Circoviridae 16 LDMD-2013

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; environmental samples

Average proteome isoelectric point is 8.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5TNF0|S5TNF0_9CIRC Replication-associated protein OS=Circoviridae 16 LDMD-2013 OX=1379720 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84EE3 pKa = 3.75EE4 pKa = 3.89VRR6 pKa = 11.84IVGPLRR12 pKa = 11.84GPTLVSAVLITPAVNLHH29 pKa = 6.45DD30 pKa = 4.23VDD32 pKa = 3.29VHH34 pKa = 4.79TAHH37 pKa = 7.48RR38 pKa = 11.84GGVRR42 pKa = 11.84KK43 pKa = 9.58GHH45 pKa = 6.24DD46 pKa = 3.54TPDD49 pKa = 3.34LLRR52 pKa = 11.84IGDD55 pKa = 3.93VVVPIGVVACVVGGVAIRR73 pKa = 11.84TQVALKK79 pKa = 10.33RR80 pKa = 11.84HH81 pKa = 5.41VNLAGATNGLGARR94 pKa = 11.84VLVVQVEE101 pKa = 4.28VLGLKK106 pKa = 9.81DD107 pKa = 3.82VNVLTVEE114 pKa = 4.06VVPILRR120 pKa = 11.84IFEE123 pKa = 4.38SHH125 pKa = 5.79VSKK128 pKa = 10.0ATNRR132 pKa = 11.84FWKK135 pKa = 9.74AQNASTVYY143 pKa = 10.67EE144 pKa = 4.31SFLLANNVDD153 pKa = 5.15ANVEE157 pKa = 4.16WIRR160 pKa = 11.84TDD162 pKa = 4.17GEE164 pKa = 4.35VCMSLKK170 pKa = 10.18RR171 pKa = 11.84HH172 pKa = 5.5GRR174 pKa = 11.84RR175 pKa = 11.84QRR177 pKa = 11.84LVHH180 pKa = 6.4HH181 pKa = 6.69LAGGIVLNLKK191 pKa = 9.96VVVVLLVGGNVRR203 pKa = 11.84VVLLRR208 pKa = 11.84LCEE211 pKa = 3.85VRR213 pKa = 11.84NLVGTVVHH221 pKa = 6.77IGGPSGNTHH230 pKa = 7.0ASHH233 pKa = 6.87VLDD236 pKa = 3.86KK237 pKa = 11.54VVDD240 pKa = 4.1TEE242 pKa = 4.38THH244 pKa = 5.69GLLGIKK250 pKa = 9.85VRR252 pKa = 11.84TRR254 pKa = 11.84LKK256 pKa = 10.96AKK258 pKa = 9.98ILFAGLEE265 pKa = 4.01LAEE268 pKa = 4.53LCTLEE273 pKa = 4.92SLHH276 pKa = 7.02CSGEE280 pKa = 4.03LGDD283 pKa = 3.9ACGVLEE289 pKa = 4.19EE290 pKa = 4.98TGDD293 pKa = 3.18VWLALDD299 pKa = 4.79LRR301 pKa = 11.84DD302 pKa = 3.73GLKK305 pKa = 9.94TSTILCKK312 pKa = 10.03GTLSGSSLNCFNSTNTATSSXASSSLSS339 pKa = 3.35

Molecular weight: 36.0 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5SYF3|S5SYF3_9CIRC Replication-associated protein OS=Circoviridae 16 LDMD-2013 OX=1379720 PE=4 SV=1
MM1 pKa = 8.03PYY3 pKa = 8.88GTSRR7 pKa = 11.84KK8 pKa = 8.12RR9 pKa = 11.84TARR12 pKa = 11.84XATSRR17 pKa = 11.84RR18 pKa = 11.84VSAVKK23 pKa = 9.56TIQAAARR30 pKa = 11.84KK31 pKa = 9.03RR32 pKa = 11.84AFTKK36 pKa = 10.57NRR38 pKa = 11.84GGLKK42 pKa = 10.0SVSKK46 pKa = 10.38IQRR49 pKa = 11.84QPYY52 pKa = 8.97VPRR55 pKa = 11.84LLKK58 pKa = 9.38NTASISQLSRR68 pKa = 11.84AVKK71 pKa = 9.62GLQRR75 pKa = 11.84AKK77 pKa = 10.59LGEE80 pKa = 4.0FQTGKK85 pKa = 10.35EE86 pKa = 3.97YY87 pKa = 11.11LGFQPSTYY95 pKa = 10.44FNAQKK100 pKa = 10.53PMCFCVNDD108 pKa = 3.96FVQYY112 pKa = 8.19VTGVGVPTWTTDD124 pKa = 3.26MNDD127 pKa = 3.6GANKK131 pKa = 9.88VSNFAKK137 pKa = 10.58AEE139 pKa = 4.11KK140 pKa = 10.36NDD142 pKa = 3.87TNVSSNEE149 pKa = 3.42KK150 pKa = 10.1NYY152 pKa = 10.72YY153 pKa = 8.62NFKK156 pKa = 10.34IQDD159 pKa = 3.49NTASKK164 pKa = 10.82VVYY167 pKa = 10.02QPLSSTMTFQAHH179 pKa = 6.46ANLSVGADD187 pKa = 3.62PLYY190 pKa = 10.92VRR192 pKa = 11.84IDD194 pKa = 3.06IVRR197 pKa = 11.84QKK199 pKa = 9.56KK200 pKa = 7.23TLVNGTRR207 pKa = 11.84VLRR210 pKa = 11.84LPEE213 pKa = 4.55SIGGLGNMALKK224 pKa = 10.49DD225 pKa = 3.48PKK227 pKa = 10.74YY228 pKa = 10.31RR229 pKa = 11.84NNFNSEE235 pKa = 4.4YY236 pKa = 10.43IHH238 pKa = 6.54VLQTKK243 pKa = 9.51YY244 pKa = 11.02LYY246 pKa = 11.08LNNKK250 pKa = 8.91DD251 pKa = 3.88AGAKK255 pKa = 8.71SVRR258 pKa = 11.84SSCKK262 pKa = 9.38IHH264 pKa = 6.96VPLQGYY270 pKa = 7.97LRR272 pKa = 11.84PDD274 pKa = 3.22GDD276 pKa = 3.82ATDD279 pKa = 3.62NAGNYY284 pKa = 7.58TDD286 pKa = 3.82WYY288 pKa = 11.12NNISDD293 pKa = 4.7SKK295 pKa = 10.86KK296 pKa = 8.45IWCIMSFSDD305 pKa = 3.61TSAMSGVDD313 pKa = 3.35INIMKK318 pKa = 9.35VNRR321 pKa = 11.84WRR323 pKa = 11.84DD324 pKa = 3.38QHH326 pKa = 6.12GTDD329 pKa = 3.55

Molecular weight: 36.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1023

115

339

255.8

28.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.354 ± 0.498

1.271 ± 0.478

4.594 ± 0.528

4.399 ± 1.002

3.617 ± 0.992

7.82 ± 0.875

2.639 ± 0.657

3.812 ± 0.219

6.549 ± 1.102

8.7 ± 2.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.857 ± 0.581

5.572 ± 1.025

4.497 ± 1.253

3.519 ± 0.919

6.745 ± 0.448

7.136 ± 0.478

6.745 ± 0.27

9.384 ± 2.447

1.369 ± 0.257

3.03 ± 1.113
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski