Microbacterium phage Akoni

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Eekayvirinae; Akonivirus; Microbacterium virus Akoni

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6TBY4|A0A4D6TBY4_9CAUD Uncharacterized protein OS=Microbacterium phage Akoni OX=2565510 GN=48 PE=4 SV=1
MM1 pKa = 7.61GYY3 pKa = 10.42KK4 pKa = 9.61IVTEE8 pKa = 4.18VEE10 pKa = 4.16VAEE13 pKa = 4.49GTDD16 pKa = 3.34AVGISSVLSYY26 pKa = 11.31VLNTSLQGVPGVTSVSITEE45 pKa = 3.87LTAIEE50 pKa = 4.45EE51 pKa = 4.75GSTNAA56 pKa = 4.73

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6T8A3|A0A4D6T8A3_9CAUD Uncharacterized protein OS=Microbacterium phage Akoni OX=2565510 GN=53 PE=4 SV=1
MM1 pKa = 7.77WYY3 pKa = 10.69LMPLARR9 pKa = 11.84HH10 pKa = 4.76KK11 pKa = 9.1TVRR14 pKa = 11.84QALQAVEE21 pKa = 5.35HH22 pKa = 6.34SPGWPNDD29 pKa = 3.52SFEE32 pKa = 5.15SRR34 pKa = 11.84MNMPVHH40 pKa = 6.34EE41 pKa = 4.29MVARR45 pKa = 11.84NLFDD49 pKa = 3.27IANNGDD55 pKa = 3.84PSNQASMTRR64 pKa = 11.84ALRR67 pKa = 11.84AARR70 pKa = 11.84IILDD74 pKa = 3.57RR75 pKa = 11.84LTGTRR80 pKa = 11.84RR81 pKa = 11.84MGTHH85 pKa = 6.08PAVRR89 pKa = 11.84NQKK92 pKa = 7.32QVKK95 pKa = 9.12IIDD98 pKa = 3.68MTQMGGEE105 pKa = 4.02KK106 pKa = 10.24SEE108 pKa = 4.06

Molecular weight: 12.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

17069

39

4487

310.3

34.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.479 ± 0.406

0.434 ± 0.136

6.562 ± 0.215

6.544 ± 0.357

3.351 ± 0.135

7.634 ± 0.368

1.734 ± 0.166

4.845 ± 0.164

4.376 ± 0.396

8.231 ± 0.297

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.145

4.681 ± 0.181

4.728 ± 0.234

4.529 ± 0.299

5.941 ± 0.367

5.952 ± 0.235

6.989 ± 0.369

6.567 ± 0.243

1.881 ± 0.122

3.017 ± 0.222
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski