Effusibacillus lacus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Alicyclobacillaceae; Effusibacillus

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3692 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A292YLW0|A0A292YLW0_9BACL Glutamate synthase subunit alpha OS=Effusibacillus lacus OX=1348429 GN=EFBL_2570 PE=3 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84FMRR5 pKa = 11.84TIGCTLALVSLLAAPPVADD24 pKa = 4.05AQSLSIVSITVNGKK38 pKa = 7.7QVPLSDD44 pKa = 3.69SVPYY48 pKa = 10.49LDD50 pKa = 4.57SEE52 pKa = 4.74SGRR55 pKa = 11.84VMVPIRR61 pKa = 11.84FVAEE65 pKa = 3.78HH66 pKa = 6.2MGAAVNWNEE75 pKa = 4.08SAQQATIVLNSRR87 pKa = 11.84QITLTIGQKK96 pKa = 7.2TAYY99 pKa = 10.3VNWQPYY105 pKa = 7.32QLEE108 pKa = 4.29TAPVLRR114 pKa = 11.84NGRR117 pKa = 11.84TYY119 pKa = 10.77VPVRR123 pKa = 11.84FVGEE127 pKa = 3.74ALGANLYY134 pKa = 8.1WHH136 pKa = 7.36EE137 pKa = 4.09NTKK140 pKa = 9.13TVEE143 pKa = 3.8IVQNNGSSTPDD154 pKa = 3.1YY155 pKa = 10.73SDD157 pKa = 4.51PYY159 pKa = 10.58TPGTPSGDD167 pKa = 3.31QSDD170 pKa = 5.0YY171 pKa = 11.68GDD173 pKa = 3.85DD174 pKa = 3.42WDD176 pKa = 4.42TFSHH180 pKa = 6.52KK181 pKa = 9.54WDD183 pKa = 4.2EE184 pKa = 4.11YY185 pKa = 10.07SIQYY189 pKa = 9.48PEE191 pKa = 5.12DD192 pKa = 3.35WPKK195 pKa = 10.29PRR197 pKa = 11.84VVPGYY202 pKa = 10.0VKK204 pKa = 10.45WEE206 pKa = 4.21LDD208 pKa = 4.03DD209 pKa = 5.85DD210 pKa = 5.82DD211 pKa = 5.13EE212 pKa = 5.53DD213 pKa = 5.04AYY215 pKa = 10.98AYY217 pKa = 9.82IRR219 pKa = 11.84FSRR222 pKa = 11.84TTPVSNPIGEE232 pKa = 4.97DD233 pKa = 3.28DD234 pKa = 5.44ADD236 pKa = 5.17PIDD239 pKa = 5.57LNDD242 pKa = 3.95GTDD245 pKa = 3.33AEE247 pKa = 4.57YY248 pKa = 10.88EE249 pKa = 3.77KK250 pKa = 11.16DD251 pKa = 3.4EE252 pKa = 4.17YY253 pKa = 11.6SNRR256 pKa = 11.84TVYY259 pKa = 9.86MMRR262 pKa = 11.84VDD264 pKa = 3.51EE265 pKa = 4.76GDD267 pKa = 3.83YY268 pKa = 11.09EE269 pKa = 4.17ATLYY273 pKa = 11.1VSLPDD278 pKa = 5.98DD279 pKa = 3.97MDD281 pKa = 5.91DD282 pKa = 5.45DD283 pKa = 4.78YY284 pKa = 11.75DD285 pKa = 3.6IKK287 pKa = 11.54KK288 pKa = 8.78MFRR291 pKa = 11.84SFEE294 pKa = 4.08VEE296 pKa = 3.98EE297 pKa = 4.12EE298 pKa = 4.29DD299 pKa = 4.76GNEE302 pKa = 4.18DD303 pKa = 3.84LVDD306 pKa = 3.89YY307 pKa = 11.52VNTKK311 pKa = 10.47YY312 pKa = 10.47FFSLQYY318 pKa = 10.18PEE320 pKa = 4.74EE321 pKa = 4.3WDD323 pKa = 5.11DD324 pKa = 3.97GDD326 pKa = 4.72NDD328 pKa = 4.15VDD330 pKa = 3.71THH332 pKa = 7.34SNGIEE337 pKa = 3.98WDD339 pKa = 3.57LSHH342 pKa = 7.57ADD344 pKa = 3.62VEE346 pKa = 5.21FYY348 pKa = 10.68AYY350 pKa = 10.25DD351 pKa = 4.2EE352 pKa = 5.16EE353 pKa = 5.99DD354 pKa = 4.7LDD356 pKa = 4.46WDD358 pKa = 4.92DD359 pKa = 6.65LEE361 pKa = 6.94DD362 pKa = 5.14DD363 pKa = 4.18YY364 pKa = 12.24ADD366 pKa = 3.88ADD368 pKa = 3.97EE369 pKa = 4.83EE370 pKa = 4.2EE371 pKa = 5.03LRR373 pKa = 11.84DD374 pKa = 3.86GTDD377 pKa = 2.79AYY379 pKa = 11.45LWIDD383 pKa = 3.7DD384 pKa = 4.31DD385 pKa = 4.72GSYY388 pKa = 11.13VDD390 pKa = 3.81YY391 pKa = 11.62VFFFRR396 pKa = 11.84KK397 pKa = 9.8NDD399 pKa = 3.31VVYY402 pKa = 10.5KK403 pKa = 10.7GEE405 pKa = 4.02ARR407 pKa = 11.84VHH409 pKa = 6.46EE410 pKa = 5.1DD411 pKa = 3.34YY412 pKa = 10.92QDD414 pKa = 3.53EE415 pKa = 4.59YY416 pKa = 11.16EE417 pKa = 5.8DD418 pKa = 5.1EE419 pKa = 4.2ILDD422 pKa = 3.67MFKK425 pKa = 11.01SVVADD430 pKa = 3.68

Molecular weight: 49.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A292YCK6|A0A292YCK6_9BACL Branched-chain amino acid ABC transporter permease OS=Effusibacillus lacus OX=1348429 GN=EFBL_0880 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 8.69PTFQPNVRR10 pKa = 11.84KK11 pKa = 9.76RR12 pKa = 11.84KK13 pKa = 8.88KK14 pKa = 8.64VHH16 pKa = 5.57GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 10.18NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3689

3

3692

1079243

38

2767

292.3

32.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.267 ± 0.04

0.812 ± 0.013

4.941 ± 0.029

7.004 ± 0.046

3.979 ± 0.03

7.753 ± 0.039

2.084 ± 0.021

6.693 ± 0.032

5.606 ± 0.039

10.109 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.753 ± 0.02

3.537 ± 0.022

4.297 ± 0.024

3.891 ± 0.029

5.481 ± 0.033

5.497 ± 0.03

5.259 ± 0.021

7.781 ± 0.038

1.172 ± 0.02

3.084 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski