Heliothis zea nudivirus

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Nudiviridae; Betanudivirus

Average proteome isoelectric point is 7.17

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q8JKG6|Q8JKG6_9VIRU Orf147 OS=Heliothis zea nudivirus OX=29250 GN=orf147 PE=4 SV=1
MM1 pKa = 7.44SLLRR5 pKa = 11.84KK6 pKa = 7.25PTSEE10 pKa = 4.23TEE12 pKa = 3.81FDD14 pKa = 4.94LEE16 pKa = 4.72DD17 pKa = 3.61LTISLSDD24 pKa = 3.68YY25 pKa = 10.3PLSNHH30 pKa = 6.83SIVDD34 pKa = 4.69DD35 pKa = 3.95IQHH38 pKa = 6.69LMGDD42 pKa = 4.81LYY44 pKa = 10.54YY45 pKa = 10.62QCNDD49 pKa = 3.09STCRR53 pKa = 11.84CKK55 pKa = 10.41IEE57 pKa = 4.5IIVGFLMGFILGSVLARR74 pKa = 11.84LTSGYY79 pKa = 10.15AANAHH84 pKa = 6.88HH85 pKa = 6.75SRR87 pKa = 11.84QDD89 pKa = 3.27QTDD92 pKa = 3.64DD93 pKa = 3.66EE94 pKa = 4.85GLGTSTEE101 pKa = 4.11EE102 pKa = 5.15GEE104 pKa = 4.88NADD107 pKa = 5.8DD108 pKa = 6.37DD109 pKa = 5.38DD110 pKa = 6.87DD111 pKa = 6.07DD112 pKa = 5.1YY113 pKa = 12.16SSSSEE118 pKa = 3.83EE119 pKa = 3.8ATDD122 pKa = 3.74YY123 pKa = 11.23GASTDD128 pKa = 4.19EE129 pKa = 5.32DD130 pKa = 5.07DD131 pKa = 5.45DD132 pKa = 5.73DD133 pKa = 4.97EE134 pKa = 5.47SASEE138 pKa = 4.0WGNEE142 pKa = 3.84VHH144 pKa = 7.39LDD146 pKa = 3.4

Molecular weight:
16.12 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q8JKS1|Q8JKS1_9VIRU Arginine-rich protein a209R OS=Heliothis zea nudivirus OX=29250 GN=orf40 PE=4 SV=1
MM1 pKa = 7.5RR2 pKa = 11.84TPLNVKK8 pKa = 9.63PRR10 pKa = 11.84LKK12 pKa = 10.12QQSAKK17 pKa = 10.1RR18 pKa = 11.84RR19 pKa = 11.84LKK21 pKa = 10.52LLPNVKK27 pKa = 9.54PRR29 pKa = 11.84LKK31 pKa = 10.18RR32 pKa = 11.84QPNSKK37 pKa = 9.86RR38 pKa = 11.84RR39 pKa = 11.84LKK41 pKa = 10.71LPPNVKK47 pKa = 9.65PRR49 pKa = 11.84LKK51 pKa = 10.18RR52 pKa = 11.84QPNSKK57 pKa = 9.94RR58 pKa = 11.84RR59 pKa = 11.84LKK61 pKa = 11.0LPLNAKK67 pKa = 9.87RR68 pKa = 11.84RR69 pKa = 11.84LKK71 pKa = 10.16QPKK74 pKa = 9.37PNVKK78 pKa = 9.9LRR80 pKa = 11.84LKK82 pKa = 10.28QPPNVKK88 pKa = 9.78RR89 pKa = 11.84RR90 pKa = 11.84LKK92 pKa = 10.64LPPNAKK98 pKa = 9.64RR99 pKa = 11.84RR100 pKa = 11.84LKK102 pKa = 10.95LPLNAKK108 pKa = 9.85RR109 pKa = 11.84RR110 pKa = 11.84LKK112 pKa = 10.91LPLNVKK118 pKa = 9.75PRR120 pKa = 11.84LKK122 pKa = 10.1QQNEE126 pKa = 3.91RR127 pKa = 11.84PMLKK131 pKa = 9.89QPPNVKK137 pKa = 10.02LRR139 pKa = 11.84LRR141 pKa = 11.84QQNEE145 pKa = 3.79RR146 pKa = 11.84PMLKK150 pKa = 9.89QPPNVKK156 pKa = 10.02LRR158 pKa = 11.84LRR160 pKa = 11.84QPNAKK165 pKa = 9.62RR166 pKa = 11.84RR167 pKa = 11.84LKK169 pKa = 10.64LLNEE173 pKa = 5.04KK174 pKa = 9.26EE175 pKa = 4.14LQMNN179 pKa = 4.47

Molecular weight:
21.58 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

154

0

154

52489

44

1585

340.8

38.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.152 ± 0.152

2.103 ± 0.121

6.338 ± 0.189

5.715 ± 0.207

3.742 ± 0.13

4.241 ± 0.126

2.351 ± 0.106

5.546 ± 0.13

6.716 ± 0.216

8.329 ± 0.307

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.705 ± 0.101

6.958 ± 0.283

4.494 ± 0.227

3.241 ± 0.121

4.851 ± 0.162

9.048 ± 0.378

7.278 ± 0.347

6.542 ± 0.159

0.583 ± 0.051

4.048 ± 0.173

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski