Ralstonia phage RSM1

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Habenivirus; Ralstonia virus RSM1

Average proteome isoelectric point is 7.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0JC15|A0JC15_9VIRU Zot domain-containing protein OS=Ralstonia phage RSM1 OX=384359 PE=4 SV=1
MM1 pKa = 7.52FDD3 pKa = 3.65AVINALSALAQWLDD17 pKa = 3.95GIFVAIFAALWQITEE32 pKa = 4.38DD33 pKa = 4.28LFIDD37 pKa = 4.43SLDD40 pKa = 4.01LLLQGVTAVLGTLPAPTFLSGVSLQSAFNSLGGDD74 pKa = 2.86ILFFFGVFNIGQGIGLLGGRR94 pKa = 11.84SRR96 pKa = 11.84FVWRR100 pKa = 11.84ARR102 pKa = 11.84SS103 pKa = 3.23

Molecular weight: 11.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0JC10|A0JC10_9VIRU Uncharacterized protein OS=Ralstonia phage RSM1 OX=384359 PE=4 SV=1
MM1 pKa = 7.42KK2 pKa = 10.58LSTPLLLLLVGWLATAVAHH21 pKa = 5.67NAWRR25 pKa = 11.84LFSVIGFAGVSMLIQGGHH43 pKa = 4.72GVARR47 pKa = 11.84VCLLLAVVLVVVRR60 pKa = 11.84LVRR63 pKa = 11.84RR64 pKa = 11.84FFRR67 pKa = 11.84RR68 pKa = 11.84AA69 pKa = 2.88

Molecular weight: 7.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

2489

46

498

165.9

18.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.897 ± 0.65

1.245 ± 0.283

4.419 ± 0.281

4.018 ± 0.985

3.254 ± 0.746

8.196 ± 1.113

1.928 ± 0.434

3.978 ± 0.343

4.379 ± 0.637

7.352 ± 0.949

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.4

2.893 ± 0.374

4.259 ± 0.715

4.701 ± 0.84

7.031 ± 1.367

6.227 ± 0.638

6.83 ± 1.256

9.16 ± 0.896

2.451 ± 0.362

2.25 ± 0.323
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski