Buchnera aphidicola (Thelaxes californica)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Erwiniaceae; Buchnera; Buchnera aphidicola

Average proteome isoelectric point is 8.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6YLA5|A0A4D6YLA5_9GAMM Dual-specificity RNA methyltransferase RlmN OS=Buchnera aphidicola (Thelaxes californica) OX=1315998 GN=rlmN PE=3 SV=1
MM1 pKa = 7.23QFEE4 pKa = 4.34EE5 pKa = 4.75KK6 pKa = 10.47KK7 pKa = 10.47IIEE10 pKa = 4.24NLFEE14 pKa = 3.98RR15 pKa = 11.84LKK17 pKa = 10.98NVEE20 pKa = 4.26EE21 pKa = 4.21KK22 pKa = 10.97CPEE25 pKa = 4.12KK26 pKa = 10.45NTLANNFINEE36 pKa = 4.83CIQKK40 pKa = 9.63NPNSIYY46 pKa = 10.72YY47 pKa = 9.93IIQTVLIQEE56 pKa = 4.26DD57 pKa = 4.45VIKK60 pKa = 10.59KK61 pKa = 9.55LNEE64 pKa = 4.0RR65 pKa = 11.84IIALEE70 pKa = 3.7NDD72 pKa = 2.5ISLFKK77 pKa = 10.84KK78 pKa = 10.35NNNIQEE84 pKa = 4.06PSFLKK89 pKa = 9.61NTEE92 pKa = 3.85NAIKK96 pKa = 9.32STQKK100 pKa = 11.06LDD102 pKa = 3.35TKK104 pKa = 10.81KK105 pKa = 10.52NQNTISKK112 pKa = 9.64NYY114 pKa = 10.14DD115 pKa = 3.09SAFYY119 pKa = 9.46EE120 pKa = 4.63QKK122 pKa = 10.89NPMNKK127 pKa = 9.45GCNSSISSGGVSSFLGSAAQTAAGVAGGIFMGNILNNLFHH167 pKa = 7.07SDD169 pKa = 3.9KK170 pKa = 10.77EE171 pKa = 3.87ISTPIDD177 pKa = 3.13INNQNDD183 pKa = 3.32LSKK186 pKa = 10.78ISNTDD191 pKa = 3.31SIKK194 pKa = 10.72KK195 pKa = 10.28VNDD198 pKa = 3.6DD199 pKa = 4.0FLSEE203 pKa = 3.95EE204 pKa = 4.92DD205 pKa = 4.23KK206 pKa = 11.21IQNNEE211 pKa = 4.0NKK213 pKa = 10.19NLDD216 pKa = 3.32HH217 pKa = 7.73DD218 pKa = 4.42YY219 pKa = 11.43EE220 pKa = 4.59NNHH223 pKa = 5.93EE224 pKa = 4.25EE225 pKa = 4.33ANNEE229 pKa = 3.56YY230 pKa = 10.82DD231 pKa = 3.82EE232 pKa = 5.62YY233 pKa = 11.55DD234 pKa = 5.64DD235 pKa = 5.67EE236 pKa = 7.4DD237 pKa = 4.95EE238 pKa = 6.37DD239 pKa = 6.46DD240 pKa = 4.64DD241 pKa = 6.49DD242 pKa = 5.52YY243 pKa = 12.18YY244 pKa = 11.42EE245 pKa = 4.23NHH247 pKa = 7.75DD248 pKa = 5.48DD249 pKa = 5.14NMLDD253 pKa = 3.82IDD255 pKa = 4.06NQGDD259 pKa = 3.62EE260 pKa = 4.48LLL262 pKa = 4.73

Molecular weight: 30.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6YJD9|A0A4D6YJD9_9GAMM 50S ribosomal protein L1 OS=Buchnera aphidicola (Thelaxes californica) OX=1315998 GN=rplA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.58RR3 pKa = 11.84TFQPSLLKK11 pKa = 10.53RR12 pKa = 11.84NRR14 pKa = 11.84LHH16 pKa = 6.97GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.24NGRR28 pKa = 11.84HH29 pKa = 5.07ILSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84NKK37 pKa = 10.06CRR39 pKa = 11.84IKK41 pKa = 10.47LTVSSKK47 pKa = 11.07

Molecular weight: 5.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

455

0

455

147751

38

1415

324.7

37.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.907 ± 0.102

1.346 ± 0.039

3.894 ± 0.078

4.91 ± 0.102

5.247 ± 0.111

5.015 ± 0.112

2.355 ± 0.047

12.648 ± 0.126

10.704 ± 0.161

9.519 ± 0.088

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.246 ± 0.048

7.858 ± 0.133

2.898 ± 0.052

3.794 ± 0.065

3.034 ± 0.092

6.464 ± 0.076

4.775 ± 0.06

4.625 ± 0.095

0.84 ± 0.044

3.923 ± 0.074
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski