White clover mottle virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Polerovirus; unclassified Polerovirus

Average proteome isoelectric point is 7.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J1DTY0|A0A1J1DTY0_9LUTE Serine protease OS=White clover mottle virus OX=1913024 PE=4 SV=1
MM1 pKa = 7.41NFLINNQNSRR11 pKa = 11.84IEE13 pKa = 4.14LTFSNSLSLDD23 pKa = 3.27TRR25 pKa = 11.84RR26 pKa = 11.84SLTALFLISAEE37 pKa = 4.01QYY39 pKa = 10.4LQISHH44 pKa = 6.06EE45 pKa = 4.02QHH47 pKa = 5.34GTQNSVAYY55 pKa = 10.09CSLLVCSFSTRR66 pKa = 11.84GIQFPRR72 pKa = 11.84WQTCHH77 pKa = 6.61PLEE80 pKa = 4.54FEE82 pKa = 4.18KK83 pKa = 11.05SPPLRR88 pKa = 11.84ALPRR92 pKa = 11.84AFGEE96 pKa = 4.69GEE98 pKa = 4.0FCILSSSEE106 pKa = 3.57NGAAEE111 pKa = 4.64LYY113 pKa = 10.6SPNPNSCHH121 pKa = 6.36RR122 pKa = 11.84ANLYY126 pKa = 10.4RR127 pKa = 11.84LTYY130 pKa = 10.39SSLAEE135 pKa = 3.93GLPRR139 pKa = 11.84YY140 pKa = 9.58PEE142 pKa = 3.64IFGYY146 pKa = 8.14GTSFIQTLIGSYY158 pKa = 9.13IRR160 pKa = 11.84KK161 pKa = 8.97IEE163 pKa = 3.97RR164 pKa = 11.84GVFFLRR170 pKa = 11.84PVFPMGDD177 pKa = 3.29SLGVDD182 pKa = 3.65LRR184 pKa = 11.84TLGFLLLDD192 pKa = 3.28GRR194 pKa = 11.84GYY196 pKa = 10.63NNQYY200 pKa = 8.91HH201 pKa = 7.29AYY203 pKa = 7.89YY204 pKa = 9.97SSRR207 pKa = 11.84IANVLHH213 pKa = 7.03RR214 pKa = 11.84IYY216 pKa = 10.95GKK218 pKa = 10.49SFTLAFWRR226 pKa = 11.84LLQLDD231 pKa = 4.99CGPCFQVPSLPMEE244 pKa = 4.13APIAEE249 pKa = 4.64GTCGQDD255 pKa = 3.57SDD257 pKa = 6.07DD258 pKa = 4.42EE259 pKa = 5.76GEE261 pKa = 4.23DD262 pKa = 4.65DD263 pKa = 3.95EE264 pKa = 6.23GVWEE268 pKa = 4.21LL269 pKa = 4.43

Molecular weight: 30.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J1DP29|A0A1J1DP29_9LUTE p0 protein OS=White clover mottle virus OX=1913024 PE=4 SV=1
MM1 pKa = 6.53NTVVVRR7 pKa = 11.84NNGRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84NRR16 pKa = 11.84RR17 pKa = 11.84PIRR20 pKa = 11.84RR21 pKa = 11.84AQRR24 pKa = 11.84RR25 pKa = 11.84NPVVVVQPARR35 pKa = 11.84QPQRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84RR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84NRR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84AARR52 pKa = 11.84GSTAGRR58 pKa = 11.84RR59 pKa = 11.84GSSEE63 pKa = 3.79TFIFSKK69 pKa = 11.13DD70 pKa = 3.4NLKK73 pKa = 11.05GSDD76 pKa = 3.11SGRR79 pKa = 11.84ITFGPSLSDD88 pKa = 3.49CPAFSSGILKK98 pKa = 10.26AYY100 pKa = 10.13HH101 pKa = 6.54EE102 pKa = 4.61YY103 pKa = 10.53KK104 pKa = 9.31ITMVKK109 pKa = 10.64LEE111 pKa = 5.07FISEE115 pKa = 4.18AASTSSGSIAYY126 pKa = 9.43EE127 pKa = 3.84LDD129 pKa = 3.51PHH131 pKa = 6.89CKK133 pKa = 9.94ASEE136 pKa = 3.93LGSYY140 pKa = 9.5INKK143 pKa = 10.01FGITKK148 pKa = 10.23SGQRR152 pKa = 11.84TFSARR157 pKa = 11.84FINGIEE163 pKa = 3.94WHH165 pKa = 6.88SSDD168 pKa = 3.83EE169 pKa = 4.08DD170 pKa = 3.46QFRR173 pKa = 11.84ILYY176 pKa = 9.46KK177 pKa = 11.06GNGNSAIAGSFRR189 pKa = 11.84ITIKK193 pKa = 10.84CQTQNAKK200 pKa = 10.37

Molecular weight: 22.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

3296

193

1178

549.3

62.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.916 ± 0.193

1.881 ± 0.23

4.733 ± 0.396

6.796 ± 0.325

3.975 ± 0.308

6.493 ± 0.362

2.154 ± 0.37

5.036 ± 0.262

6.614 ± 0.81

8.677 ± 1.127

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.002 ± 0.216

5.036 ± 0.276

5.431 ± 0.408

3.914 ± 0.209

6.22 ± 1.104

8.799 ± 0.616

5.067 ± 0.499

5.674 ± 0.532

2.124 ± 0.251

3.428 ± 0.212
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski