Carsonella ruddii (strain PV)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Zymobacter group; Candidatus Carsonella; Candidatus Carsonella ruddii

Average proteome isoelectric point is 8.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 182 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q05FS9|Q05FS9_CARRP Chaperone protein GrpE OS=Carsonella ruddii (strain PV) OX=387662 GN=CRP_061 PE=3 SV=1
MM1 pKa = 7.4SKK3 pKa = 10.24IIGIDD8 pKa = 3.1LGTTNSCIAVLSNGKK23 pKa = 8.53PQVIEE28 pKa = 3.79NSEE31 pKa = 4.16GGRR34 pKa = 11.84TTPSVVGYY42 pKa = 8.89TEE44 pKa = 4.46DD45 pKa = 3.12NRR47 pKa = 11.84IIVGLPAKK55 pKa = 9.93RR56 pKa = 11.84QAITNPKK63 pKa = 7.39NTLYY67 pKa = 10.75AIKK70 pKa = 10.29RR71 pKa = 11.84LIGRR75 pKa = 11.84KK76 pKa = 9.15FKK78 pKa = 11.12DD79 pKa = 4.33DD80 pKa = 3.5IVQKK84 pKa = 9.98DD85 pKa = 3.58IKK87 pKa = 9.43MVPYY91 pKa = 10.35KK92 pKa = 10.44IISSEE97 pKa = 4.08NGDD100 pKa = 2.84AWVEE104 pKa = 4.19VKK106 pKa = 10.36DD107 pKa = 4.43KK108 pKa = 11.09KK109 pKa = 10.54LAPPQISAEE118 pKa = 4.04ILKK121 pKa = 10.5KK122 pKa = 10.33MKK124 pKa = 8.91ITAEE128 pKa = 3.74NFLNEE133 pKa = 4.34KK134 pKa = 7.18VTKK137 pKa = 10.52AVITVPAYY145 pKa = 10.64FNDD148 pKa = 3.76SQRR151 pKa = 11.84QATKK155 pKa = 10.65DD156 pKa = 3.08AGKK159 pKa = 10.12IAGLEE164 pKa = 3.77VLRR167 pKa = 11.84IINEE171 pKa = 3.8PTAAALAYY179 pKa = 10.48GLDD182 pKa = 3.31KK183 pKa = 10.93KK184 pKa = 11.32KK185 pKa = 10.42NDD187 pKa = 4.34RR188 pKa = 11.84IIAVYY193 pKa = 10.39DD194 pKa = 3.67LGGGTFDD201 pKa = 4.02ISIIEE206 pKa = 4.03IANVDD211 pKa = 3.71GEE213 pKa = 4.74TQFEE217 pKa = 4.31VLSTNGDD224 pKa = 3.61TFLGGEE230 pKa = 4.53DD231 pKa = 3.37FDD233 pKa = 5.0IRR235 pKa = 11.84IINNLIYY242 pKa = 10.23EE243 pKa = 4.32FKK245 pKa = 10.47IEE247 pKa = 4.07NGINLSGDD255 pKa = 3.57SLAMQRR261 pKa = 11.84LKK263 pKa = 10.8EE264 pKa = 3.98AAEE267 pKa = 3.95KK268 pKa = 10.88AKK270 pKa = 10.53IEE272 pKa = 4.1LSSVEE277 pKa = 3.85QTDD280 pKa = 2.98INLPYY285 pKa = 9.34ITADD289 pKa = 3.2KK290 pKa = 10.96NGPKK294 pKa = 9.93HH295 pKa = 6.49LNIKK299 pKa = 8.1ITRR302 pKa = 11.84SKK304 pKa = 11.05LEE306 pKa = 3.96SLVEE310 pKa = 3.86DD311 pKa = 5.76LILKK315 pKa = 8.96SLKK318 pKa = 9.89PCEE321 pKa = 3.96IALNDD326 pKa = 4.18AKK328 pKa = 10.6ISKK331 pKa = 10.29NKK333 pKa = 8.79IDD335 pKa = 4.33EE336 pKa = 4.83IILVGGQTRR345 pKa = 11.84MPLVQKK351 pKa = 9.29MVSDD355 pKa = 4.12FFEE358 pKa = 4.45KK359 pKa = 10.62VVKK362 pKa = 10.48KK363 pKa = 10.61DD364 pKa = 3.42INPDD368 pKa = 3.02EE369 pKa = 4.32AVAIGASVQAGVLSGVVKK387 pKa = 10.69DD388 pKa = 4.1VLLLDD393 pKa = 3.83VTPLTLGIEE402 pKa = 4.34TMGGIMTPLIEE413 pKa = 4.93KK414 pKa = 8.43NTTIPTKK421 pKa = 9.18KK422 pKa = 7.9TQVFSTAEE430 pKa = 4.01DD431 pKa = 3.57NQTSVTIHH439 pKa = 5.96TLQGEE444 pKa = 4.46RR445 pKa = 11.84KK446 pKa = 9.26KK447 pKa = 10.88ALQNKK452 pKa = 9.55SLGKK456 pKa = 10.07FDD458 pKa = 5.67LNNISPAPRR467 pKa = 11.84GVPQIEE473 pKa = 4.08VSFDD477 pKa = 3.25LDD479 pKa = 3.65ANGILNVTAKK489 pKa = 10.49DD490 pKa = 3.16KK491 pKa = 10.47KK492 pKa = 9.18TGVEE496 pKa = 3.56QSIVIKK502 pKa = 10.81SSGGLSEE509 pKa = 5.73LEE511 pKa = 3.93IEE513 pKa = 5.0NMIKK517 pKa = 10.47DD518 pKa = 3.68AEE520 pKa = 4.39ANLEE524 pKa = 3.78IDD526 pKa = 3.88KK527 pKa = 11.08KK528 pKa = 11.0FEE530 pKa = 4.0EE531 pKa = 4.44LVKK534 pKa = 10.7CRR536 pKa = 11.84NEE538 pKa = 3.82ADD540 pKa = 3.56STISIVKK547 pKa = 10.26KK548 pKa = 10.29KK549 pKa = 10.99LKK551 pKa = 10.59DD552 pKa = 3.63EE553 pKa = 4.05NLKK556 pKa = 10.42ILDD559 pKa = 3.9EE560 pKa = 4.24EE561 pKa = 4.26RR562 pKa = 11.84VSIEE566 pKa = 3.66KK567 pKa = 10.58SISNLEE573 pKa = 3.99LLIKK577 pKa = 10.64GDD579 pKa = 5.22DD580 pKa = 3.24IDD582 pKa = 4.89SIKK585 pKa = 10.81KK586 pKa = 10.04EE587 pKa = 3.96NEE589 pKa = 3.76EE590 pKa = 4.27LLKK593 pKa = 11.25LSDD596 pKa = 4.31NIIKK600 pKa = 10.48KK601 pKa = 10.01KK602 pKa = 10.47

Molecular weight: 66.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q05FI1|RS7_CARRP 30S ribosomal protein S7 OS=Carsonella ruddii (strain PV) OX=387662 GN=rpsG PE=3 SV=1
MM1 pKa = 7.58TLNQILKK8 pKa = 9.49FKK10 pKa = 10.34RR11 pKa = 11.84KK12 pKa = 9.85KK13 pKa = 9.94SVKK16 pKa = 9.63KK17 pKa = 10.43KK18 pKa = 8.99KK19 pKa = 8.94TPALLSSPQKK29 pKa = 10.51KK30 pKa = 10.01GICIKK35 pKa = 10.84VYY37 pKa = 7.54TTTPKK42 pKa = 10.53KK43 pKa = 10.26PNSALRR49 pKa = 11.84KK50 pKa = 7.35VCRR53 pKa = 11.84VKK55 pKa = 10.92LSNKK59 pKa = 9.92NEE61 pKa = 3.43ITAYY65 pKa = 9.83IPGEE69 pKa = 3.86GHH71 pKa = 6.66NLQEE75 pKa = 4.3HH76 pKa = 6.37SNVLVRR82 pKa = 11.84GGRR85 pKa = 11.84VKK87 pKa = 10.82DD88 pKa = 3.7LPGVKK93 pKa = 9.6YY94 pKa = 10.19HH95 pKa = 7.61IIRR98 pKa = 11.84NVYY101 pKa = 9.84DD102 pKa = 3.63LSGVINRR109 pKa = 11.84KK110 pKa = 7.0TSRR113 pKa = 11.84SKK115 pKa = 10.87YY116 pKa = 9.16GKK118 pKa = 10.17KK119 pKa = 9.9KK120 pKa = 10.55

Molecular weight: 13.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

182

0

182

49923

37

1292

274.3

32.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

1.478 ± 0.135

1.633 ± 0.063

2.62 ± 0.115

3.327 ± 0.157

8.994 ± 0.251

3.698 ± 0.179

1.054 ± 0.049

15.522 ± 0.198

14.799 ± 0.35

10.134 ± 0.151

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.238 ± 0.057

11.776 ± 0.233

1.62 ± 0.08

1.476 ± 0.066

1.857 ± 0.09

6.344 ± 0.156

3.177 ± 0.102

3.219 ± 0.123

0.463 ± 0.049

5.573 ± 0.142
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski