Streptomyces phage mu1/6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.32

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1WDG0|Q1WDG0_9CAUD Uncharacterized protein OS=Streptomyces phage mu1/6 OX=370623 PE=4 SV=1
MM1 pKa = 7.25STPTDD6 pKa = 3.53PTDD9 pKa = 4.28PGTGTPTDD17 pKa = 4.3PPPTGPVSEE26 pKa = 4.61PTPDD30 pKa = 3.23GQTWYY35 pKa = 9.21PQALVEE41 pKa = 4.4QQGWWHH47 pKa = 6.82PGDD50 pKa = 3.95PAYY53 pKa = 10.13MDD55 pKa = 3.97STWPAWQLPAWYY67 pKa = 9.27DD68 pKa = 3.33GRR70 pKa = 11.84TGAVAIPGTQGMPGTSQYY88 pKa = 10.67VWGGWLPWQAGSEE101 pKa = 4.27RR102 pKa = 11.84YY103 pKa = 9.28DD104 pKa = 4.9AATQWPAAVQRR115 pKa = 11.84TIHH118 pKa = 6.9DD119 pKa = 3.62MQAALGLLPPAPPAPADD136 pKa = 3.86PPQQPTDD143 pKa = 3.92SGPATPPITPPTLGQQPAEE162 pKa = 4.08TDD164 pKa = 3.56PEE166 pKa = 4.35GSII169 pKa = 4.64

Molecular weight: 17.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q1WDJ7|Q1WDJ7_9CAUD Uncharacterized protein OS=Streptomyces phage mu1/6 OX=370623 PE=4 SV=1
MM1 pKa = 7.79AKK3 pKa = 8.95TLQVGARR10 pKa = 11.84KK11 pKa = 9.28RR12 pKa = 11.84VGVSTDD18 pKa = 3.39DD19 pKa = 4.99LADD22 pKa = 3.33QVQQHH27 pKa = 5.73RR28 pKa = 11.84WDD30 pKa = 3.08VGARR34 pKa = 11.84IRR36 pKa = 11.84EE37 pKa = 4.01LRR39 pKa = 11.84LAAGLSQVDD48 pKa = 3.87LSHH51 pKa = 7.71RR52 pKa = 11.84IGVDD56 pKa = 2.5HH57 pKa = 6.39RR58 pKa = 11.84TISRR62 pKa = 11.84AEE64 pKa = 4.12NGVHH68 pKa = 7.51AISIDD73 pKa = 3.3QAYY76 pKa = 10.32RR77 pKa = 11.84IATALGQPSWRR88 pKa = 11.84LFRR91 pKa = 11.84EE92 pKa = 4.11PP93 pKa = 4.06

Molecular weight: 10.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

11547

49

1161

222.1

23.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.194 ± 0.701

0.849 ± 0.142

6.227 ± 0.242

4.91 ± 0.325

1.81 ± 0.134

8.608 ± 0.216

2.2 ± 0.223

3.958 ± 0.163

2.027 ± 0.218

8.799 ± 0.456

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.001 ± 0.139

2.061 ± 0.17

6.911 ± 0.462

4.798 ± 0.337

7.604 ± 0.395

4.633 ± 0.24

7.136 ± 0.417

7.223 ± 0.319

1.81 ± 0.199

2.243 ± 0.236
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski