Flavobacterium phage Fpv1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Fipvunavirus; Flavobacterium virus Fpv1

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1B0WKI0|A0A1B0WKI0_9CAUD Uncharacterized protein OS=Flavobacterium phage Fpv1 OX=1792274 PE=4 SV=1
MM1 pKa = 7.11IHH3 pKa = 6.21SHH5 pKa = 5.1NTMQVFFSGTDD16 pKa = 3.29MAEE19 pKa = 4.27LNDD22 pKa = 4.02NSPSHH27 pKa = 6.32NFYY30 pKa = 11.11LSLIVNNYY38 pKa = 7.82MDD40 pKa = 4.94FMAKK44 pKa = 9.3IAFVASLEE52 pKa = 4.08QEE54 pKa = 3.97IKK56 pKa = 10.4EE57 pKa = 4.08IPYY60 pKa = 9.69YY61 pKa = 11.22ANDD64 pKa = 3.64EE65 pKa = 4.38NGVKK69 pKa = 10.53YY70 pKa = 10.58IIDD73 pKa = 3.83RR74 pKa = 11.84PNITLKK80 pKa = 9.86KK81 pKa = 10.36DD82 pKa = 2.86KK83 pKa = 10.54MFVYY87 pKa = 9.91DD88 pKa = 4.92CEE90 pKa = 4.5VISPFEE96 pKa = 3.77QVIVEE101 pKa = 4.16EE102 pKa = 4.88GFDD105 pKa = 3.64NRR107 pKa = 11.84VKK109 pKa = 10.72EE110 pKa = 3.97IMKK113 pKa = 9.16PKK115 pKa = 9.63PIKK118 pKa = 10.32QFTPHH123 pKa = 7.21TYY125 pKa = 9.84PATTNNTPVNTGFNNGKK142 pKa = 8.71WINGKK147 pKa = 8.93WIPNTPAIPAVNNLEE162 pKa = 4.03NAKK165 pKa = 9.85LANKK169 pKa = 9.17IKK171 pKa = 10.12HH172 pKa = 5.18YY173 pKa = 10.72KK174 pKa = 9.29IDD176 pKa = 3.57EE177 pKa = 4.47TKK179 pKa = 10.76LSNRR183 pKa = 11.84GKK185 pKa = 9.86KK186 pKa = 9.08VKK188 pKa = 10.48NIASMIDD195 pKa = 2.89IKK197 pKa = 10.41TFDD200 pKa = 4.91DD201 pKa = 4.14LSEE204 pKa = 5.24IDD206 pKa = 5.06DD207 pKa = 4.82LSPIDD212 pKa = 3.71TFTIEE217 pKa = 4.11LMKK220 pKa = 9.87FTTPIEE226 pKa = 4.29PNEE229 pKa = 4.12TLEE232 pKa = 5.36DD233 pKa = 3.98ALCLLEE239 pKa = 5.13DD240 pKa = 4.64LEE242 pKa = 4.39VDD244 pKa = 4.18AYY246 pKa = 10.91QIASSVIEE254 pKa = 4.64NYY256 pKa = 9.35TFLYY260 pKa = 10.41DD261 pKa = 3.67KK262 pKa = 10.52HH263 pKa = 7.75FPDD266 pKa = 3.99SADD269 pKa = 3.4EE270 pKa = 4.27EE271 pKa = 4.76FVEE274 pKa = 4.35DD275 pKa = 4.19TYY277 pKa = 12.05VVIDD281 pKa = 3.99LLGDD285 pKa = 4.11EE286 pKa = 4.98IMQFPFINVTIEE298 pKa = 4.26AIRR301 pKa = 11.84AMIVEE306 pKa = 4.33FEE308 pKa = 4.46KK309 pKa = 11.27NDD311 pKa = 3.56TTVV314 pKa = 2.47

Molecular weight: 36.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1B0WKV3|A0A1B0WKV3_9CAUD Uncharacterized protein OS=Flavobacterium phage Fpv1 OX=1792274 PE=4 SV=1
MM1 pKa = 7.45AVKK4 pKa = 10.37QFTYY8 pKa = 10.81LIGYY12 pKa = 9.35LGFIPQYY19 pKa = 8.31HH20 pKa = 6.77TIYY23 pKa = 10.25TPEE26 pKa = 3.87FYY28 pKa = 10.25IFEE31 pKa = 4.32KK32 pKa = 10.81EE33 pKa = 4.2KK34 pKa = 10.33IHH36 pKa = 6.61PKK38 pKa = 9.92HH39 pKa = 5.87KK40 pKa = 10.28QPRR43 pKa = 11.84PQQNLMLSNRR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84FFKK58 pKa = 10.12IHH60 pKa = 6.07KK61 pKa = 9.63LSRR64 pKa = 11.84KK65 pKa = 6.99DD66 pKa = 3.27TSYY69 pKa = 11.39KK70 pKa = 10.78GVGKK74 pKa = 10.23NYY76 pKa = 10.21KK77 pKa = 9.94

Molecular weight: 9.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

26991

74

4401

337.4

38.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.872 ± 0.27

0.911 ± 0.174

5.969 ± 0.158

7.747 ± 0.324

4.59 ± 0.163

5.494 ± 0.236

1.567 ± 0.074

8.006 ± 0.231

9.388 ± 0.319

8.277 ± 0.214

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.301 ± 0.121

7.732 ± 0.277

3.045 ± 0.146

3.853 ± 0.159

3.134 ± 0.18

6.106 ± 0.231

6.024 ± 0.186

5.517 ± 0.2

0.971 ± 0.104

4.487 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski