Mycetocola reblochoni REB411

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Mycetocola; Mycetocola reblochoni

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2621 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R4I924|A0A1R4I924_9MICO Translation initiation factor IF-1 OS=Mycetocola reblochoni REB411 OX=1255698 GN=infA PE=3 SV=1
MM1 pKa = 7.33ARR3 pKa = 11.84IPEE6 pKa = 4.28PEE8 pKa = 4.0QTPEE12 pKa = 3.93GPAYY16 pKa = 9.98EE17 pKa = 4.59GRR19 pKa = 11.84LLDD22 pKa = 3.95RR23 pKa = 11.84ADD25 pKa = 4.13EE26 pKa = 4.22EE27 pKa = 5.19VVDD30 pKa = 4.29QGAPFDD36 pKa = 4.01IGTLMSRR43 pKa = 11.84RR44 pKa = 11.84GVLGLIGVGVGTAALAACSSTASTGGATSTATASATATATPSASPSATATAVLPDD99 pKa = 3.92GEE101 pKa = 5.03IPDD104 pKa = 4.17EE105 pKa = 4.32TAGPYY110 pKa = 10.2PGDD113 pKa = 3.77GSNGPDD119 pKa = 3.17VLEE122 pKa = 3.67QSGIVRR128 pKa = 11.84QDD130 pKa = 2.64IRR132 pKa = 11.84SSIGGGATADD142 pKa = 3.73GVPLTFTLTLTDD154 pKa = 4.03MASGDD159 pKa = 3.74APFEE163 pKa = 4.23GVAVYY168 pKa = 9.79AWHH171 pKa = 7.55CDD173 pKa = 3.19AQGRR177 pKa = 11.84YY178 pKa = 8.89SMYY181 pKa = 10.36SDD183 pKa = 5.31GITEE187 pKa = 3.9EE188 pKa = 4.3SYY190 pKa = 11.41LRR192 pKa = 11.84GVQVADD198 pKa = 3.6ADD200 pKa = 4.3GEE202 pKa = 4.79VTFTSVFPGCYY213 pKa = 7.56TGRR216 pKa = 11.84WTHH219 pKa = 5.38IHH221 pKa = 6.17FEE223 pKa = 4.24VYY225 pKa = 10.25PDD227 pKa = 3.59VEE229 pKa = 5.07SITDD233 pKa = 3.51ASNAIATSQMALPEE247 pKa = 4.38DD248 pKa = 3.97ACTAVYY254 pKa = 10.35ARR256 pKa = 11.84SEE258 pKa = 4.27YY259 pKa = 10.79SGSSQNLAQITLDD272 pKa = 3.42SDD274 pKa = 4.0NVFGDD279 pKa = 4.32DD280 pKa = 4.91GGALQLATVSGDD292 pKa = 3.25ADD294 pKa = 3.71SGYY297 pKa = 10.85LAALTVRR304 pKa = 11.84VDD306 pKa = 3.43TTTEE310 pKa = 3.94PTAGAAPDD318 pKa = 3.69GGGAGGPGGQPPSNN332 pKa = 3.52

Molecular weight: 33.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R4III0|A0A1R4III0_9MICO L-proline glycine betaine ABC transport system permease protein ProW (TC 3.A.1.12.1) OS=Mycetocola reblochoni REB411 OX=1255698 GN=FM119_01990 PE=3 SV=1
MM1 pKa = 7.21TRR3 pKa = 11.84TAATRR8 pKa = 11.84RR9 pKa = 11.84SGTTPRR15 pKa = 11.84RR16 pKa = 11.84LVLRR20 pKa = 11.84RR21 pKa = 11.84LAPPRR26 pKa = 11.84LTPPRR31 pKa = 11.84LVPSRR36 pKa = 11.84PSS38 pKa = 2.88

Molecular weight: 4.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2621

0

2621

853725

37

2761

325.7

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.736 ± 0.074

0.448 ± 0.009

6.351 ± 0.044

5.73 ± 0.049

2.782 ± 0.028

9.4 ± 0.053

1.858 ± 0.024

4.282 ± 0.038

1.617 ± 0.038

10.104 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.599 ± 0.02

1.859 ± 0.029

5.366 ± 0.038

2.64 ± 0.025

7.583 ± 0.063

6.101 ± 0.041

6.24 ± 0.048

9.129 ± 0.051

1.35 ± 0.021

1.823 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski