Actinomyces urogenitalis DSM 15434

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; Actinomyces urogenitalis

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2394 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|C0W4F9|C0W4F9_9ACTO Uncharacterized protein OS=Actinomyces urogenitalis DSM 15434 OX=525246 GN=HMPREF0058_0753 PE=4 SV=1
MM1 pKa = 7.27HH2 pKa = 7.59AEE4 pKa = 3.77DD5 pKa = 5.72LYY7 pKa = 11.42YY8 pKa = 11.12LNAADD13 pKa = 3.58DD14 pKa = 3.62WARR17 pKa = 11.84RR18 pKa = 11.84DD19 pKa = 3.62EE20 pKa = 4.8IEE22 pKa = 5.51AEE24 pKa = 4.2DD25 pKa = 4.4AEE27 pKa = 4.81HH28 pKa = 6.88EE29 pKa = 3.98AAYY32 pKa = 9.64WDD34 pKa = 4.16DD35 pKa = 3.55ADD37 pKa = 4.84RR38 pKa = 11.84AYY40 pKa = 10.14EE41 pKa = 3.79QHH43 pKa = 6.98RR44 pKa = 11.84DD45 pKa = 3.47EE46 pKa = 5.83RR47 pKa = 11.84DD48 pKa = 2.89EE49 pKa = 4.97DD50 pKa = 5.15LYY52 pKa = 11.69DD53 pKa = 3.95NDD55 pKa = 4.41PYY57 pKa = 11.36EE58 pKa = 4.98GGEE61 pKa = 3.95AAA63 pKa = 5.36

Molecular weight:
7.48 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|C0W4T8|C0W4T8_9ACTO CobQ/CobB/MinD/ParA nucleotide binding domain protein OS=Actinomyces urogenitalis DSM 15434 OX=525246 GN=HMPREF0058_0882 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.24VHH17 pKa = 5.37GFRR20 pKa = 11.84TRR22 pKa = 11.84MSTRR26 pKa = 11.84AGRR29 pKa = 11.84AVLASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.61GRR40 pKa = 11.84ARR42 pKa = 11.84LAAA45 pKa = 4.44

Molecular weight:
5.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2394

0

2394

768598

32

1906

321.1

34.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.387 ± 0.067

0.779 ± 0.014

5.719 ± 0.042

5.932 ± 0.054

2.561 ± 0.036

8.907 ± 0.042

2.102 ± 0.027

3.729 ± 0.038

2.041 ± 0.042

10.222 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.066 ± 0.021

1.793 ± 0.028

5.414 ± 0.04

3.467 ± 0.032

7.154 ± 0.06

5.935 ± 0.037

6.257 ± 0.041

8.925 ± 0.048

1.553 ± 0.028

2.058 ± 0.029

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski