Mycobacterium phage Ruby

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 99 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385DZZ5|A0A385DZZ5_9CAUD Lysin A OS=Mycobacterium phage Ruby OX=2301713 GN=31 PE=4 SV=1
MM1 pKa = 7.59SGDD4 pKa = 3.12INAEE8 pKa = 3.61GFIRR12 pKa = 11.84YY13 pKa = 9.51GGDD16 pKa = 3.52CTCGAIYY23 pKa = 9.12TYY25 pKa = 10.64GGHH28 pKa = 7.08AEE30 pKa = 4.57PGSFDD35 pKa = 4.3PFCPDD40 pKa = 2.7HH41 pKa = 7.27GEE43 pKa = 4.02AAVVATGEE51 pKa = 4.19EE52 pKa = 4.15AA53 pKa = 3.78

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385E2L7|A0A385E2L7_9CAUD Uncharacterized protein OS=Mycobacterium phage Ruby OX=2301713 GN=73 PE=4 SV=1
MM1 pKa = 7.47ARR3 pKa = 11.84QRR5 pKa = 11.84IPAPNPITGLSRR17 pKa = 11.84EE18 pKa = 4.16GLAVDD23 pKa = 4.22ATQEE27 pKa = 3.52QWRR30 pKa = 11.84PVVGYY35 pKa = 9.66EE36 pKa = 4.2GMYY39 pKa = 10.11EE40 pKa = 4.0VSDD43 pKa = 3.91LGRR46 pKa = 11.84VRR48 pKa = 11.84SVDD51 pKa = 2.96RR52 pKa = 11.84CVVTKK57 pKa = 10.55SGPRR61 pKa = 11.84TYY63 pKa = 10.39RR64 pKa = 11.84GRR66 pKa = 11.84LLHH69 pKa = 5.53QHH71 pKa = 6.62PDD73 pKa = 2.75GRR75 pKa = 11.84GYY77 pKa = 11.11LRR79 pKa = 11.84ASLSRR84 pKa = 11.84VGDD87 pKa = 3.61KK88 pKa = 10.65PRR90 pKa = 11.84MFKK93 pKa = 9.06VHH95 pKa = 6.88RR96 pKa = 11.84LVLEE100 pKa = 4.17AFVGPRR106 pKa = 11.84PGNLSGCHH114 pKa = 5.25NNGINTDD121 pKa = 3.3NRR123 pKa = 11.84LEE125 pKa = 3.91NLRR128 pKa = 11.84WDD130 pKa = 3.55SHH132 pKa = 4.99TEE134 pKa = 3.56NMLDD138 pKa = 3.6VVRR141 pKa = 11.84HH142 pKa = 5.38GRR144 pKa = 11.84HH145 pKa = 5.83HH146 pKa = 5.07YY147 pKa = 10.47AKK149 pKa = 10.07RR150 pKa = 11.84DD151 pKa = 3.55RR152 pKa = 11.84CPKK155 pKa = 9.06GHH157 pKa = 6.52VFNEE161 pKa = 4.08PNTLISPRR169 pKa = 11.84GARR172 pKa = 11.84VCRR175 pKa = 11.84TCQKK179 pKa = 10.44QYY181 pKa = 10.88NDD183 pKa = 3.98RR184 pKa = 11.84YY185 pKa = 9.46YY186 pKa = 10.02QTACDD191 pKa = 3.81EE192 pKa = 3.94KK193 pKa = 10.6RR194 pKa = 11.84ARR196 pKa = 11.84GWIPKK201 pKa = 9.63RR202 pKa = 11.84EE203 pKa = 3.94RR204 pKa = 11.84TKK206 pKa = 10.93CPLGHH211 pKa = 6.7EE212 pKa = 3.92YY213 pKa = 10.61DD214 pKa = 3.52YY215 pKa = 11.34FYY217 pKa = 11.65VNKK220 pKa = 10.32RR221 pKa = 11.84NGKK224 pKa = 6.05VTRR227 pKa = 11.84HH228 pKa = 6.06CKK230 pKa = 7.53TCRR233 pKa = 11.84AQNHH237 pKa = 5.53RR238 pKa = 11.84NFKK241 pKa = 9.89KK242 pKa = 10.08RR243 pKa = 11.84RR244 pKa = 11.84AGASCVV250 pKa = 3.28

Molecular weight: 28.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

99

0

99

18570

39

1168

187.6

20.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.226 ± 0.443

1.309 ± 0.161

6.554 ± 0.24

5.999 ± 0.318

3.123 ± 0.222

8.826 ± 0.548

2.31 ± 0.195

4.233 ± 0.206

3.489 ± 0.203

7.351 ± 0.227

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.197 ± 0.138

3.533 ± 0.179

6.123 ± 0.208

3.473 ± 0.164

7.076 ± 0.425

5.67 ± 0.265

6.597 ± 0.271

7.135 ± 0.26

2.283 ± 0.126

2.493 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski