Cardinium endosymbiont of Culicoides punctatus

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Amoebophilaceae; Candidatus Cardinium; unclassified Candidatus Cardinium

Average proteome isoelectric point is 7.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 917 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R5NZY3|A0A4R5NZY3_9BACT Fe(3+) dicitrate transport ATP-binding protein FecE OS=Cardinium endosymbiont of Culicoides punctatus OX=2304601 GN=fecE PE=4 SV=1
MM1 pKa = 7.23YY2 pKa = 7.73WTLEE6 pKa = 4.06LVSYY10 pKa = 11.06LEE12 pKa = 4.33DD13 pKa = 4.15APWPATKK20 pKa = 10.54DD21 pKa = 3.51EE22 pKa = 5.26LIDD25 pKa = 3.49YY26 pKa = 8.19AKK28 pKa = 10.6RR29 pKa = 11.84SGAPFEE35 pKa = 4.54VIRR38 pKa = 11.84NLEE41 pKa = 3.94EE42 pKa = 5.8LEE44 pKa = 5.08DD45 pKa = 4.4DD46 pKa = 3.66EE47 pKa = 5.87CLYY50 pKa = 11.02TSIEE54 pKa = 4.95DD55 pKa = 3.26IWPDD59 pKa = 3.78YY60 pKa = 8.43PTGDD64 pKa = 3.45DD65 pKa = 4.39FIFNEE70 pKa = 4.66DD71 pKa = 3.24EE72 pKa = 4.26YY73 pKa = 11.92

Molecular weight: 8.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R5P2G2|A0A4R5P2G2_9BACT Uncharacterized protein OS=Cardinium endosymbiont of Culicoides punctatus OX=2304601 GN=CCPUN_05260 PE=4 SV=1
MM1 pKa = 7.59EE2 pKa = 5.19LHH4 pKa = 5.89TLKK7 pKa = 10.59PAIGSLKK14 pKa = 9.25NKK16 pKa = 9.43KK17 pKa = 9.71RR18 pKa = 11.84VGRR21 pKa = 11.84GQGSGKK27 pKa = 10.57GGTATRR33 pKa = 11.84GYY35 pKa = 10.75NGAQSRR41 pKa = 11.84SGYY44 pKa = 8.61KK45 pKa = 9.93RR46 pKa = 11.84KK47 pKa = 9.86IGFEE51 pKa = 4.17GGQQPLQRR59 pKa = 11.84RR60 pKa = 11.84LPMYY64 pKa = 10.19GFKK67 pKa = 10.54CPSRR71 pKa = 11.84IEE73 pKa = 3.99FTPLNLNVLQALAEE87 pKa = 4.1KK88 pKa = 10.37HH89 pKa = 5.65NVSSIDD95 pKa = 3.24AHH97 pKa = 6.26FLRR100 pKa = 11.84QHH102 pKa = 5.94RR103 pKa = 11.84VIGKK107 pKa = 8.01NGKK110 pKa = 9.52YY111 pKa = 9.92KK112 pKa = 10.55ILGGGQLNIKK122 pKa = 10.15LSVAAHH128 pKa = 6.38RR129 pKa = 11.84CSASAMQAIQNLGGTVIILPIYY151 pKa = 9.05EE152 pKa = 4.02

Molecular weight: 16.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

917

0

917

302800

29

1488

330.2

37.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.307 ± 0.08

1.322 ± 0.033

4.886 ± 0.055

6.04 ± 0.085

4.448 ± 0.062

5.58 ± 0.061

2.66 ± 0.041

8.745 ± 0.073

7.864 ± 0.087

10.203 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.031

5.25 ± 0.063

3.541 ± 0.038

4.024 ± 0.052

3.898 ± 0.048

6.553 ± 0.057

5.708 ± 0.059

5.789 ± 0.057

0.902 ± 0.029

4.005 ± 0.056
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski