = 11.84VFSVGQVVSARR419 pKa = 11.84FNISSNDD426 pKa = 3.22SRR428 pKa = 11.84DD429 pKa = 3.15FWTVAFANNVPVLVEE444 pKa = 4.0INSTNIQQLLYY455 pKa = 10.73CNSPLNTIKK464 pKa = 10.65CQQLRR469 pKa = 11.84FNLDD473 pKa = 3.01NGFYY477 pKa = 10.35SYY479 pKa = 11.18SPQVNHH485 pKa = 6.27EE486 pKa = 4.18LPRR489 pKa = 11.84TVVRR493 pKa = 11.84LPKK496 pKa = 10.52FMTHH500 pKa = 5.09SVVNVSVAVSYY511 pKa = 11.16DD512 pKa = 3.34VVNQTNLGLIAWRR525 pKa = 11.84LSFADD530 pKa = 4.02HH531 pKa = 6.51WYY533 pKa = 10.48GWASDD538 pKa = 3.5NRR540 pKa = 11.84TTVCVDD546 pKa = 3.49TSSFTVKK553 pKa = 10.51LFTGSFSYY561 pKa = 11.09GSVFVDD567 pKa = 3.77VLAGEE572 pKa = 4.81CPFTLLSLNDD582 pKa = 3.63YY583 pKa = 11.03LSFGSLCLSLVPNGGCAMSVVTRR606 pKa = 11.84GSYY609 pKa = 10.88GEE611 pKa = 4.08PQSIAPLYY619 pKa = 10.81VSFSDD624 pKa = 3.46GDD626 pKa = 3.81NVIGVPKK633 pKa = 9.8PPPPGVLDD641 pKa = 3.75MSDD644 pKa = 3.04VYY646 pKa = 11.87YY647 pKa = 10.61NVCTSYY653 pKa = 10.57TIYY656 pKa = 10.56GHH658 pKa = 5.65TGRR661 pKa = 11.84GVISKK666 pKa = 10.13GAVDD670 pKa = 4.41FVSGLFYY677 pKa = 10.61TSPAGDD683 pKa = 3.45LLAYY687 pKa = 9.79KK688 pKa = 10.47NSTTGEE694 pKa = 3.91VFMIQPCQFASQVAVIADD712 pKa = 4.6TIVGAASSSANSSIPFNNTVDD733 pKa = 3.47AGLFYY738 pKa = 10.96YY739 pKa = 10.54HH740 pKa = 7.0FNNVTDD746 pKa = 4.05VNGTKK751 pKa = 10.53CEE753 pKa = 4.15VPTLTYY759 pKa = 10.66GGLGICADD767 pKa = 3.9GKK769 pKa = 10.41IVNATRR775 pKa = 11.84RR776 pKa = 11.84YY777 pKa = 8.69AAPEE781 pKa = 3.83PVSPVITGNISVPMNFSFSVQVEE804 pKa = 4.35YY805 pKa = 10.52IQIMLKK811 pKa = 10.27PVTVDD816 pKa = 2.84CSVYY820 pKa = 10.32VCNGNPRR827 pKa = 11.84CLQLLSQYY835 pKa = 11.5ASACKK840 pKa = 9.77TIEE843 pKa = 3.88QALQLSARR851 pKa = 11.84LEE853 pKa = 4.32SVEE856 pKa = 4.18VNSMVAISDD865 pKa = 3.53TALNLGVISKK875 pKa = 10.46FDD877 pKa = 3.37NTFNLSNVLPASVGGKK893 pKa = 8.93SVIEE897 pKa = 4.3DD898 pKa = 3.72LLFDD902 pKa = 3.94KK903 pKa = 11.1VVTSGLGTVDD913 pKa = 3.3TDD915 pKa = 3.77YY916 pKa = 11.21KK917 pKa = 11.24ACAEE921 pKa = 4.05KK922 pKa = 9.97MANTIAEE929 pKa = 4.49AGCVQYY935 pKa = 11.5YY936 pKa = 9.78NGIMVLPGVVDD947 pKa = 3.64QSLLGQYY954 pKa = 7.73TAALTGAMVMGGITAAAAIPFSIAVQSRR982 pKa = 11.84LNYY985 pKa = 10.06LALQTDD991 pKa = 4.07VLQRR995 pKa = 11.84NQQILAQSFNSAMGNITNAFEE1016 pKa = 4.4NVGNAIEE1023 pKa = 4.12QTSQGLQTVAQALDD1037 pKa = 3.47KK1038 pKa = 11.0VQNAVNEE1045 pKa = 4.12QGNALSHH1052 pKa = 5.84LTKK1055 pKa = 10.55QLASNFQAISASIEE1069 pKa = 3.88DD1070 pKa = 4.01LYY1072 pKa = 11.8VRR1074 pKa = 11.84LGQVEE1079 pKa = 4.59ADD1081 pKa = 3.54QQVDD1085 pKa = 3.49RR1086 pKa = 11.84LITGRR1091 pKa = 11.84LAALNAFVSQQLTKK1105 pKa = 9.57YY1106 pKa = 8.06TDD1108 pKa = 3.16VRR1110 pKa = 11.84ASRR1113 pKa = 11.84QLAQEE1118 pKa = 4.75KK1119 pKa = 10.45INEE1122 pKa = 4.44CVKK1125 pKa = 10.63SQSFRR1130 pKa = 11.84YY1131 pKa = 8.66GFCGNGTHH1139 pKa = 5.87VFSVANAAPDD1149 pKa = 4.35GIMFFHH1155 pKa = 7.06SVLLPTAYY1163 pKa = 10.48AEE1165 pKa = 4.25VNAYY1169 pKa = 10.26AGLCVDD1175 pKa = 3.32YY1176 pKa = 10.6KK1177 pKa = 11.25GYY1179 pKa = 10.42VLRR1182 pKa = 11.84DD1183 pKa = 3.44PGNVLFQKK1191 pKa = 10.27PGNEE1195 pKa = 4.26TYY1197 pKa = 10.87LITPRR1202 pKa = 11.84RR1203 pKa = 11.84MFEE1206 pKa = 3.66PRR1208 pKa = 11.84VPQMSDD1214 pKa = 2.99FVQISGCNVSYY1225 pKa = 11.53VNVSSEE1231 pKa = 4.04QLPDD1235 pKa = 3.41IVPDD1239 pKa = 4.35FMDD1242 pKa = 3.64VNATIQDD1249 pKa = 3.82ILDD1252 pKa = 3.66KK1253 pKa = 10.5LQNYY1257 pKa = 7.61SKK1259 pKa = 10.75PDD1261 pKa = 3.56FGLDD1265 pKa = 3.12IFNATYY1271 pKa = 11.05LNLSAEE1277 pKa = 4.48INDD1280 pKa = 3.59LTNRR1284 pKa = 11.84SQQLQAITDD1293 pKa = 3.75EE1294 pKa = 4.24LRR1296 pKa = 11.84ATIANINGTLVDD1308 pKa = 4.67LEE1310 pKa = 4.09WLNRR1314 pKa = 11.84VEE1316 pKa = 5.97TYY1318 pKa = 10.4IKK1320 pKa = 9.3WPWYY1324 pKa = 7.96VWLAIAVALIVLIGLMLWCCLSTGCCGCCSCMASTLDD1361 pKa = 3.63FRR1363 pKa = 11.84GSRR1366 pKa = 11.84LQQYY1370 pKa = 7.6EE1371 pKa = 4.15VEE1373 pKa = 4.46KK1374 pKa = 11.13VHH1376 pKa = 6.56IQQ1378 pKa = 3.09

Molecular weight: 150.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1UZ20|A0A0U1UZ20_9ALPC 3C-like proteinase OS=BtMf-AlphaCoV/GD2012 OX=1503280 PE=3 SV=1
MM1 pKa = 7.65FLRR4 pKa = 11.84KK5 pKa = 7.87TRR7 pKa = 11.84TSKK10 pKa = 10.73SSSNRR15 pKa = 11.84LMLIWTLLVRR25 pKa = 11.84TNQRR29 pKa = 11.84SSANLRR35 pKa = 11.84KK36 pKa = 9.72RR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 7.23TPLLNSILQHH50 pKa = 6.2LCSHH54 pKa = 7.03HH55 pKa = 6.31LL56 pKa = 3.51

Molecular weight: 6.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

9464

56

6896

1183.0

130.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.333 ± 0.228

3.318 ± 0.681

5.378 ± 0.612

3.91 ± 0.372

5.811 ± 0.285

6.826 ± 0.359

1.828 ± 0.347

4.522 ± 0.482

5.178 ± 0.987

8.432 ± 0.919

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.976 ± 0.102

5.547 ± 0.737

4.068 ± 0.609

2.98 ± 0.901

3.73 ± 0.656

7.069 ± 0.731

6.097 ± 0.233

10.366 ± 0.741

1.205 ± 0.219

4.427 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski