Rhodococcus pyridinivorans SB3094

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; Rhodococcus pyridinivorans

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4825 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V9XE53|V9XE53_9NOCA Cytochrome c oxidase polypeptide 4 OS=Rhodococcus pyridinivorans SB3094 OX=1435356 GN=Y013_05985 PE=3 SV=1
MM1 pKa = 8.11RR2 pKa = 11.84DD3 pKa = 3.51QLPPGLPPDD12 pKa = 4.75PFAGDD17 pKa = 3.84PDD19 pKa = 4.56DD20 pKa = 5.19PSAALDD26 pKa = 4.59AIEE29 pKa = 5.17PGQPLDD35 pKa = 3.45PAEE38 pKa = 4.11RR39 pKa = 11.84LAVEE43 pKa = 4.58EE44 pKa = 4.77DD45 pKa = 3.54LADD48 pKa = 3.98LAVYY52 pKa = 8.34EE53 pKa = 4.57ALLAPRR59 pKa = 11.84GVRR62 pKa = 11.84GLVVCCEE69 pKa = 4.61DD70 pKa = 3.47CHH72 pKa = 7.37QDD74 pKa = 4.69HH75 pKa = 6.05YY76 pKa = 11.39HH77 pKa = 6.58DD78 pKa = 3.93WDD80 pKa = 3.57MLRR83 pKa = 11.84ANLLQLLVDD92 pKa = 4.02GTVRR96 pKa = 11.84PHH98 pKa = 6.65EE99 pKa = 4.33PAFDD103 pKa = 4.51PSPDD107 pKa = 3.75AYY109 pKa = 10.08VTWDD113 pKa = 3.35YY114 pKa = 11.62CRR116 pKa = 11.84GYY118 pKa = 11.37ADD120 pKa = 3.97ASMNEE125 pKa = 4.27ALPVDD130 pKa = 4.7PYY132 pKa = 11.84GLDD135 pKa = 3.27SS136 pKa = 3.97

Molecular weight: 14.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V9XFD6|V9XFD6_9NOCA Acyl-CoA synthetase OS=Rhodococcus pyridinivorans SB3094 OX=1435356 GN=Y013_04705 PE=4 SV=1
MM1 pKa = 7.69AKK3 pKa = 10.06GKK5 pKa = 8.69RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84VHH19 pKa = 5.99GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVSARR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.72GRR42 pKa = 11.84ASLTAA47 pKa = 4.1

Molecular weight: 5.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4825

0

4825

1519572

29

9122

314.9

33.87

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.605 ± 0.049

0.74 ± 0.012

6.601 ± 0.036

5.94 ± 0.033

2.986 ± 0.02

8.819 ± 0.039

2.22 ± 0.017

4.048 ± 0.023

1.973 ± 0.035

9.967 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.859 ± 0.016

1.902 ± 0.02

5.709 ± 0.035

2.586 ± 0.021

7.68 ± 0.04

5.416 ± 0.024

6.307 ± 0.03

9.177 ± 0.039

1.444 ± 0.015

2.021 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski