BtMr-AlphaCoV/SAX2011

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Myotacovirus; Myotis ricketti alphacoronavirus Sax-2011

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U1WHE1|A0A0U1WHE1_9ALPC Envelope small membrane protein OS=BtMr-AlphaCoV/SAX2011 OX=1503289 GN=E PE=3 SV=1
MM1 pKa = 6.69WHH3 pKa = 6.48VIVALVFIPFLSAANTGTCSNTINRR28 pKa = 11.84FMYY31 pKa = 10.38LKK33 pKa = 10.62LGLPVPTNDD42 pKa = 4.41AYY44 pKa = 10.35VTGYY48 pKa = 11.02LPIPSNWTCSDD59 pKa = 4.21GYY61 pKa = 9.12WQYY64 pKa = 12.08KK65 pKa = 7.89NAHH68 pKa = 5.89GLFVSYY74 pKa = 10.64HH75 pKa = 4.55STALDD80 pKa = 3.41FAIGVSSSTSYY91 pKa = 10.68AEE93 pKa = 3.66KK94 pKa = 9.31WGLYY98 pKa = 8.7FWQKK102 pKa = 10.31NNEE105 pKa = 3.48GRR107 pKa = 11.84AVFRR111 pKa = 11.84ICKK114 pKa = 8.79WDD116 pKa = 3.68ASAQTNFAPDD126 pKa = 2.69RR127 pKa = 11.84HH128 pKa = 5.12TARR131 pKa = 11.84DD132 pKa = 3.77CFVNKK137 pKa = 9.76QIPMVFKK144 pKa = 10.4HH145 pKa = 5.73QGNEE149 pKa = 3.64IVGVSWSGSRR159 pKa = 11.84VTLHH163 pKa = 5.65TLDD166 pKa = 3.7RR167 pKa = 11.84VFSFIVEE174 pKa = 4.39GAEE177 pKa = 3.75SWDD180 pKa = 3.79YY181 pKa = 11.12ATLRR185 pKa = 11.84CANGYY190 pKa = 9.72ACAHH194 pKa = 4.46QVVYY198 pKa = 11.2NPVTVIVTTVAAGIQKK214 pKa = 8.21YY215 pKa = 6.43TLCDD219 pKa = 3.16NCTGFPQHH227 pKa = 6.21VFATMEE233 pKa = 4.2NGEE236 pKa = 4.64IPPSFNFANWFYY248 pKa = 9.91LTNSSSPVSSRR259 pKa = 11.84VVGLQPLLLTCLWPIPALLGTATDD283 pKa = 3.25ITFDD287 pKa = 3.69RR288 pKa = 11.84NGTSDD293 pKa = 3.36VRR295 pKa = 11.84CNGFASNEE303 pKa = 3.99TADD306 pKa = 3.97AMRR309 pKa = 11.84FSLNFTDD316 pKa = 3.72SAVFAKK322 pKa = 10.53EE323 pKa = 3.72GVITLKK329 pKa = 10.01TLSNTFKK336 pKa = 10.67FSCSNSSTYY345 pKa = 8.85QAPYY349 pKa = 9.92VIPFGHH355 pKa = 7.2IDD357 pKa = 3.31QPYY360 pKa = 7.7YY361 pKa = 11.08CFTTFYY367 pKa = 10.53INEE370 pKa = 4.32TAGTTTTSFVGMLPPVVRR388 pKa = 11.84EE389 pKa = 3.81FVITKK394 pKa = 8.85TGNVYY399 pKa = 10.87LNGYY403 pKa = 10.02RR404 pKa = 11.84IFTVDD409 pKa = 4.2DD410 pKa = 3.9VVSVNFNISSTDD422 pKa = 3.08HH423 pKa = 6.65RR424 pKa = 11.84DD425 pKa = 2.95FWTVAFVKK433 pKa = 9.4NTEE436 pKa = 3.87VMLDD440 pKa = 3.4IEE442 pKa = 4.38DD443 pKa = 4.27TYY445 pKa = 11.24IKK447 pKa = 10.42QLLYY451 pKa = 11.09CNTPLNVVKK460 pKa = 10.35CQQLKK465 pKa = 10.06FVLDD469 pKa = 4.07DD470 pKa = 3.68GFYY473 pKa = 10.29SYY475 pKa = 11.27SSPVDD480 pKa = 3.23EE481 pKa = 4.52VLPRR485 pKa = 11.84TIVRR489 pKa = 11.84LPRR492 pKa = 11.84LMTHH496 pKa = 6.24NFLNFTIFVSFYY508 pKa = 10.9FDD510 pKa = 3.93DD511 pKa = 5.11DD512 pKa = 3.61KK513 pKa = 11.39QARR516 pKa = 11.84PDD518 pKa = 3.35GGFYY522 pKa = 10.7EE523 pKa = 5.23CATCAPKK530 pKa = 10.45YY531 pKa = 10.09YY532 pKa = 10.72KK533 pKa = 10.56LAFVDD538 pKa = 4.16DD539 pKa = 3.74WTSAVSSNVTSICVNYY555 pKa = 10.79ASFTTRR561 pKa = 11.84LFTFYY566 pKa = 10.84AGTTAGVHH574 pKa = 6.28LGLEE578 pKa = 4.41TGTCPFSFDD587 pKa = 3.4TLNNYY592 pKa = 7.65LTFGSLCFSLVANGGCTMNIVTQGPYY618 pKa = 10.77GLPHH622 pKa = 7.24TIAVLYY628 pKa = 10.27VSYY631 pKa = 11.01TEE633 pKa = 4.24GDD635 pKa = 3.78NIIGVPLSNIPPLGVSDD652 pKa = 4.0MSQVYY657 pKa = 10.77LDD659 pKa = 3.37TCTTYY664 pKa = 10.4TIYY667 pKa = 11.16GMTGRR672 pKa = 11.84GVITRR677 pKa = 11.84SNNTFITGLYY687 pKa = 6.7YY688 pKa = 10.52TSNAGNLLAYY698 pKa = 9.62KK699 pKa = 10.43NSTTGVVYY707 pKa = 10.5NVYY710 pKa = 9.9PCQLSSQVAVISDD723 pKa = 4.98AIVGMASSTPNVSIDD738 pKa = 3.61FNVTVVADD746 pKa = 3.52NFYY749 pKa = 10.78YY750 pKa = 10.73LSNSAQPCDD759 pKa = 3.98QPVLTYY765 pKa = 10.79AGIGICSDD773 pKa = 3.38GSITNSTARR782 pKa = 11.84RR783 pKa = 11.84AAADD787 pKa = 3.49PVSPVISGNISVPTNFTFSVQVEE810 pKa = 4.48YY811 pKa = 10.39IQLMLKK817 pKa = 10.04PVTVDD822 pKa = 2.84CSVYY826 pKa = 10.32VCNGNPRR833 pKa = 11.84CLQLLAQYY841 pKa = 11.14ASACRR846 pKa = 11.84TIEE849 pKa = 3.99QALQLSARR857 pKa = 11.84LEE859 pKa = 4.32SVEE862 pKa = 4.2VNSMISISQEE872 pKa = 3.4ALALGVIDD880 pKa = 4.95NFKK883 pKa = 10.97HH884 pKa = 6.74DD885 pKa = 4.11FNLTNVLPASVGAKK899 pKa = 9.92SAVEE903 pKa = 4.48DD904 pKa = 3.82LLFDD908 pKa = 3.81KK909 pKa = 11.09VVTSGLGTVDD919 pKa = 4.34ADD921 pKa = 3.94YY922 pKa = 11.12KK923 pKa = 10.27EE924 pKa = 4.6CASRR928 pKa = 11.84TANTVAEE935 pKa = 4.59VGCVQYY941 pKa = 11.44YY942 pKa = 9.74NGIMVLPGVVDD953 pKa = 3.61QSLLAQYY960 pKa = 10.48SAALTGAMVFGGVTAGAAVPFSIAVQSRR988 pKa = 11.84LNYY991 pKa = 10.06LALQTDD997 pKa = 4.07VLQRR1001 pKa = 11.84NQQQLANSFNAAMGNITEE1019 pKa = 4.28AFGRR1023 pKa = 11.84VNDD1026 pKa = 5.09AIEE1029 pKa = 4.21QTSHH1033 pKa = 7.28AISTVAQALDD1043 pKa = 3.45KK1044 pKa = 11.12VQTVVNDD1051 pKa = 3.14QGLALSQLTKK1061 pKa = 10.65QLASNFQAISSSIEE1075 pKa = 3.69DD1076 pKa = 3.87LYY1078 pKa = 11.76NRR1080 pKa = 11.84LDD1082 pKa = 3.53RR1083 pKa = 11.84VEE1085 pKa = 5.91ADD1087 pKa = 3.61LQVDD1091 pKa = 3.85RR1092 pKa = 11.84LITGRR1097 pKa = 11.84LAALNAFVAQQLTKK1111 pKa = 9.35YY1112 pKa = 8.42TDD1114 pKa = 3.16VRR1116 pKa = 11.84ASRR1119 pKa = 11.84QLAQDD1124 pKa = 4.7KK1125 pKa = 10.87INEE1128 pKa = 4.46CVKK1131 pKa = 10.55SQSFRR1136 pKa = 11.84YY1137 pKa = 8.66GFCGNGTHH1145 pKa = 6.04VFSVVNAAPDD1155 pKa = 3.22GMMFFHH1161 pKa = 7.24SVLLPTAYY1169 pKa = 9.73MEE1171 pKa = 4.19VAAFSGLCVEE1181 pKa = 4.56GNGYY1185 pKa = 7.66VLRR1188 pKa = 11.84DD1189 pKa = 3.41TGNVLFEE1196 pKa = 4.9KK1197 pKa = 10.4NGQYY1201 pKa = 10.86LITARR1206 pKa = 11.84KK1207 pKa = 7.74MFEE1210 pKa = 3.7PRR1212 pKa = 11.84VPQTSDD1218 pKa = 2.95FVQITGCDD1226 pKa = 3.24VVYY1229 pKa = 11.09LNVTRR1234 pKa = 11.84DD1235 pKa = 3.69EE1236 pKa = 4.88LPTVIPDD1243 pKa = 3.98YY1244 pKa = 10.97IDD1246 pKa = 3.46VNSTVEE1252 pKa = 4.81DD1253 pKa = 3.67ILSKK1257 pKa = 10.79LPNRR1261 pKa = 11.84TTPEE1265 pKa = 3.49FDD1267 pKa = 4.23LDD1269 pKa = 3.82IFNATYY1275 pKa = 11.19LNLTGEE1281 pKa = 4.41IADD1284 pKa = 3.56LTARR1288 pKa = 11.84SEE1290 pKa = 4.34SLKK1293 pKa = 9.87NTTLEE1298 pKa = 4.05LKK1300 pKa = 10.58EE1301 pKa = 4.49LIANINATLVDD1312 pKa = 4.66LEE1314 pKa = 4.13WLNRR1318 pKa = 11.84VEE1320 pKa = 5.97TYY1322 pKa = 10.41IKK1324 pKa = 9.23WPWWVWLIIVLVLILFTCLMLFCCCSTGCCGIFSCMASSCGACCDD1369 pKa = 3.2IRR1371 pKa = 11.84GTKK1374 pKa = 8.05LQRR1377 pKa = 11.84YY1378 pKa = 5.97EE1379 pKa = 4.8AIEE1382 pKa = 4.12KK1383 pKa = 10.11VHH1385 pKa = 5.3VQQ1387 pKa = 3.06

Molecular weight: 152.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U1WHF1|A0A0U1WHF1_9ALPC Nucleoprotein OS=BtMr-AlphaCoV/SAX2011 OX=1503289 PE=4 SV=1
MM1 pKa = 7.5SSVKK5 pKa = 10.2FEE7 pKa = 3.95ASGRR11 pKa = 11.84TGRR14 pKa = 11.84TPLSFFAPLTVSSDD28 pKa = 3.05KK29 pKa = 11.16KK30 pKa = 10.3FWNVLPKK37 pKa = 10.7NAVPTGKK44 pKa = 10.51GNAKK48 pKa = 9.27QQVGYY53 pKa = 10.09WNEE56 pKa = 3.72QPRR59 pKa = 11.84WRR61 pKa = 11.84MQRR64 pKa = 11.84GEE66 pKa = 4.18RR67 pKa = 11.84VEE69 pKa = 4.45KK70 pKa = 9.52PSYY73 pKa = 7.31WHH75 pKa = 6.93FYY77 pKa = 10.45FLGTGPHH84 pKa = 6.6ADD86 pKa = 2.73AKK88 pKa = 10.22FRR90 pKa = 11.84EE91 pKa = 4.68RR92 pKa = 11.84IPGVVWVSKK101 pKa = 11.16ANADD105 pKa = 4.36LNPTNLGTRR114 pKa = 11.84SKK116 pKa = 10.24ARR118 pKa = 11.84SVITPKK124 pKa = 10.17FDD126 pKa = 3.67SEE128 pKa = 4.47LPSDD132 pKa = 3.79IEE134 pKa = 4.32IVDD137 pKa = 3.81KK138 pKa = 11.41SSAPNSRR145 pKa = 11.84GNSRR149 pKa = 11.84SQSRR153 pKa = 11.84GPGNGANSRR162 pKa = 11.84NNSKK166 pKa = 10.14GRR168 pKa = 11.84NNTNSRR174 pKa = 11.84DD175 pKa = 3.47NSNNRR180 pKa = 11.84GKK182 pKa = 10.03SQSRR186 pKa = 11.84NSSRR190 pKa = 11.84NRR192 pKa = 11.84GQQRR196 pKa = 11.84NNNQQRR202 pKa = 11.84QGTGAAGNGTADD214 pKa = 3.77LAAAIVLALEE224 pKa = 4.28KK225 pKa = 10.8AGLTRR230 pKa = 11.84EE231 pKa = 4.24TEE233 pKa = 3.98KK234 pKa = 11.17APKK237 pKa = 9.88KK238 pKa = 10.39EE239 pKa = 4.0SANNNKK245 pKa = 9.64KK246 pKa = 10.22SEE248 pKa = 4.14SRR250 pKa = 11.84ASSPAPAQSKK260 pKa = 10.29KK261 pKa = 10.49DD262 pKa = 3.55QLGKK266 pKa = 10.98VMWKK270 pKa = 10.25RR271 pKa = 11.84NPDD274 pKa = 3.12PSFNVTQCFGPRR286 pKa = 11.84SVYY289 pKa = 10.72QNFGDD294 pKa = 3.81EE295 pKa = 4.18DD296 pKa = 4.5AIKK299 pKa = 10.87NGVKK303 pKa = 10.26AKK305 pKa = 10.39HH306 pKa = 5.33FPSWAEE312 pKa = 3.83LTPTPAALLFGSEE325 pKa = 4.5VITVEE330 pKa = 4.59DD331 pKa = 4.18GDD333 pKa = 5.63DD334 pKa = 3.88IVIQYY339 pKa = 9.04NYY341 pKa = 9.45QMRR344 pKa = 11.84VPKK347 pKa = 10.25TMAALQTFLPQVGAYY362 pKa = 10.47ANGDD366 pKa = 3.73NEE368 pKa = 4.72SEE370 pKa = 4.76SGDD373 pKa = 3.63QPGASAAAAAAPEE386 pKa = 4.52PPALPPKK393 pKa = 10.27LNPKK397 pKa = 9.94ADD399 pKa = 3.24AFVPPKK405 pKa = 10.38INPNYY410 pKa = 8.91FDD412 pKa = 3.54GMKK415 pKa = 10.3VEE417 pKa = 4.62IMNKK421 pKa = 9.31TISDD425 pKa = 3.85DD426 pKa = 3.6STAA429 pKa = 3.61

Molecular weight: 46.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

9000

76

6662

1500.0

166.65

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.378 ± 0.482

3.478 ± 0.785

5.656 ± 0.7

3.711 ± 0.364

5.511 ± 0.286

6.622 ± 0.453

1.856 ± 0.279

4.767 ± 0.863

5.2 ± 0.94

8.767 ± 0.829

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.178

5.678 ± 0.532

3.589 ± 0.643

3.056 ± 0.466

3.6 ± 0.351

6.956 ± 0.472

6.311 ± 0.912

9.644 ± 0.602

1.3 ± 0.427

4.822 ± 0.409
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski