Nosema apis BRL 01

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Apansporoblastina; Nosematidae; Nosema; Nosema apis

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2727 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T0KW83|T0KW83_9MICR Retrotransposon-like family member (Retr-1) OS=Nosema apis BRL 01 OX=1037528 GN=NAPIS_ORF02686 PE=4 SV=1
MM1 pKa = 7.72SEE3 pKa = 4.28YY4 pKa = 10.97EE5 pKa = 4.19NNSDD9 pKa = 4.96EE10 pKa = 4.41NDD12 pKa = 3.54CSSSCEE18 pKa = 4.02EE19 pKa = 4.44SDD21 pKa = 3.46SGSDD25 pKa = 2.83IVVRR29 pKa = 11.84SHH31 pKa = 6.9RR32 pKa = 11.84VMQILSDD39 pKa = 4.68SYY41 pKa = 12.05DD42 pKa = 4.15DD43 pKa = 5.4DD44 pKa = 5.01DD45 pKa = 5.25NNYY48 pKa = 9.8FSDD51 pKa = 3.91TEE53 pKa = 4.25DD54 pKa = 3.73VPDD57 pKa = 4.78CEE59 pKa = 5.35SIDD62 pKa = 3.72VNLTQFTCLPPDD74 pKa = 3.48TQYY77 pKa = 11.76NKK79 pKa = 10.18QIPHH83 pKa = 5.74VFSEE87 pKa = 4.38LSGPKK92 pKa = 8.38HH93 pKa = 5.78TSPGDD98 pKa = 3.44AKK100 pKa = 10.85PIDD103 pKa = 4.02FSICFLLILLL113 pKa = 4.71

Molecular weight: 12.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T0KZ99|T0KZ99_9MICR Rna-binding protein OS=Nosema apis BRL 01 OX=1037528 GN=NAPIS_ORF01828 PE=4 SV=1
MM1 pKa = 7.06VAADD5 pKa = 4.49YY6 pKa = 10.8IRR8 pKa = 11.84EE9 pKa = 4.06IQKK12 pKa = 10.66AKK14 pKa = 10.34QSDD17 pKa = 2.79IMRR20 pKa = 11.84YY21 pKa = 8.39LLRR24 pKa = 11.84LRR26 pKa = 11.84VCEE29 pKa = 3.63YY30 pKa = 9.55RR31 pKa = 11.84QRR33 pKa = 11.84GEE35 pKa = 3.78VFRR38 pKa = 11.84VDD40 pKa = 3.27RR41 pKa = 11.84PTFIQRR47 pKa = 11.84ARR49 pKa = 11.84TLGYY53 pKa = 9.67RR54 pKa = 11.84SKK56 pKa = 11.12EE57 pKa = 3.98GILCYY62 pKa = 9.66IARR65 pKa = 11.84VKK67 pKa = 10.47KK68 pKa = 10.21GNRR71 pKa = 11.84KK72 pKa = 9.27RR73 pKa = 11.84EE74 pKa = 4.07YY75 pKa = 10.82NNGNTRR81 pKa = 11.84GKK83 pKa = 10.03CKK85 pKa = 9.95NARR88 pKa = 11.84IHH90 pKa = 6.52QITPSMRR97 pKa = 11.84HH98 pKa = 4.58QATAEE103 pKa = 3.93QIVGKK108 pKa = 9.4RR109 pKa = 11.84HH110 pKa = 5.48PNLRR114 pKa = 11.84VLNSYY119 pKa = 8.13WVNQDD124 pKa = 3.21LVYY127 pKa = 10.7KK128 pKa = 8.38YY129 pKa = 10.53FEE131 pKa = 4.68VIMVDD136 pKa = 3.57PFHH139 pKa = 6.7NAIRR143 pKa = 11.84NDD145 pKa = 3.44PKK147 pKa = 11.28LNWICKK153 pKa = 8.85GVMKK157 pKa = 10.5HH158 pKa = 5.47RR159 pKa = 11.84EE160 pKa = 4.16CRR162 pKa = 11.84GLTSASKK169 pKa = 10.02KK170 pKa = 10.39SRR172 pKa = 11.84GLGHH176 pKa = 7.19GIKK179 pKa = 10.6YY180 pKa = 10.51NNTIGGSVRR189 pKa = 11.84ACWKK193 pKa = 10.53RR194 pKa = 11.84NVTTSLRR201 pKa = 11.84RR202 pKa = 11.84FRR204 pKa = 5.05

Molecular weight: 24.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2727

0

2727

750327

52

1810

275.1

32.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.312 ± 0.044

2.01 ± 0.022

5.919 ± 0.038

7.015 ± 0.05

5.278 ± 0.047

3.509 ± 0.055

1.677 ± 0.019

10.337 ± 0.05

10.516 ± 0.057

9.283 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.029 ± 0.021

10.511 ± 0.102

2.177 ± 0.032

2.583 ± 0.029

3.125 ± 0.037

7.005 ± 0.059

4.724 ± 0.042

4.655 ± 0.037

0.531 ± 0.011

4.777 ± 0.039
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski