Bacillus phage Deep Blue

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Caeruleovirus; Bacillus virus Deepblue

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 226 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A140HLW5|A0A140HLW5_9CAUD Uncharacterized protein OS=Bacillus phage Deep Blue OX=1792245 GN=Blue_154 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.04MPWEE6 pKa = 4.75CEE8 pKa = 3.99DD9 pKa = 4.32CGHH12 pKa = 6.98KK13 pKa = 10.38EE14 pKa = 3.65LASPFDD20 pKa = 3.77TFMLCPNCDD29 pKa = 3.22SQNFFHH35 pKa = 7.24GSIIEE40 pKa = 4.42DD41 pKa = 3.48DD42 pKa = 3.77VEE44 pKa = 4.11EE45 pKa = 4.13YY46 pKa = 10.94NIYY49 pKa = 10.61EE50 pKa = 4.8EE51 pKa = 5.39EE52 pKa = 4.78DD53 pKa = 3.48DD54 pKa = 4.48LNNVVIFEE62 pKa = 4.17DD63 pKa = 3.77QYY65 pKa = 11.73GEE67 pKa = 4.03KK68 pKa = 9.59LTIVNEE74 pKa = 3.86IDD76 pKa = 3.83DD77 pKa = 5.22DD78 pKa = 5.2GDD80 pKa = 3.46IHH82 pKa = 7.46LLPSDD87 pKa = 3.77RR88 pKa = 11.84NLLFSRR94 pKa = 11.84EE95 pKa = 3.96AAIEE99 pKa = 3.84LAKK102 pKa = 10.78CILKK106 pKa = 10.39VCGEE110 pKa = 4.46DD111 pKa = 3.34EE112 pKa = 4.08

Molecular weight: 12.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A140HM11|A0A140HM11_9CAUD Uncharacterized protein OS=Bacillus phage Deep Blue OX=1792245 GN=Blue_201 PE=4 SV=1
MM1 pKa = 6.78VRR3 pKa = 11.84KK4 pKa = 9.56ARR6 pKa = 11.84RR7 pKa = 11.84PRR9 pKa = 11.84LFSSNNSIKK18 pKa = 9.88TAMTNLGDD26 pKa = 4.49NLAQDD31 pKa = 4.04VLDD34 pKa = 4.25TGMKK38 pKa = 9.48TIIASKK44 pKa = 10.23PKK46 pKa = 10.21DD47 pKa = 3.22ISAKK51 pKa = 10.31RR52 pKa = 11.84MPKK55 pKa = 9.51YY56 pKa = 10.68LKK58 pKa = 9.55LTEE61 pKa = 3.97EE62 pKa = 4.21RR63 pKa = 11.84LEE65 pKa = 3.99KK66 pKa = 11.2LEE68 pKa = 4.7VIDD71 pKa = 4.97LKK73 pKa = 11.19PYY75 pKa = 9.1FAKK78 pKa = 10.44SSKK81 pKa = 10.49RR82 pKa = 11.84KK83 pKa = 6.95TKK85 pKa = 10.51KK86 pKa = 10.33DD87 pKa = 3.25GGWYY91 pKa = 8.25LTVPIRR97 pKa = 11.84RR98 pKa = 11.84KK99 pKa = 9.74ARR101 pKa = 11.84GMSRR105 pKa = 11.84RR106 pKa = 11.84MYY108 pKa = 8.73EE109 pKa = 3.45QLRR112 pKa = 11.84AVDD115 pKa = 4.43IGDD118 pKa = 3.88STSKK122 pKa = 9.85TVVSDD127 pKa = 3.18YY128 pKa = 11.0LYY130 pKa = 10.78DD131 pKa = 3.57RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84QSEE137 pKa = 4.23ASLLNYY143 pKa = 8.06TPKK146 pKa = 10.8SNNINKK152 pKa = 8.56MKK154 pKa = 10.38AGRR157 pKa = 11.84NRR159 pKa = 11.84HH160 pKa = 6.4DD161 pKa = 3.33YY162 pKa = 10.11VAFRR166 pKa = 11.84TVSDD170 pKa = 3.95KK171 pKa = 11.52SPAGSWIINRR181 pKa = 11.84GKK183 pKa = 10.52VNKK186 pKa = 9.94DD187 pKa = 3.12DD188 pKa = 3.65TSKK191 pKa = 9.88TFVANVNRR199 pKa = 11.84LMKK202 pKa = 9.97WKK204 pKa = 9.06MKK206 pKa = 10.98NGMM209 pKa = 4.0

Molecular weight: 24.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

226

0

226

46861

28

1463

207.3

23.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.065 ± 0.188

0.74 ± 0.062

6.212 ± 0.139

7.894 ± 0.292

3.954 ± 0.099

6.728 ± 0.258

1.782 ± 0.083

6.353 ± 0.132

7.763 ± 0.201

8.06 ± 0.181

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.725 ± 0.098

5.303 ± 0.156

3.135 ± 0.167

3.805 ± 0.142

4.264 ± 0.128

5.832 ± 0.135

6.481 ± 0.207

7.345 ± 0.168

1.197 ± 0.065

4.362 ± 0.13
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski