Kordia sp. SMS9

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Kordia; unclassified Kordia

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4631 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345H667|A0A345H667_9FLAO Uncharacterized protein OS=Kordia sp. SMS9 OX=2282170 GN=KORDIASMS9_04317 PE=4 SV=1
MM1 pKa = 7.28NKK3 pKa = 10.02KK4 pKa = 10.26LLFVLLFLTGVTTNFAQGIFDD25 pKa = 4.85DD26 pKa = 5.16SIHH29 pKa = 6.12TATTFGSVNSPGGEE43 pKa = 3.47AVQNVIDD50 pKa = 3.97QNSNTKK56 pKa = 10.2FLDD59 pKa = 3.72FNAFDD64 pKa = 5.04GIGFEE69 pKa = 4.12VDD71 pKa = 2.7ILGVSSTAVAMEE83 pKa = 4.22FVTANDD89 pKa = 3.45APEE92 pKa = 4.7RR93 pKa = 11.84DD94 pKa = 3.57PTNYY98 pKa = 9.97EE99 pKa = 3.47ISGSNDD105 pKa = 2.43GVNFTSIATGTIPCVSTRR123 pKa = 11.84FFSRR127 pKa = 11.84TFSFTNTTSYY137 pKa = 10.39TSYY140 pKa = 10.97RR141 pKa = 11.84LNFTGTCGASSINQIADD158 pKa = 3.31VQLFEE163 pKa = 5.6SIGDD167 pKa = 3.96SPVITCPSNITVNNTTGQCDD187 pKa = 3.8AIVNYY192 pKa = 9.15TVTANDD198 pKa = 4.09TEE200 pKa = 5.54DD201 pKa = 4.68GMLTPNVTSGLPSGASFPVGITEE224 pKa = 4.04VFMNVTDD231 pKa = 3.85TDD233 pKa = 4.29NNTSSCSFTVTVVDD247 pKa = 4.53NEE249 pKa = 4.57DD250 pKa = 3.67PVAGCPANIMQTAANFGDD268 pKa = 3.57TTAVVNYY275 pKa = 8.32TLSPTDD281 pKa = 3.24NCVLINPLTDD291 pKa = 4.09FTPLTNINGKK301 pKa = 10.0AYY303 pKa = 10.02YY304 pKa = 10.25LSNNVFTPANAFLDD318 pKa = 4.91AISQGGFVGTIRR330 pKa = 11.84NASDD334 pKa = 2.94NTAITEE340 pKa = 5.02AIATAGGAADD350 pKa = 3.74VLIGFSDD357 pKa = 3.72TDD359 pKa = 3.68VEE361 pKa = 5.24GTFVWQSGDD370 pKa = 3.54ASTFDD375 pKa = 3.04NWNPGEE381 pKa = 4.15PNNAGNEE388 pKa = 4.33DD389 pKa = 3.83YY390 pKa = 10.93TVIQSSGGWNDD401 pKa = 2.79ITNGSSYY408 pKa = 10.9RR409 pKa = 11.84YY410 pKa = 9.74LLEE413 pKa = 3.74VDD415 pKa = 3.89YY416 pKa = 11.31APTQTAGLPSGADD429 pKa = 3.54FPIGTTTNTFTITDD443 pKa = 4.09LAGNSVMCSFDD454 pKa = 3.27VTVDD458 pKa = 3.41ANLSVAEE465 pKa = 4.1NSFSNRR471 pKa = 11.84IKK473 pKa = 10.2LTPNPTKK480 pKa = 10.91GLISFSNTANISLEE494 pKa = 4.32SIVIMDD500 pKa = 4.25LNGRR504 pKa = 11.84ILQTVMNIGQLSDD517 pKa = 3.94GEE519 pKa = 4.7TIDD522 pKa = 3.86ISNFAAGMYY531 pKa = 8.94FVRR534 pKa = 11.84LQDD537 pKa = 3.29IEE539 pKa = 4.78NNIAVKK545 pKa = 10.45RR546 pKa = 11.84IIKK549 pKa = 9.37QQ550 pKa = 3.13

Molecular weight: 58.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345H787|A0A345H787_9FLAO Transcriptional activator NphR OS=Kordia sp. SMS9 OX=2282170 GN=nphR PE=4 SV=1
MM1 pKa = 7.32FPLVSAILASLFRR14 pKa = 11.84IIFHH18 pKa = 6.58NSITTIFKK26 pKa = 7.68THH28 pKa = 6.9HH29 pKa = 5.95FRR31 pKa = 11.84MHH33 pKa = 6.39LNISKK38 pKa = 7.08THH40 pKa = 6.35RR41 pKa = 11.84FLAIFSTFCIALLLARR57 pKa = 11.84ILIKK61 pKa = 10.86GSMFYY66 pKa = 11.17VFLVWNLFLAIIPYY80 pKa = 10.15AISSWLLHH88 pKa = 5.27TRR90 pKa = 11.84WIRR93 pKa = 11.84KK94 pKa = 7.33HH95 pKa = 5.62TFPLCIFLGIWLLFLPNAPYY115 pKa = 10.85LITDD119 pKa = 4.39LMHH122 pKa = 6.81LRR124 pKa = 11.84YY125 pKa = 9.97AKK127 pKa = 10.58SSVIWFDD134 pKa = 3.3ALLLFAFALNGLLLGIFSLQHH155 pKa = 4.73VFQVLMEE162 pKa = 4.47RR163 pKa = 11.84WNVKK167 pKa = 4.23TAKK170 pKa = 10.19RR171 pKa = 11.84ILFCVVFLCGFGMYY185 pKa = 10.56LGRR188 pKa = 11.84FLRR191 pKa = 11.84WNSWEE196 pKa = 4.23LFSDD200 pKa = 4.1PMLLAQDD207 pKa = 4.45CFRR210 pKa = 11.84SILYY214 pKa = 8.6PAYY217 pKa = 10.49RR218 pKa = 11.84MKK220 pKa = 10.18TWGITLGFGSFLWVLLLGVQTFIPIKK246 pKa = 10.22KK247 pKa = 8.56ATTII251 pKa = 3.6

Molecular weight: 29.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4631

0

4631

1592471

29

5858

343.9

38.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.479 ± 0.035

0.848 ± 0.013

5.582 ± 0.049

6.568 ± 0.039

5.242 ± 0.032

5.961 ± 0.043

1.839 ± 0.017

7.938 ± 0.036

7.644 ± 0.059

8.991 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.091 ± 0.019

6.273 ± 0.046

3.268 ± 0.025

3.728 ± 0.026

3.385 ± 0.024

6.339 ± 0.029

6.545 ± 0.051

6.092 ± 0.029

1.002 ± 0.013

4.184 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski