Amino acid dipepetide frequency for Methanothermobacter tenebrarum

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.591AlaAla: 5.591 ± 0.149
0.92AlaCys: 0.92 ± 0.061
3.521AlaAsp: 3.521 ± 0.099
4.449AlaGlu: 4.449 ± 0.105
2.45AlaPhe: 2.45 ± 0.083
4.804AlaGly: 4.804 ± 0.117
1.283AlaHis: 1.283 ± 0.06
6.529AlaIle: 6.529 ± 0.139
4.921AlaLys: 4.921 ± 0.118
6.426AlaLeu: 6.426 ± 0.122
1.761AlaMet: 1.761 ± 0.072
2.214AlaAsn: 2.214 ± 0.084
2.322AlaPro: 2.322 ± 0.065
1.432AlaGln: 1.432 ± 0.065
3.273AlaArg: 3.273 ± 0.093
3.665AlaSer: 3.665 ± 0.099
3.413AlaThr: 3.413 ± 0.095
4.047AlaVal: 4.047 ± 0.107
0.451AlaTrp: 0.451 ± 0.035
2.294AlaTyr: 2.294 ± 0.077
0.0AlaXaa: 0.0 ± 0.0
Cys
0.739CysAla: 0.739 ± 0.049
0.162CysCys: 0.162 ± 0.025
0.615CysAsp: 0.615 ± 0.041
0.826CysGlu: 0.826 ± 0.046
0.341CysPhe: 0.341 ± 0.029
1.331CysGly: 1.331 ± 0.068
0.254CysHis: 0.254 ± 0.026
0.938CysIle: 0.938 ± 0.056
0.808CysLys: 0.808 ± 0.042
0.824CysLeu: 0.824 ± 0.038
0.275CysMet: 0.275 ± 0.025
0.512CysAsn: 0.512 ± 0.033
0.993CysPro: 0.993 ± 0.069
0.307CysGln: 0.307 ± 0.028
0.48CysArg: 0.48 ± 0.034
0.551CysSer: 0.551 ± 0.033
0.538CysThr: 0.538 ± 0.038
0.595CysVal: 0.595 ± 0.041
0.08CysTrp: 0.08 ± 0.015
0.407CysTyr: 0.407 ± 0.028
0.0CysXaa: 0.0 ± 0.0
Asp
3.303AspAla: 3.303 ± 0.091
0.515AspCys: 0.515 ± 0.03
2.871AspAsp: 2.871 ± 0.084
4.653AspGlu: 4.653 ± 0.108
2.37AspPhe: 2.37 ± 0.083
3.248AspGly: 3.248 ± 0.096
1.032AspHis: 1.032 ± 0.051
5.792AspIle: 5.792 ± 0.116
4.253AspLys: 4.253 ± 0.108
5.083AspLeu: 5.083 ± 0.117
1.311AspMet: 1.311 ± 0.057
1.944AspAsn: 1.944 ± 0.066
2.784AspPro: 2.784 ± 0.083
0.888AspGln: 0.888 ± 0.041
2.011AspArg: 2.011 ± 0.072
2.217AspSer: 2.217 ± 0.072
2.345AspThr: 2.345 ± 0.074
3.516AspVal: 3.516 ± 0.087
0.423AspTrp: 0.423 ± 0.033
2.276AspTyr: 2.276 ± 0.076
0.0AspXaa: 0.0 ± 0.0
Glu
5.604GluAla: 5.604 ± 0.118
0.885GluCys: 0.885 ± 0.045
5.319GluAsp: 5.319 ± 0.123
7.524GluGlu: 7.524 ± 0.174
2.713GluPhe: 2.713 ± 0.075
4.687GluGly: 4.687 ± 0.118
1.395GluHis: 1.395 ± 0.059
7.709GluIle: 7.709 ± 0.158
8.624GluLys: 8.624 ± 0.177
6.735GluLeu: 6.735 ± 0.127
1.96GluMet: 1.96 ± 0.077
4.202GluAsn: 4.202 ± 0.099
2.377GluPro: 2.377 ± 0.075
1.066GluGln: 1.066 ± 0.057
3.742GluArg: 3.742 ± 0.101
3.408GluSer: 3.408 ± 0.079
3.717GluThr: 3.717 ± 0.093
4.699GluVal: 4.699 ± 0.116
0.611GluTrp: 0.611 ± 0.042
2.875GluTyr: 2.875 ± 0.084
0.0GluXaa: 0.0 ± 0.0
Phe
2.283PheAla: 2.283 ± 0.069
0.469PheCys: 0.469 ± 0.034
1.8PheAsp: 1.8 ± 0.065
2.587PheGlu: 2.587 ± 0.085
1.567PhePhe: 1.567 ± 0.072
2.521PheGly: 2.521 ± 0.091
0.814PheHis: 0.814 ± 0.04
4.054PheIle: 4.054 ± 0.115
3.596PheLys: 3.596 ± 0.096
3.729PheLeu: 3.729 ± 0.108
1.036PheMet: 1.036 ± 0.057
1.864PheAsn: 1.864 ± 0.063
1.439PhePro: 1.439 ± 0.058
1.004PheGln: 1.004 ± 0.054
1.556PheArg: 1.556 ± 0.059
2.196PheSer: 2.196 ± 0.073
1.988PheThr: 1.988 ± 0.074
1.933PheVal: 1.933 ± 0.068
0.293PheTrp: 0.293 ± 0.031
1.416PheTyr: 1.416 ± 0.049
0.0PheXaa: 0.0 ± 0.0
Gly
5.007GlyAla: 5.007 ± 0.147
0.986GlyCys: 0.986 ± 0.054
3.697GlyAsp: 3.697 ± 0.109
5.2GlyGlu: 5.2 ± 0.135
3.262GlyPhe: 3.262 ± 0.099
4.978GlyGly: 4.978 ± 0.136
1.318GlyHis: 1.318 ± 0.064
6.863GlyIle: 6.863 ± 0.114
5.506GlyLys: 5.506 ± 0.13
6.346GlyLeu: 6.346 ± 0.135
2.013GlyMet: 2.013 ± 0.065
2.308GlyAsn: 2.308 ± 0.083
2.338GlyPro: 2.338 ± 0.072
1.35GlyGln: 1.35 ± 0.053
3.447GlyArg: 3.447 ± 0.092
4.232GlySer: 4.232 ± 0.094
3.774GlyThr: 3.774 ± 0.109
5.062GlyVal: 5.062 ± 0.131
0.595GlyTrp: 0.595 ± 0.038
2.731GlyTyr: 2.731 ± 0.082
0.0GlyXaa: 0.0 ± 0.0
His
1.185HisAla: 1.185 ± 0.061
0.281HisCys: 0.281 ± 0.023
0.906HisAsp: 0.906 ± 0.045
1.393HisGlu: 1.393 ± 0.05
0.691HisPhe: 0.691 ± 0.041
1.485HisGly: 1.485 ± 0.065
0.437HisHis: 0.437 ± 0.031
1.604HisIle: 1.604 ± 0.056
1.059HisLys: 1.059 ± 0.053
1.906HisLeu: 1.906 ± 0.067
0.4HisMet: 0.4 ± 0.027
0.579HisAsn: 0.579 ± 0.039
1.238HisPro: 1.238 ± 0.051
0.499HisGln: 0.499 ± 0.031
0.922HisArg: 0.922 ± 0.046
0.824HisSer: 0.824 ± 0.044
0.883HisThr: 0.883 ± 0.045
1.164HisVal: 1.164 ± 0.054
0.135HisTrp: 0.135 ± 0.017
0.684HisTyr: 0.684 ± 0.043
0.0HisXaa: 0.0 ± 0.0
Ile
6.261IleAla: 6.261 ± 0.143
1.071IleCys: 1.071 ± 0.051
4.893IleAsp: 4.893 ± 0.103
7.926IleGlu: 7.926 ± 0.143
3.72IlePhe: 3.72 ± 0.116
7.178IleGly: 7.178 ± 0.148
1.695IleHis: 1.695 ± 0.064
11.726IleIle: 11.726 ± 0.211
9.42IleLys: 9.42 ± 0.198
10.584IleLeu: 10.584 ± 0.198
2.352IleMet: 2.352 ± 0.064
3.626IleAsn: 3.626 ± 0.092
4.582IlePro: 4.582 ± 0.106
2.226IleGln: 2.226 ± 0.084
4.516IleArg: 4.516 ± 0.094
5.316IleSer: 5.316 ± 0.123
4.911IleThr: 4.911 ± 0.119
6.103IleVal: 6.103 ± 0.12
0.604IleTrp: 0.604 ± 0.036
2.789IleTyr: 2.789 ± 0.071
0.0IleXaa: 0.0 ± 0.0
Lys
5.234LysAla: 5.234 ± 0.115
1.009LysCys: 1.009 ± 0.069
4.719LysAsp: 4.719 ± 0.119
7.846LysGlu: 7.846 ± 0.157
3.063LysPhe: 3.063 ± 0.085
5.721LysGly: 5.721 ± 0.12
1.267LysHis: 1.267 ± 0.056
9.393LysIle: 9.393 ± 0.199
8.125LysLys: 8.125 ± 0.206
7.208LysLeu: 7.208 ± 0.155
2.21LysMet: 2.21 ± 0.079
3.786LysAsn: 3.786 ± 0.111
3.31LysPro: 3.31 ± 0.093
1.068LysGln: 1.068 ± 0.05
4.651LysArg: 4.651 ± 0.123
3.887LysSer: 3.887 ± 0.106
4.152LysThr: 4.152 ± 0.118
5.582LysVal: 5.582 ± 0.109
0.641LysTrp: 0.641 ± 0.04
2.704LysTyr: 2.704 ± 0.094
0.0LysXaa: 0.0 ± 0.0
Leu
6.446LeuAla: 6.446 ± 0.142
0.924LeuCys: 0.924 ± 0.052
5.085LeuAsp: 5.085 ± 0.112
8.418LeuGlu: 8.418 ± 0.155
3.267LeuPhe: 3.267 ± 0.124
6.652LeuGly: 6.652 ± 0.152
1.471LeuHis: 1.471 ± 0.059
8.452LeuIle: 8.452 ± 0.161
9.642LeuLys: 9.642 ± 0.186
8.638LeuLeu: 8.638 ± 0.188
2.276LeuMet: 2.276 ± 0.075
3.637LeuAsn: 3.637 ± 0.097
3.765LeuPro: 3.765 ± 0.103
2.134LeuGln: 2.134 ± 0.081
4.227LeuArg: 4.227 ± 0.1
5.376LeuSer: 5.376 ± 0.107
4.589LeuThr: 4.589 ± 0.118
5.273LeuVal: 5.273 ± 0.137
0.647LeuTrp: 0.647 ± 0.039
2.736LeuTyr: 2.736 ± 0.092
0.0LeuXaa: 0.0 ± 0.0
Met
1.954MetAla: 1.954 ± 0.069
0.229MetCys: 0.229 ± 0.022
1.608MetAsp: 1.608 ± 0.065
2.416MetGlu: 2.416 ± 0.081
0.727MetPhe: 0.727 ± 0.042
2.093MetGly: 2.093 ± 0.072
0.339MetHis: 0.339 ± 0.029
2.606MetIle: 2.606 ± 0.085
2.464MetLys: 2.464 ± 0.081
1.949MetLeu: 1.949 ± 0.074
0.602MetMet: 0.602 ± 0.039
0.952MetAsn: 0.952 ± 0.044
0.883MetPro: 0.883 ± 0.044
0.547MetGln: 0.547 ± 0.033
1.279MetArg: 1.279 ± 0.057
1.123MetSer: 1.123 ± 0.047
1.153MetThr: 1.153 ± 0.052
1.752MetVal: 1.752 ± 0.065
0.14MetTrp: 0.14 ± 0.019
0.49MetTyr: 0.49 ± 0.033
0.0MetXaa: 0.0 ± 0.0
Asn
2.269AsnAla: 2.269 ± 0.083
0.444AsnCys: 0.444 ± 0.03
1.494AsnAsp: 1.494 ± 0.069
2.242AsnGlu: 2.242 ± 0.071
1.569AsnPhe: 1.569 ± 0.063
2.564AsnGly: 2.564 ± 0.09
0.59AsnHis: 0.59 ± 0.04
5.081AsnIle: 5.081 ± 0.114
2.535AsnLys: 2.535 ± 0.083
4.253AsnLeu: 4.253 ± 0.106
1.082AsnMet: 1.082 ± 0.051
1.491AsnAsn: 1.491 ± 0.087
2.665AsnPro: 2.665 ± 0.073
0.84AsnGln: 0.84 ± 0.045
1.837AsnArg: 1.837 ± 0.071
1.835AsnSer: 1.835 ± 0.067
1.926AsnThr: 1.926 ± 0.08
2.722AsnVal: 2.722 ± 0.081
0.355AsnTrp: 0.355 ± 0.03
1.389AsnTyr: 1.389 ± 0.07
0.0AsnXaa: 0.0 ± 0.0
Pro
2.185ProAla: 2.185 ± 0.058
0.437ProCys: 0.437 ± 0.03
2.102ProAsp: 2.102 ± 0.072
4.008ProGlu: 4.008 ± 0.121
1.761ProPhe: 1.761 ± 0.072
3.189ProGly: 3.189 ± 0.1
1.018ProHis: 1.018 ± 0.053
3.804ProIle: 3.804 ± 0.105
2.649ProLys: 2.649 ± 0.085
4.072ProLeu: 4.072 ± 0.096
0.869ProMet: 0.869 ± 0.049
1.519ProAsn: 1.519 ± 0.057
1.963ProPro: 1.963 ± 0.064
1.132ProGln: 1.132 ± 0.047
2.116ProArg: 2.116 ± 0.066
2.724ProSer: 2.724 ± 0.084
2.416ProThr: 2.416 ± 0.073
2.747ProVal: 2.747 ± 0.09
0.398ProTrp: 0.398 ± 0.027
1.679ProTyr: 1.679 ± 0.059
0.0ProXaa: 0.0 ± 0.0
Gln
1.311GlnAla: 1.311 ± 0.06
0.231GlnCys: 0.231 ± 0.025
0.986GlnAsp: 0.986 ± 0.046
1.91GlnGlu: 1.91 ± 0.066
0.698GlnPhe: 0.698 ± 0.039
1.409GlnGly: 1.409 ± 0.055
0.38GlnHis: 0.38 ± 0.03
1.919GlnIle: 1.919 ± 0.072
1.867GlnLys: 1.867 ± 0.07
1.901GlnLeu: 1.901 ± 0.063
0.606GlnMet: 0.606 ± 0.035
0.769GlnAsn: 0.769 ± 0.043
0.789GlnPro: 0.789 ± 0.042
0.478GlnGln: 0.478 ± 0.034
1.331GlnArg: 1.331 ± 0.055
0.874GlnSer: 0.874 ± 0.044
0.961GlnThr: 0.961 ± 0.053
1.421GlnVal: 1.421 ± 0.05
0.192GlnTrp: 0.192 ± 0.025
0.641GlnTyr: 0.641 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
3.047ArgAla: 3.047 ± 0.087
0.519ArgCys: 0.519 ± 0.042
2.272ArgAsp: 2.272 ± 0.078
4.406ArgGlu: 4.406 ± 0.108
1.777ArgPhe: 1.777 ± 0.071
3.333ArgGly: 3.333 ± 0.113
0.743ArgHis: 0.743 ± 0.042
5.259ArgIle: 5.259 ± 0.095
4.076ArgLys: 4.076 ± 0.112
4.742ArgLeu: 4.742 ± 0.103
1.395ArgMet: 1.395 ± 0.05
1.439ArgAsn: 1.439 ± 0.06
1.661ArgPro: 1.661 ± 0.073
0.938ArgGln: 0.938 ± 0.049
3.001ArgArg: 3.001 ± 0.091
2.324ArgSer: 2.324 ± 0.073
1.922ArgThr: 1.922 ± 0.07
3.15ArgVal: 3.15 ± 0.098
0.387ArgTrp: 0.387 ± 0.029
1.501ArgTyr: 1.501 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
3.127SerAla: 3.127 ± 0.093
0.567SerCys: 0.567 ± 0.044
2.313SerAsp: 2.313 ± 0.061
3.395SerGlu: 3.395 ± 0.084
2.258SerPhe: 2.258 ± 0.073
3.676SerGly: 3.676 ± 0.095
1.158SerHis: 1.158 ± 0.049
5.197SerIle: 5.197 ± 0.114
4.131SerLys: 4.131 ± 0.096
5.492SerLeu: 5.492 ± 0.127
1.453SerMet: 1.453 ± 0.052
2.013SerAsn: 2.013 ± 0.067
2.427SerPro: 2.427 ± 0.07
1.446SerGln: 1.446 ± 0.052
2.569SerArg: 2.569 ± 0.094
3.084SerSer: 3.084 ± 0.099
2.871SerThr: 2.871 ± 0.087
2.759SerVal: 2.759 ± 0.088
0.43SerTrp: 0.43 ± 0.034
1.999SerTyr: 1.999 ± 0.068
0.0SerXaa: 0.0 ± 0.0
Thr
3.267ThrAla: 3.267 ± 0.107
0.654ThrCys: 0.654 ± 0.041
2.301ThrAsp: 2.301 ± 0.076
2.766ThrGlu: 2.766 ± 0.088
2.008ThrPhe: 2.008 ± 0.074
4.571ThrGly: 4.571 ± 0.118
0.936ThrHis: 0.936 ± 0.05
5.195ThrIle: 5.195 ± 0.12
2.779ThrLys: 2.779 ± 0.083
4.875ThrLeu: 4.875 ± 0.12
1.162ThrMet: 1.162 ± 0.048
1.686ThrAsn: 1.686 ± 0.074
2.88ThrPro: 2.88 ± 0.085
1.057ThrGln: 1.057 ± 0.046
2.713ThrArg: 2.713 ± 0.081
3.203ThrSer: 3.203 ± 0.083
2.793ThrThr: 2.793 ± 0.089
3.106ThrVal: 3.106 ± 0.093
0.448ThrTrp: 0.448 ± 0.044
1.626ThrTyr: 1.626 ± 0.069
0.0ThrXaa: 0.0 ± 0.0
Val
4.493ValAla: 4.493 ± 0.115
0.705ValCys: 0.705 ± 0.042
3.962ValAsp: 3.962 ± 0.093
5.053ValGlu: 5.053 ± 0.138
2.258ValPhe: 2.258 ± 0.076
4.461ValGly: 4.461 ± 0.108
1.13ValHis: 1.13 ± 0.053
5.566ValIle: 5.566 ± 0.126
5.662ValLys: 5.662 ± 0.119
5.035ValLeu: 5.035 ± 0.126
1.425ValMet: 1.425 ± 0.058
2.457ValAsn: 2.457 ± 0.085
2.562ValPro: 2.562 ± 0.078
1.217ValGln: 1.217 ± 0.052
2.313ValArg: 2.313 ± 0.083
3.367ValSer: 3.367 ± 0.094
3.445ValThr: 3.445 ± 0.1
3.852ValVal: 3.852 ± 0.111
0.407ValTrp: 0.407 ± 0.032
2.223ValTyr: 2.223 ± 0.079
0.0ValXaa: 0.0 ± 0.0
Trp
0.49TrpAla: 0.49 ± 0.04
0.103TrpCys: 0.103 ± 0.016
0.531TrpAsp: 0.531 ± 0.038
0.597TrpGlu: 0.597 ± 0.039
0.293TrpPhe: 0.293 ± 0.029
0.547TrpGly: 0.547 ± 0.043
0.112TrpHis: 0.112 ± 0.016
0.817TrpIle: 0.817 ± 0.055
0.567TrpLys: 0.567 ± 0.035
0.673TrpLeu: 0.673 ± 0.042
0.242TrpMet: 0.242 ± 0.026
0.371TrpAsn: 0.371 ± 0.033
0.213TrpPro: 0.213 ± 0.023
0.208TrpGln: 0.208 ± 0.02
0.389TrpArg: 0.389 ± 0.026
0.35TrpSer: 0.35 ± 0.031
0.368TrpThr: 0.368 ± 0.035
0.419TrpVal: 0.419 ± 0.028
0.11TrpTrp: 0.11 ± 0.014
0.281TrpTyr: 0.281 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.97TyrAla: 1.97 ± 0.071
0.464TyrCys: 0.464 ± 0.034
1.839TyrAsp: 1.839 ± 0.062
2.24TyrGlu: 2.24 ± 0.078
1.352TyrPhe: 1.352 ± 0.056
2.564TyrGly: 2.564 ± 0.08
0.901TyrHis: 0.901 ± 0.046
2.997TyrIle: 2.997 ± 0.095
2.894TyrLys: 2.894 ± 0.083
3.189TyrLeu: 3.189 ± 0.088
0.869TyrMet: 0.869 ± 0.045
1.812TyrAsn: 1.812 ± 0.071
1.599TyrPro: 1.599 ± 0.064
0.837TyrGln: 0.837 ± 0.047
1.519TyrArg: 1.519 ± 0.064
1.809TyrSer: 1.809 ± 0.064
1.862TyrThr: 1.862 ± 0.069
1.69TyrVal: 1.69 ± 0.064
0.334TyrTrp: 0.334 ± 0.028
1.572TyrTyr: 1.572 ± 0.065
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1556 proteins (437150 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski