Candidatus Marinamargulisbacteria bacterium SCGC AG-439-L15

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Candidatus Margulisbacteria; Candidatus Marinamargulisbacteria

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 955 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A328RF04|A0A328RF04_9BACT Exodeoxyribonuclease III OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-439-L15 OX=2184346 GN=xth PE=3 SV=1
MM1 pKa = 7.51YY2 pKa = 10.44SGNCKK7 pKa = 9.68RR8 pKa = 11.84GYY10 pKa = 10.29IIGLMIAVVMVLSPLFSYY28 pKa = 10.68GALTPGVEE36 pKa = 3.99YY37 pKa = 10.32RR38 pKa = 11.84VVLEE42 pKa = 4.4RR43 pKa = 11.84LTSDD47 pKa = 3.42GEE49 pKa = 4.42GAIFTHH55 pKa = 5.44TTVTADD61 pKa = 3.04ANGIIEE67 pKa = 4.29YY68 pKa = 10.85AFDD71 pKa = 4.12NVPNCDD77 pKa = 3.58GADD80 pKa = 3.36SANFLIVSVIKK91 pKa = 10.55EE92 pKa = 4.4SDD94 pKa = 2.97WLGYY98 pKa = 9.68VPNSIVTSTVTFNVTEE114 pKa = 4.19EE115 pKa = 4.29SMSVVPAPPEE125 pKa = 3.91NTDD128 pKa = 3.06VFLGLNALSKK138 pKa = 10.65VQTKK142 pKa = 10.45GIIRR146 pKa = 11.84SFKK149 pKa = 9.28VTGKK153 pKa = 10.47ADD155 pKa = 3.73PLIALIGMVVFRR167 pKa = 11.84DD168 pKa = 3.73PDD170 pKa = 3.55LSDD173 pKa = 4.65SDD175 pKa = 3.64IQKK178 pKa = 10.09ISLGIADD185 pKa = 3.95QIFYY189 pKa = 10.15PGEE192 pKa = 3.74GFYY195 pKa = 11.1AQLSAVGVSDD205 pKa = 3.83EE206 pKa = 5.46KK207 pKa = 10.93IATFQKK213 pKa = 11.2ALVCEE218 pKa = 4.29SFGAGEE224 pKa = 4.1EE225 pKa = 4.39SFVGYY230 pKa = 8.9MRR232 pKa = 11.84EE233 pKa = 4.32TYY235 pKa = 10.49DD236 pKa = 3.57SNSVIMAGGDD246 pKa = 3.0EE247 pKa = 4.39GAARR251 pKa = 11.84EE252 pKa = 4.11AHH254 pKa = 5.91IKK256 pKa = 10.3AGEE259 pKa = 3.9RR260 pKa = 11.84LGAIVINAAEE270 pKa = 4.09YY271 pKa = 11.3AEE273 pKa = 4.81IDD275 pKa = 4.05MEE277 pKa = 4.67DD278 pKa = 3.75LLHH281 pKa = 6.89AVEE284 pKa = 5.3SLDD287 pKa = 5.42FEE289 pKa = 4.71DD290 pKa = 3.52TDD292 pKa = 4.65EE293 pKa = 4.2YY294 pKa = 11.65AFDD297 pKa = 3.74NRR299 pKa = 11.84IEE301 pKa = 4.0QLLSNSLSTFFQRR314 pKa = 11.84IYY316 pKa = 11.33VEE318 pKa = 4.59GTGSRR323 pKa = 11.84YY324 pKa = 8.28RR325 pKa = 11.84TALAGINASDD335 pKa = 3.65SQASRR340 pKa = 11.84FEE342 pKa = 4.68SAFSTLISSVEE353 pKa = 4.07EE354 pKa = 3.9LTDD357 pKa = 3.71PDD359 pKa = 3.51IYY361 pKa = 11.11EE362 pKa = 4.46FSDD365 pKa = 3.52SVAAFDD371 pKa = 5.54DD372 pKa = 4.43SRR374 pKa = 11.84DD375 pKa = 3.59SQYY378 pKa = 11.95DD379 pKa = 3.33SAQYY383 pKa = 10.42QFAVAIQSTEE393 pKa = 3.74AEE395 pKa = 4.19INALIDD401 pKa = 3.49TVADD405 pKa = 3.88IIGFDD410 pKa = 4.45DD411 pKa = 3.52PTSYY415 pKa = 11.0FNQNYY420 pKa = 9.98GSDD423 pKa = 3.49DD424 pKa = 3.42WGDD427 pKa = 3.28GRR429 pKa = 11.84TVSDD433 pKa = 3.83NANSHH438 pKa = 6.74WIFDD442 pKa = 4.25SLRR445 pKa = 11.84HH446 pKa = 6.76DD447 pKa = 3.7YY448 pKa = 9.64GTFFAEE454 pKa = 4.94IYY456 pKa = 10.49DD457 pKa = 4.2SGSGQYY463 pKa = 10.63NYY465 pKa = 10.41LRR467 pKa = 11.84ASIPQTVAFQYY478 pKa = 10.85VADD481 pKa = 4.32HH482 pKa = 6.19LQSGGTFSYY491 pKa = 9.52TWVDD495 pKa = 2.83IYY497 pKa = 11.28GGCDD501 pKa = 2.8TDD503 pKa = 4.37EE504 pKa = 4.62NVSSSDD510 pKa = 2.94ASMGMDD516 pKa = 4.51DD517 pKa = 3.27WWEE520 pKa = 4.47SNDD523 pKa = 3.32PTVWLWQLMDD533 pKa = 4.24LQWNVIDD540 pKa = 4.07LQRR543 pKa = 11.84WRR545 pKa = 11.84SDD547 pKa = 3.51YY548 pKa = 11.71YY549 pKa = 10.21NVQYY553 pKa = 11.08YY554 pKa = 10.35GADD557 pKa = 3.65DD558 pKa = 5.25FEE560 pKa = 4.58MTDD563 pKa = 2.83ITKK566 pKa = 10.42CYY568 pKa = 10.02STFLDD573 pKa = 3.69TMVTNINSDD582 pKa = 3.23MPAASKK588 pKa = 10.65KK589 pKa = 10.6YY590 pKa = 9.95ILNLFLRR597 pKa = 11.84PDD599 pKa = 3.41NNEE602 pKa = 4.13DD603 pKa = 3.62YY604 pKa = 11.38NPP606 pKa = 3.59

Molecular weight: 67.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A328RNM3|A0A328RNM3_9BACT Uncharacterized protein OS=Candidatus Marinamargulisbacteria bacterium SCGC AG-439-L15 OX=2184346 GN=DID77_03520 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.56RR3 pKa = 11.84TFQPNQRR10 pKa = 11.84KK11 pKa = 9.13RR12 pKa = 11.84KK13 pKa = 7.04KK14 pKa = 7.44THH16 pKa = 5.41GFLVRR21 pKa = 11.84MRR23 pKa = 11.84SVGGRR28 pKa = 11.84RR29 pKa = 11.84VIKK32 pKa = 9.82NRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.57GRR39 pKa = 11.84HH40 pKa = 3.82QLAAA44 pKa = 4.03

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

955

0

955

321027

29

6683

336.2

37.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.393 ± 0.093

1.135 ± 0.038

5.538 ± 0.069

6.271 ± 0.075

4.517 ± 0.061

6.335 ± 0.088

2.197 ± 0.035

7.041 ± 0.09

7.411 ± 0.09

10.598 ± 0.079

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.034

4.164 ± 0.053

4.004 ± 0.047

4.116 ± 0.068

3.966 ± 0.049

7.687 ± 0.085

5.952 ± 0.075

6.233 ± 0.076

0.841 ± 0.024

3.42 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski