Escherichia phage JS98

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Tevenvirinae; Dhakavirus; Escherichia virus JS98

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 266 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q5QC41|Q5QC41_9CAUD Gp46 recombination endonuclease subunit OS=Escherichia phage JS98 OX=293178 GN=46 PE=4 SV=1
MM1 pKa = 7.54KK2 pKa = 9.68MYY4 pKa = 10.6KK5 pKa = 9.59HH6 pKa = 6.93DD7 pKa = 3.7FHH9 pKa = 9.27IGDD12 pKa = 3.28GWFGNVEE19 pKa = 3.93WDD21 pKa = 4.5VDD23 pKa = 4.04GSWYY27 pKa = 9.23TGTMYY32 pKa = 9.56VTSEE36 pKa = 3.99YY37 pKa = 11.05SEE39 pKa = 4.26GVSWAMEE46 pKa = 4.23LDD48 pKa = 3.77DD49 pKa = 4.94VFASSEE55 pKa = 4.01EE56 pKa = 3.8MLEE59 pKa = 4.11YY60 pKa = 10.87AEE62 pKa = 4.02QTIRR66 pKa = 11.84EE67 pKa = 4.28NNTT70 pKa = 2.83

Molecular weight: 8.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A8R9Q6|A8R9Q6_9CAUD Nucleoid disruption protein OS=Escherichia phage JS98 OX=293178 GN=ndd PE=4 SV=1
MM1 pKa = 7.56SNFHH5 pKa = 6.63NEE7 pKa = 4.71HH8 pKa = 4.57VMQWHH13 pKa = 5.93RR14 pKa = 11.84HH15 pKa = 4.07NLKK18 pKa = 9.03LTGVFGRR25 pKa = 11.84PP26 pKa = 3.12

Molecular weight: 3.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

266

0

266

52266

26

1349

196.5

22.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.133 ± 0.166

1.047 ± 0.066

6.515 ± 0.105

7.297 ± 0.19

4.358 ± 0.101

6.272 ± 0.223

1.684 ± 0.069

6.846 ± 0.115

7.406 ± 0.199

7.623 ± 0.156

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.769 ± 0.102

5.348 ± 0.119

3.601 ± 0.1

3.452 ± 0.088

4.303 ± 0.101

6.09 ± 0.137

5.841 ± 0.218

6.698 ± 0.105

1.427 ± 0.072

4.292 ± 0.122
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski