Mycobacterium phage Breeniome

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Bixzunavirus; Mycobacterium virus Bxz1

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 236 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|T2FID2|T2FID2_9CAUD Uncharacterized protein OS=Mycobacterium phage Breeniome OX=1340712 GN=143 PE=4 SV=1
MM1 pKa = 7.81ADD3 pKa = 3.58YY4 pKa = 11.13ASDD7 pKa = 4.1CPISRR12 pKa = 11.84PVDD15 pKa = 3.83PGWTSDD21 pKa = 2.87WDD23 pKa = 3.89LGGMQWSYY31 pKa = 11.65DD32 pKa = 3.36YY33 pKa = 11.9GMFYY37 pKa = 10.44QPSPDD42 pKa = 3.22EE43 pKa = 4.38LLPLRR48 pKa = 11.84KK49 pKa = 8.64PAQRR53 pKa = 11.84VGEE56 pKa = 4.11

Molecular weight: 6.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|T2FHQ0|T2FHQ0_9CAUD Uncharacterized protein OS=Mycobacterium phage Breeniome OX=1340712 GN=26 PE=4 SV=1
MM1 pKa = 7.17QLRR4 pKa = 11.84IIRR7 pKa = 11.84GGAMSSDD14 pKa = 4.14FWWGMFVIPAAALAVAGVLVAVMAVIWASAKK45 pKa = 9.67WGGNEE50 pKa = 3.63YY51 pKa = 10.91KK52 pKa = 10.34LWPKK56 pKa = 10.29RR57 pKa = 11.84WGQRR61 pKa = 11.84EE62 pKa = 4.31SIVTVVATAKK72 pKa = 9.94SVRR75 pKa = 11.84YY76 pKa = 8.89LWIPGWHH83 pKa = 5.78IVICRR88 pKa = 11.84TTMATRR94 pKa = 11.84EE95 pKa = 4.3SRR97 pKa = 11.84PEE99 pKa = 3.32WQRR102 pKa = 11.84RR103 pKa = 11.84QRR105 pKa = 11.84VQHH108 pKa = 6.72AIGAAIRR115 pKa = 11.84AEE117 pKa = 4.23EE118 pKa = 4.07EE119 pKa = 3.92AA120 pKa = 4.55

Molecular weight: 13.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

236

0

236

47590

17

1370

201.7

22.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.742 ± 0.24

0.853 ± 0.073

7.025 ± 0.18

6.388 ± 0.176

3.078 ± 0.098

8.075 ± 0.268

2.482 ± 0.139

4.306 ± 0.14

3.805 ± 0.137

7.573 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.532 ± 0.1

3.379 ± 0.106

5.77 ± 0.171

4.114 ± 0.124

6.88 ± 0.179

5.493 ± 0.162

6.466 ± 0.188

7.134 ± 0.202

1.904 ± 0.087

3.003 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski