Lactobacillus phage PL-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Junavirus; Lactobacillus virus PL1

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 59 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U5U408|U5U408_9CAUD Lysin OS=Lactobacillus phage PL-1 OX=39103 GN=PL1_23 PE=4 SV=1
MM1 pKa = 7.56KK2 pKa = 10.24LYY4 pKa = 10.93LVVCEE9 pKa = 4.94TGDD12 pKa = 3.44ADD14 pKa = 3.39QWEE17 pKa = 5.09GGTGEE22 pKa = 4.24ADD24 pKa = 3.04AVFATTDD31 pKa = 3.2KK32 pKa = 11.58AKK34 pKa = 10.8LDD36 pKa = 4.55DD37 pKa = 3.96YY38 pKa = 11.3LSTRR42 pKa = 11.84IHH44 pKa = 6.79GYY46 pKa = 10.92DD47 pKa = 2.94NVVTMEE53 pKa = 4.22LDD55 pKa = 3.3KK56 pKa = 10.9EE57 pKa = 4.35YY58 pKa = 11.3HH59 pKa = 6.55EE60 pKa = 4.75GTKK63 pKa = 9.93SAKK66 pKa = 10.08CLASWWEE73 pKa = 4.05EE74 pKa = 4.25GPCYY78 pKa = 9.71DD79 pKa = 5.57DD80 pKa = 5.52PMDD83 pKa = 3.25II84 pKa = 4.98

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U5U436|U5U436_9CAUD Uncharacterized protein OS=Lactobacillus phage PL-1 OX=39103 GN=PL1_54 PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 10.67SNVISLKK9 pKa = 10.26ILPEE13 pKa = 3.82YY14 pKa = 10.35FQAQVEE20 pKa = 4.63GKK22 pKa = 10.42KK23 pKa = 10.49NFEE26 pKa = 3.42IRR28 pKa = 11.84KK29 pKa = 8.96NDD31 pKa = 3.32RR32 pKa = 11.84EE33 pKa = 4.32YY34 pKa = 11.33KK35 pKa = 9.48VGSVLSLRR43 pKa = 11.84EE44 pKa = 3.85FDD46 pKa = 3.36GTKK49 pKa = 8.75YY50 pKa = 8.78TGRR53 pKa = 11.84RR54 pKa = 11.84AKK56 pKa = 10.76VIVTFITDD64 pKa = 3.29YY65 pKa = 10.44GQRR68 pKa = 11.84DD69 pKa = 4.43GYY71 pKa = 10.85VVLGTRR77 pKa = 11.84PFDD80 pKa = 3.29KK81 pKa = 10.91HH82 pKa = 5.78KK83 pKa = 10.3VTWEE87 pKa = 3.68EE88 pKa = 3.92KK89 pKa = 8.73RR90 pKa = 11.84WYY92 pKa = 9.55RR93 pKa = 11.84QYY95 pKa = 11.67FDD97 pKa = 3.51

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

59

0

59

13092

43

1620

221.9

24.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.662 ± 0.561

0.458 ± 0.093

7.386 ± 0.389

5.477 ± 0.314

3.43 ± 0.178

6.882 ± 0.376

1.742 ± 0.183

6.057 ± 0.278

7.401 ± 0.347

7.936 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.605 ± 0.158

4.95 ± 0.254

3.705 ± 0.215

4.354 ± 0.253

4.468 ± 0.306

6.622 ± 0.281

6.745 ± 0.388

5.973 ± 0.243

1.451 ± 0.123

3.697 ± 0.267
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski