Lactococcus phage BK5-T

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Sandinevirus; Lactococcus virus BK5T

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q94MA9|Q94MA9_9CAUD Major structural protein OS=Lactococcus phage BK5-T OX=31754 PE=2 SV=1
MM1 pKa = 7.25TVTVDD6 pKa = 4.86DD7 pKa = 5.45LLDD10 pKa = 3.62QLSEE14 pKa = 4.54DD15 pKa = 5.09DD16 pKa = 4.43DD17 pKa = 4.84RR18 pKa = 11.84KK19 pKa = 10.14PQLQIYY25 pKa = 9.63FDD27 pKa = 3.54TAKK30 pKa = 10.64AYY32 pKa = 10.25VKK34 pKa = 10.59NAVSSDD40 pKa = 3.45TVDD43 pKa = 3.3APFFSVEE50 pKa = 3.92NVYY53 pKa = 10.36PIYY56 pKa = 10.78DD57 pKa = 3.83VAVLSYY63 pKa = 11.85SMDD66 pKa = 2.86LWINRR71 pKa = 11.84STTMPPTTAVDD82 pKa = 3.39HH83 pKa = 6.19MVGQLRR89 pKa = 11.84GLYY92 pKa = 10.13SSWKK96 pKa = 9.14EE97 pKa = 3.89AQDD100 pKa = 3.7GQNLQTEE107 pKa = 4.54

Molecular weight: 12.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q94MB3|Q94MB3_9CAUD Putative large terminase subunit OS=Lactococcus phage BK5-T OX=31754 PE=2 SV=1
MM1 pKa = 7.02YY2 pKa = 10.91KK3 pKa = 10.87LMIFNQNLSRR13 pKa = 11.84NNFRR17 pKa = 11.84NFFSISYY24 pKa = 10.37LSDD27 pKa = 4.33FIKK30 pKa = 10.9NSSCKK35 pKa = 9.94VKK37 pKa = 10.86AIILTSFMRR46 pKa = 11.84NSTNFIIHH54 pKa = 6.6FLHH57 pKa = 7.6DD58 pKa = 3.39NFIIQNRR65 pKa = 11.84TISQTCFSSNCFKK78 pKa = 10.69WFNFYY83 pKa = 11.22NCLKK87 pKa = 10.41HH88 pKa = 6.62RR89 pKa = 11.84LNISKK94 pKa = 9.88QFACLFKK101 pKa = 10.77VYY103 pKa = 10.3RR104 pKa = 11.84GNN106 pKa = 3.41

Molecular weight: 12.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

12908

42

1904

211.6

23.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.477 ± 0.764

0.589 ± 0.152

5.95 ± 0.224

5.834 ± 0.485

3.99 ± 0.332

6.856 ± 0.741

1.371 ± 0.142

6.988 ± 0.412

8.669 ± 0.577

7.879 ± 0.539

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.533 ± 0.198

6.732 ± 0.318

3.153 ± 0.373

4.222 ± 0.447

3.223 ± 0.293

7.112 ± 0.31

7.468 ± 0.885

5.741 ± 0.224

1.379 ± 0.262

3.835 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski