Rhodobacteraceae bacterium KMS-5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3633 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M0QU51|A0A6M0QU51_9RHOB HutD family protein OS=Rhodobacteraceae bacterium KMS-5 OX=2710650 GN=G4Z14_07655 PE=4 SV=1
MM1 pKa = 7.86ADD3 pKa = 4.29DD4 pKa = 5.16IYY6 pKa = 11.04TIDD9 pKa = 3.65ALATGSEE16 pKa = 4.74TITITDD22 pKa = 4.65DD23 pKa = 3.53GTGSDD28 pKa = 3.08WLVYY32 pKa = 9.51SGSYY36 pKa = 10.51ASYY39 pKa = 11.62AEE41 pKa = 4.87IDD43 pKa = 3.91LSWFVDD49 pKa = 3.48SGISQSAEE57 pKa = 3.25GLYY60 pKa = 8.83FTVTGGHH67 pKa = 6.02RR68 pKa = 11.84LVINGLIEE76 pKa = 4.01NARR79 pKa = 11.84GTTAEE84 pKa = 4.22DD85 pKa = 3.84WISGNEE91 pKa = 3.96ADD93 pKa = 4.11NLLYY97 pKa = 10.98GDD99 pKa = 5.04FDD101 pKa = 3.8ATGAGGNDD109 pKa = 4.49TINGDD114 pKa = 3.49AGRR117 pKa = 11.84DD118 pKa = 3.67TIYY121 pKa = 10.89GGSGLDD127 pKa = 3.66SLEE130 pKa = 3.95GSYY133 pKa = 11.51DD134 pKa = 3.85DD135 pKa = 6.2DD136 pKa = 4.92LLHH139 pKa = 7.29GDD141 pKa = 4.12GGADD145 pKa = 3.37TVSGGAGNDD154 pKa = 3.39TVLGGEE160 pKa = 4.38GADD163 pKa = 3.39WLYY166 pKa = 11.57GGADD170 pKa = 3.28ARR172 pKa = 11.84DD173 pKa = 3.71VVSYY177 pKa = 11.34ADD179 pKa = 3.64STDD182 pKa = 2.97GVYY185 pKa = 10.04IEE187 pKa = 4.36ITYY190 pKa = 10.76GSTTIATSGFAEE202 pKa = 4.4GDD204 pKa = 3.67HH205 pKa = 7.03LNGFTDD211 pKa = 5.62AIGSAYY217 pKa = 10.07GDD219 pKa = 4.34RR220 pKa = 11.84ITDD223 pKa = 3.67TVVNTIAFGYY233 pKa = 8.42NANRR237 pKa = 11.84FYY239 pKa = 11.38GGGGADD245 pKa = 4.42LLNMGGGNDD254 pKa = 3.39SGYY257 pKa = 11.18GGDD260 pKa = 4.92GRR262 pKa = 11.84DD263 pKa = 3.5SVNGGVGDD271 pKa = 4.29DD272 pKa = 4.18LLFGGGGNDD281 pKa = 4.47DD282 pKa = 3.56VDD284 pKa = 4.21GGTGADD290 pKa = 3.14VVYY293 pKa = 10.81GGDD296 pKa = 3.52GYY298 pKa = 11.79NRR300 pKa = 11.84LIGGAGQDD308 pKa = 3.28RR309 pKa = 11.84VIGGGLRR316 pKa = 11.84EE317 pKa = 4.08TLYY320 pKa = 11.24GGAGDD325 pKa = 4.19DD326 pKa = 3.69TLLAGGGNDD335 pKa = 4.33LLFANEE341 pKa = 3.94GNDD344 pKa = 3.27RR345 pKa = 11.84VLGGAGDD352 pKa = 4.15DD353 pKa = 4.41LLFGLAGKK361 pKa = 8.18DD362 pKa = 3.46TLIGGAGADD371 pKa = 3.31EE372 pKa = 4.49FRR374 pKa = 11.84FISATDD380 pKa = 3.59GGSDD384 pKa = 3.07AASRR388 pKa = 11.84DD389 pKa = 3.29VVMDD393 pKa = 4.17FNRR396 pKa = 11.84SQGDD400 pKa = 3.54HH401 pKa = 6.37LVFSIAAASLDD412 pKa = 4.05FIGQQSFTGQAGEE425 pKa = 3.89VRR427 pKa = 11.84FIVTAQGTRR436 pKa = 11.84AFADD440 pKa = 3.46LDD442 pKa = 3.59GDD444 pKa = 4.82RR445 pKa = 11.84ITDD448 pKa = 3.29QAFDD452 pKa = 3.63VLGVSLLQEE461 pKa = 4.04SDD463 pKa = 3.75FVLL466 pKa = 4.49

Molecular weight: 47.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M0QYF1|A0A6M0QYF1_9RHOB Lytic transglycosylase domain-containing protein OS=Rhodobacteraceae bacterium KMS-5 OX=2710650 GN=G4Z14_17155 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.21GRR39 pKa = 11.84HH40 pKa = 5.51KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3633

0

3633

1152034

30

2141

317.1

34.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.654 ± 0.067

0.872 ± 0.013

5.663 ± 0.035

5.59 ± 0.037

3.623 ± 0.027

9.174 ± 0.051

2.094 ± 0.021

4.765 ± 0.029

3.069 ± 0.034

10.432 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.706 ± 0.02

2.375 ± 0.025

5.25 ± 0.034

3.051 ± 0.021

6.88 ± 0.041

4.704 ± 0.028

5.289 ± 0.031

7.294 ± 0.034

1.441 ± 0.02

2.076 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski