Virgibacillus pantothenticus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Virgibacillus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3923 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0L0QM86|A0A0L0QM86_VIRPA Pyridoxal-5'-phosphate-dependent protein subunit beta OS=Virgibacillus pantothenticus OX=1473 GN=AFK71_14230 PE=3 SV=1
MM1 pKa = 7.69GYY3 pKa = 10.71DD4 pKa = 3.83LLIGTLINYY13 pKa = 7.67EE14 pKa = 4.25DD15 pKa = 4.24SDD17 pKa = 5.32MILEE21 pKa = 4.32WEE23 pKa = 4.06NGLRR27 pKa = 11.84IIGEE31 pKa = 4.24LDD33 pKa = 3.47TVFEE37 pKa = 4.47TDD39 pKa = 3.88NGLDD43 pKa = 3.73EE44 pKa = 6.27DD45 pKa = 4.52NINYY49 pKa = 8.85TEE51 pKa = 3.9YY52 pKa = 10.7DD53 pKa = 3.21AAVFKK58 pKa = 11.03VNKK61 pKa = 9.46ILSHH65 pKa = 6.8PSTNEE70 pKa = 3.17GSVYY74 pKa = 10.58NWLRR78 pKa = 11.84QEE80 pKa = 3.92KK81 pKa = 9.92SSLVEE86 pKa = 3.31ISLYY90 pKa = 10.65DD91 pKa = 3.94DD92 pKa = 4.55PPSAVYY98 pKa = 9.7LANGQCVWKK107 pKa = 10.29RR108 pKa = 11.84DD109 pKa = 3.63SS110 pKa = 3.41

Molecular weight: 12.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0L0QRV5|A0A0L0QRV5_VIRPA Phosphoesterase OS=Virgibacillus pantothenticus OX=1473 GN=AFK71_06550 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSTKK25 pKa = 10.07NGRR28 pKa = 11.84KK29 pKa = 8.84VIARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3923

0

3923

1123997

27

2001

286.5

32.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.183 ± 0.042

0.698 ± 0.012

5.075 ± 0.033

7.266 ± 0.05

4.531 ± 0.036

6.62 ± 0.038

2.2 ± 0.02

8.211 ± 0.043

6.931 ± 0.045

9.638 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.847 ± 0.019

4.461 ± 0.029

3.519 ± 0.021

4.224 ± 0.034

3.865 ± 0.028

5.67 ± 0.026

5.568 ± 0.023

6.823 ± 0.034

1.011 ± 0.015

3.657 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski