Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) (Maize anthracnose fungus) (Glomerella graminicola)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Sordariomycetes; Hypocreomycetidae; Glomerellales; Glomerellaceae; Colletotrichum; Colletotrichum graminicola species complex; Colletotrichum graminicola

Average proteome isoelectric point is 6.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12019 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E3QHW1|E3QHW1_COLGM Major facilitator superfamily transporter OS=Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) OX=645133 GN=GLRG_05593 PE=4 SV=1
MM1 pKa = 7.75SLGEE5 pKa = 4.5CSNICKK11 pKa = 10.44ANPQCLSFQFTDD23 pKa = 3.46YY24 pKa = 11.41GRR26 pKa = 11.84GEE28 pKa = 4.1MACYY32 pKa = 9.36TFNLPAAAAIVPDD45 pKa = 4.34TANSPDD51 pKa = 4.18PEE53 pKa = 4.97PEE55 pKa = 3.69SDD57 pKa = 4.32ADD59 pKa = 3.68ADD61 pKa = 4.44LFNTNLDD68 pKa = 3.82TNLDD72 pKa = 3.63ADD74 pKa = 5.01LDD76 pKa = 4.24ADD78 pKa = 5.01LDD80 pKa = 4.32ANLDD84 pKa = 3.51ADD86 pKa = 4.32PNHH89 pKa = 6.82RR90 pKa = 11.84PSPNLWRR97 pKa = 11.84DD98 pKa = 3.25GLRR101 pKa = 3.57

Molecular weight: 10.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E3QD49|E3QD49_COLGM Uncharacterized protein OS=Colletotrichum graminicola (strain M1.001 / M2 / FGSC 10212) OX=645133 GN=GLRG_03965 PE=4 SV=1
MM1 pKa = 7.85PLRR4 pKa = 11.84STRR7 pKa = 11.84HH8 pKa = 3.77TTTAPRR14 pKa = 11.84RR15 pKa = 11.84NGLFSRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84AAPATTTATTRR34 pKa = 11.84TTHH37 pKa = 5.31TKK39 pKa = 10.34RR40 pKa = 11.84GGLFSRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84GPVHH52 pKa = 5.74TAAPVHH58 pKa = 4.94HH59 pKa = 6.95HH60 pKa = 5.97RR61 pKa = 11.84RR62 pKa = 11.84KK63 pKa = 9.97PSMGDD68 pKa = 3.3KK69 pKa = 10.53ISGAMLKK76 pKa = 10.72LKK78 pKa = 9.36GTLTRR83 pKa = 11.84RR84 pKa = 11.84PGQKK88 pKa = 9.9AAGTRR93 pKa = 11.84RR94 pKa = 11.84MHH96 pKa = 5.68GTDD99 pKa = 2.9GRR101 pKa = 11.84GSRR104 pKa = 11.84RR105 pKa = 11.84YY106 pKa = 9.57

Molecular weight: 11.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12019

0

12019

5585661

31

8935

464.7

51.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.286 ± 0.021

1.209 ± 0.008

5.78 ± 0.017

6.024 ± 0.024

3.696 ± 0.013

7.2 ± 0.023

2.31 ± 0.01

4.628 ± 0.015

4.775 ± 0.024

8.687 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.009

3.585 ± 0.013

6.172 ± 0.029

3.888 ± 0.017

6.119 ± 0.023

7.934 ± 0.026

5.915 ± 0.023

6.378 ± 0.017

1.487 ± 0.008

2.696 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski