Rhizobium phage B1VFA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G7NBW3|A0A7G7NBW3_9CAUD Uncharacterized protein OS=Rhizobium phage B1VFA OX=2759201 GN=B1VFA_050 PE=4 SV=1
MM1 pKa = 7.79SDD3 pKa = 3.5FLEE6 pKa = 4.31FMIILTSFCIVGMALVFAYY25 pKa = 9.59KK26 pKa = 10.52AIDD29 pKa = 3.48NYY31 pKa = 11.04GSNRR35 pKa = 11.84DD36 pKa = 2.84WWII39 pKa = 3.25

Molecular weight: 4.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G7NBT3|A0A7G7NBT3_9CAUD Uncharacterized protein OS=Rhizobium phage B1VFA OX=2759201 GN=B1VFA_027 PE=4 SV=1
MM1 pKa = 7.37SVQKK5 pKa = 10.38RR6 pKa = 11.84KK7 pKa = 10.17RR8 pKa = 11.84EE9 pKa = 3.95LLQLARR15 pKa = 11.84AICPKK20 pKa = 9.96ATIEE24 pKa = 4.26HH25 pKa = 6.63SGHH28 pKa = 6.51HH29 pKa = 5.33LQITIFGPNGSRR41 pKa = 11.84KK42 pKa = 9.28VFCASTAGDD51 pKa = 3.49HH52 pKa = 7.49RR53 pKa = 11.84DD54 pKa = 3.45TKK56 pKa = 10.57NVKK59 pKa = 9.82RR60 pKa = 11.84DD61 pKa = 3.37LLAAARR67 pKa = 11.84AIGLIANQNEE77 pKa = 4.24PPASQRR83 pKa = 3.35

Molecular weight: 9.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

160

0

160

36726

34

2064

229.5

25.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.761 ± 0.272

0.795 ± 0.064

6.431 ± 0.155

6.845 ± 0.28

3.847 ± 0.124

7.382 ± 0.247

1.89 ± 0.097

5.74 ± 0.096

5.982 ± 0.248

8.109 ± 0.168

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.679 ± 0.134

3.817 ± 0.162

4.22 ± 0.146

3.542 ± 0.15

5.821 ± 0.163

5.296 ± 0.176

6.031 ± 0.348

6.676 ± 0.182

1.315 ± 0.083

2.818 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski