Legionella geestiana

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Legionellales; Legionellaceae; Legionella

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2282 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0W0TYH6|A0A0W0TYH6_9GAMM Uncharacterized protein OS=Legionella geestiana OX=45065 GN=E4T54_02610 PE=4 SV=1
MM1 pKa = 6.82NTNPEE6 pKa = 3.93ISDD9 pKa = 3.22ADD11 pKa = 3.4IRR13 pKa = 11.84FSTSAADD20 pKa = 3.94KK21 pKa = 9.78VASLITEE28 pKa = 4.04EE29 pKa = 4.6EE30 pKa = 4.12NPNLCLRR37 pKa = 11.84VYY39 pKa = 9.24ITGGGCSGFQYY50 pKa = 10.95GFTFDD55 pKa = 3.53EE56 pKa = 5.27TINDD60 pKa = 3.75DD61 pKa = 3.83DD62 pKa = 4.43FVIEE66 pKa = 4.57KK67 pKa = 10.02SCSNARR73 pKa = 11.84DD74 pKa = 3.77SVRR77 pKa = 11.84VLIDD81 pKa = 3.3SMSYY85 pKa = 10.49QYY87 pKa = 10.78LQSAEE92 pKa = 3.63IDD94 pKa = 3.8YY95 pKa = 11.56VEE97 pKa = 5.21GIGGAQFVIRR107 pKa = 11.84NPQAKK112 pKa = 6.07TTCGCGSSFSIEE124 pKa = 4.0EE125 pKa = 3.95

Molecular weight: 13.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0W0TNB5|A0A0W0TNB5_9GAMM Enoyl-CoA hydratase OS=Legionella geestiana OX=45065 GN=paaF PE=3 SV=1
MM1 pKa = 7.41GSCLRR6 pKa = 11.84GNDD9 pKa = 4.06RR10 pKa = 11.84IRR12 pKa = 11.84GVCRR16 pKa = 11.84GQNLSHH22 pKa = 6.29TVFPAQAGTHH32 pKa = 5.75SGCGTEE38 pKa = 4.33PAHH41 pKa = 6.39GTIQPAHH48 pKa = 5.43GTKK51 pKa = 9.49IHH53 pKa = 6.09VNPPARR59 pKa = 11.84KK60 pKa = 8.35KK61 pKa = 10.86CPVHH65 pKa = 6.72RR66 pKa = 11.84AQAHH70 pKa = 4.95PHH72 pKa = 5.01YY73 pKa = 10.97KK74 pKa = 8.69MLQMRR79 pKa = 11.84CRR81 pKa = 11.84VSLCALMHH89 pKa = 6.31NPSKK93 pKa = 10.79LHH95 pKa = 5.7VFASHH100 pKa = 6.39HH101 pKa = 6.24ALRR104 pKa = 11.84TLTKK108 pKa = 7.4YY109 pKa = 9.22TCMAHH114 pKa = 5.77VPCTITGTLPLKK126 pKa = 9.56TLLHH130 pKa = 6.48RR131 pKa = 11.84RR132 pKa = 11.84HH133 pKa = 6.44CLPLCLKK140 pKa = 10.12HH141 pKa = 7.19RR142 pKa = 11.84RR143 pKa = 11.84ICQGSSGYY151 pKa = 10.06LLKK154 pKa = 10.82RR155 pKa = 11.84SWW157 pKa = 3.07

Molecular weight: 17.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2282

0

2282

773064

50

3687

338.8

37.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.978 ± 0.063

1.326 ± 0.021

4.84 ± 0.035

6.068 ± 0.045

4.241 ± 0.029

6.662 ± 0.053

2.688 ± 0.031

5.486 ± 0.044

4.232 ± 0.045

11.304 ± 0.07

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.601 ± 0.025

3.682 ± 0.037

4.739 ± 0.04

3.835 ± 0.034

6.116 ± 0.041

6.223 ± 0.037

5.429 ± 0.032

6.579 ± 0.05

1.258 ± 0.019

2.709 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski