Amino acid dipepetide frequency for Nitratireductor pacificus pht-3B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	18.068AlaAla: 18.068 ± 0.19	1.048AlaCys: 1.048 ± 0.028	6.949AlaAsp: 6.949 ± 0.073	8.349AlaGlu: 8.349 ± 0.093	4.993AlaPhe: 4.993 ± 0.071	12.056AlaGly: 12.056 ± 0.128	2.323AlaHis: 2.323 ± 0.046	6.576AlaIle: 6.576 ± 0.072	3.442AlaLys: 3.442 ± 0.065	14.533AlaLeu: 14.533 ± 0.138	3.809AlaMet: 3.809 ± 0.06	2.77AlaAsn: 2.77 ± 0.046	5.573AlaPro: 5.573 ± 0.079	3.554AlaGln: 3.554 ± 0.055	9.52AlaArg: 9.52 ± 0.101	6.297AlaSer: 6.297 ± 0.077	5.906AlaThr: 5.906 ± 0.07	9.176AlaVal: 9.176 ± 0.091	1.467AlaTrp: 1.467 ± 0.039	2.646AlaTyr: 2.646 ± 0.048	0.0AlaXaa: 0.0 ± 0.0
Cys	0.974CysAla: 0.974 ± 0.029	0.089CysCys: 0.089 ± 0.008	0.453CysAsp: 0.453 ± 0.02	0.396CysGlu: 0.396 ± 0.017	0.326CysPhe: 0.326 ± 0.015	0.926CysGly: 0.926 ± 0.027	0.234CysHis: 0.234 ± 0.014	0.366CysIle: 0.366 ± 0.017	0.149CysLys: 0.149 ± 0.011	0.738CysLeu: 0.738 ± 0.023	0.155CysMet: 0.155 ± 0.011	0.177CysAsn: 0.177 ± 0.011	0.406CysPro: 0.406 ± 0.021	0.204CysGln: 0.204 ± 0.012	0.649CysArg: 0.649 ± 0.026	0.447CysSer: 0.447 ± 0.02	0.391CysThr: 0.391 ± 0.021	0.575CysVal: 0.575 ± 0.022	0.115CysTrp: 0.115 ± 0.01	0.193CysTyr: 0.193 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.224AspAla: 7.224 ± 0.088	0.477AspCys: 0.477 ± 0.024	3.111AspAsp: 3.111 ± 0.062	3.577AspGlu: 3.577 ± 0.055	2.245AspPhe: 2.245 ± 0.041	5.417AspGly: 5.417 ± 0.072	1.254AspHis: 1.254 ± 0.036	3.04AspIle: 3.04 ± 0.05	1.533AspLys: 1.533 ± 0.038	5.677AspLeu: 5.677 ± 0.07	1.515AspMet: 1.515 ± 0.034	1.263AspAsn: 1.263 ± 0.036	3.353AspPro: 3.353 ± 0.053	1.494AspGln: 1.494 ± 0.028	4.327AspArg: 4.327 ± 0.061	1.889AspSer: 1.889 ± 0.038	2.543AspThr: 2.543 ± 0.043	3.988AspVal: 3.988 ± 0.062	0.931AspTrp: 0.931 ± 0.033	1.443AspTyr: 1.443 ± 0.036	0.0AspXaa: 0.0 ± 0.0
Glu	8.632GluAla: 8.632 ± 0.096	0.354GluCys: 0.354 ± 0.018	2.977GluAsp: 2.977 ± 0.05	3.627GluGlu: 3.627 ± 0.067	1.73GluPhe: 1.73 ± 0.036	4.743GluGly: 4.743 ± 0.065	1.198GluHis: 1.198 ± 0.034	3.597GluIle: 3.597 ± 0.052	2.541GluLys: 2.541 ± 0.052	5.143GluLeu: 5.143 ± 0.063	1.674GluMet: 1.674 ± 0.035	1.749GluAsn: 1.749 ± 0.039	2.808GluPro: 2.808 ± 0.055	1.998GluGln: 1.998 ± 0.036	5.367GluArg: 5.367 ± 0.078	2.234GluSer: 2.234 ± 0.04	4.158GluThr: 4.158 ± 0.06	3.6GluVal: 3.6 ± 0.05	0.659GluTrp: 0.659 ± 0.023	0.927GluTyr: 0.927 ± 0.03	0.0GluXaa: 0.0 ± 0.0
Phe	4.891PheAla: 4.891 ± 0.068	0.369PheCys: 0.369 ± 0.018	2.718PheAsp: 2.718 ± 0.048	2.261PheGlu: 2.261 ± 0.041	1.553PhePhe: 1.553 ± 0.038	3.852PheGly: 3.852 ± 0.059	0.802PheHis: 0.802 ± 0.024	1.831PheIle: 1.831 ± 0.044	0.869PheLys: 0.869 ± 0.027	3.715PheLeu: 3.715 ± 0.063	0.932PheMet: 0.932 ± 0.025	1.028PheAsn: 1.028 ± 0.031	1.702PhePro: 1.702 ± 0.039	1.033PheGln: 1.033 ± 0.03	2.359PheArg: 2.359 ± 0.042	2.439PheSer: 2.439 ± 0.046	1.984PheThr: 1.984 ± 0.041	2.946PheVal: 2.946 ± 0.056	0.558PheTrp: 0.558 ± 0.022	0.923PheTyr: 0.923 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.217GlyAla: 10.217 ± 0.101	0.814GlyCys: 0.814 ± 0.027	4.574GlyAsp: 4.574 ± 0.066	5.295GlyGlu: 5.295 ± 0.069	3.944GlyPhe: 3.944 ± 0.056	7.856GlyGly: 7.856 ± 0.104	1.935GlyHis: 1.935 ± 0.04	4.939GlyIle: 4.939 ± 0.065	3.114GlyLys: 3.114 ± 0.057	9.304GlyLeu: 9.304 ± 0.099	2.626GlyMet: 2.626 ± 0.049	2.197GlyAsn: 2.197 ± 0.046	3.43GlyPro: 3.43 ± 0.048	2.754GlyGln: 2.754 ± 0.045	6.593GlyArg: 6.593 ± 0.073	4.678GlySer: 4.678 ± 0.08	4.68GlyThr: 4.68 ± 0.074	6.326GlyVal: 6.326 ± 0.076	1.349GlyTrp: 1.349 ± 0.032	2.333GlyTyr: 2.333 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	2.376HisAla: 2.376 ± 0.045	0.237HisCys: 0.237 ± 0.015	1.2HisAsp: 1.2 ± 0.032	1.069HisGlu: 1.069 ± 0.029	0.896HisPhe: 0.896 ± 0.026	1.956HisGly: 1.956 ± 0.04	0.532HisHis: 0.532 ± 0.029	0.907HisIle: 0.907 ± 0.029	0.43HisLys: 0.43 ± 0.018	2.083HisLeu: 2.083 ± 0.041	0.508HisMet: 0.508 ± 0.021	0.439HisAsn: 0.439 ± 0.017	1.255HisPro: 1.255 ± 0.036	0.57HisGln: 0.57 ± 0.026	1.458HisArg: 1.458 ± 0.035	0.883HisSer: 0.883 ± 0.027	0.795HisThr: 0.795 ± 0.026	1.567HisVal: 1.567 ± 0.033	0.306HisTrp: 0.306 ± 0.016	0.565HisTyr: 0.565 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	7.766IleAla: 7.766 ± 0.087	0.49IleCys: 0.49 ± 0.019	3.657IleAsp: 3.657 ± 0.052	3.533IleGlu: 3.533 ± 0.051	1.706IlePhe: 1.706 ± 0.043	5.169IleGly: 5.169 ± 0.061	0.894IleHis: 0.894 ± 0.026	2.34IleIle: 2.34 ± 0.046	1.157IleLys: 1.157 ± 0.035	4.703IleLeu: 4.703 ± 0.062	1.118IleMet: 1.118 ± 0.03	1.314IleAsn: 1.314 ± 0.033	2.222IlePro: 2.222 ± 0.043	1.116IleGln: 1.116 ± 0.034	3.269IleArg: 3.269 ± 0.047	2.829IleSer: 2.829 ± 0.042	2.555IleThr: 2.555 ± 0.05	4.465IleVal: 4.465 ± 0.066	0.578IleTrp: 0.578 ± 0.023	1.091IleTyr: 1.091 ± 0.026	0.0IleXaa: 0.0 ± 0.0
Lys	4.059LysAla: 4.059 ± 0.064	0.131LysCys: 0.131 ± 0.01	1.492LysAsp: 1.492 ± 0.04	1.652LysGlu: 1.652 ± 0.041	0.746LysPhe: 0.746 ± 0.026	2.486LysGly: 2.486 ± 0.055	0.551LysHis: 0.551 ± 0.019	1.56LysIle: 1.56 ± 0.038	1.248LysLys: 1.248 ± 0.04	2.973LysLeu: 2.973 ± 0.058	0.695LysMet: 0.695 ± 0.024	0.828LysAsn: 0.828 ± 0.027	1.977LysPro: 1.977 ± 0.039	0.897LysGln: 0.897 ± 0.027	2.226LysArg: 2.226 ± 0.042	1.621LysSer: 1.621 ± 0.037	1.862LysThr: 1.862 ± 0.041	2.122LysVal: 2.122 ± 0.047	0.346LysTrp: 0.346 ± 0.017	0.543LysTyr: 0.543 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	13.996LeuAla: 13.996 ± 0.132	0.88LeuCys: 0.88 ± 0.025	6.063LeuAsp: 6.063 ± 0.071	5.555LeuGlu: 5.555 ± 0.076	3.931LeuPhe: 3.931 ± 0.067	8.534LeuGly: 8.534 ± 0.092	1.775LeuHis: 1.775 ± 0.04	5.198LeuIle: 5.198 ± 0.074	3.521LeuLys: 3.521 ± 0.054	9.718LeuLeu: 9.718 ± 0.129	2.44LeuMet: 2.44 ± 0.049	2.443LeuAsn: 2.443 ± 0.042	5.545LeuPro: 5.545 ± 0.066	2.632LeuGln: 2.632 ± 0.048	6.658LeuArg: 6.658 ± 0.087	6.709LeuSer: 6.709 ± 0.083	5.413LeuThr: 5.413 ± 0.067	7.69LeuVal: 7.69 ± 0.094	1.109LeuTrp: 1.109 ± 0.028	2.145LeuTyr: 2.145 ± 0.036	0.0LeuXaa: 0.0 ± 0.0
Met	3.392MetAla: 3.392 ± 0.054	0.146MetCys: 0.146 ± 0.011	1.177MetAsp: 1.177 ± 0.028	1.279MetGlu: 1.279 ± 0.032	0.75MetPhe: 0.75 ± 0.027	1.922MetGly: 1.922 ± 0.04	0.457MetHis: 0.457 ± 0.021	1.468MetIle: 1.468 ± 0.04	1.013MetLys: 1.013 ± 0.027	2.698MetLeu: 2.698 ± 0.046	0.714MetMet: 0.714 ± 0.021	0.843MetAsn: 0.843 ± 0.026	1.567MetPro: 1.567 ± 0.038	0.928MetGln: 0.928 ± 0.022	2.116MetArg: 2.116 ± 0.042	1.616MetSer: 1.616 ± 0.031	1.924MetThr: 1.924 ± 0.037	1.832MetVal: 1.832 ± 0.037	0.217MetTrp: 0.217 ± 0.015	0.316MetTyr: 0.316 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	3.212AsnAla: 3.212 ± 0.047	0.22AsnCys: 0.22 ± 0.013	1.353AsnAsp: 1.353 ± 0.033	1.271AsnGlu: 1.271 ± 0.033	0.927AsnPhe: 0.927 ± 0.028	2.412AsnGly: 2.412 ± 0.053	0.484AsnHis: 0.484 ± 0.02	1.288AsnIle: 1.288 ± 0.035	0.591AsnLys: 0.591 ± 0.021	2.464AsnLeu: 2.464 ± 0.046	0.578AsnMet: 0.578 ± 0.021	0.644AsnAsn: 0.644 ± 0.026	1.806AsnPro: 1.806 ± 0.038	0.67AsnGln: 0.67 ± 0.023	1.794AsnArg: 1.794 ± 0.04	1.091AsnSer: 1.091 ± 0.032	1.184AsnThr: 1.184 ± 0.032	1.813AsnVal: 1.813 ± 0.044	0.375AsnTrp: 0.375 ± 0.017	0.626AsnTyr: 0.626 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	6.322ProAla: 6.322 ± 0.087	0.295ProCys: 0.295 ± 0.015	3.467ProAsp: 3.467 ± 0.049	3.883ProGlu: 3.883 ± 0.061	2.126ProPhe: 2.126 ± 0.04	4.62ProGly: 4.62 ± 0.056	1.059ProHis: 1.059 ± 0.028	2.132ProIle: 2.132 ± 0.041	1.417ProLys: 1.417 ± 0.036	4.781ProLeu: 4.781 ± 0.056	1.176ProMet: 1.176 ± 0.032	1.15ProAsn: 1.15 ± 0.034	2.308ProPro: 2.308 ± 0.05	1.597ProGln: 1.597 ± 0.037	2.96ProArg: 2.96 ± 0.055	2.612ProSer: 2.612 ± 0.04	2.136ProThr: 2.136 ± 0.041	4.342ProVal: 4.342 ± 0.069	0.626ProTrp: 0.626 ± 0.021	1.189ProTyr: 1.189 ± 0.031	0.0ProXaa: 0.0 ± 0.0
Gln	3.933GlnAla: 3.933 ± 0.061	0.194GlnCys: 0.194 ± 0.01	1.401GlnAsp: 1.401 ± 0.033	1.589GlnGlu: 1.589 ± 0.038	0.934GlnPhe: 0.934 ± 0.027	2.198GlnGly: 2.198 ± 0.044	0.586GlnHis: 0.586 ± 0.022	1.606GlnIle: 1.606 ± 0.041	1.094GlnLys: 1.094 ± 0.034	2.528GlnLeu: 2.528 ± 0.047	0.904GlnMet: 0.904 ± 0.025	0.804GlnAsn: 0.804 ± 0.025	1.634GlnPro: 1.634 ± 0.036	1.022GlnGln: 1.022 ± 0.034	2.269GlnArg: 2.269 ± 0.044	1.582GlnSer: 1.582 ± 0.033	1.593GlnThr: 1.593 ± 0.031	2.145GlnVal: 2.145 ± 0.038	0.339GlnTrp: 0.339 ± 0.015	0.55GlnTyr: 0.55 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	8.431ArgAla: 8.431 ± 0.087	0.46ArgCys: 0.46 ± 0.022	4.202ArgAsp: 4.202 ± 0.06	4.314ArgGlu: 4.314 ± 0.063	3.218ArgPhe: 3.218 ± 0.056	4.923ArgGly: 4.923 ± 0.068	1.79ArgHis: 1.79 ± 0.037	4.176ArgIle: 4.176 ± 0.049	2.266ArgLys: 2.266 ± 0.048	8.244ArgLeu: 8.244 ± 0.089	2.028ArgMet: 2.028 ± 0.041	1.88ArgAsn: 1.88 ± 0.034	3.495ArgPro: 3.495 ± 0.057	2.588ArgGln: 2.588 ± 0.045	5.909ArgArg: 5.909 ± 0.087	3.611ArgSer: 3.611 ± 0.056	3.347ArgThr: 3.347 ± 0.055	4.835ArgVal: 4.835 ± 0.077	0.88ArgTrp: 0.88 ± 0.025	1.636ArgTyr: 1.636 ± 0.039	0.0ArgXaa: 0.0 ± 0.0
Ser	6.279SerAla: 6.279 ± 0.072	0.374SerCys: 0.374 ± 0.018	2.841SerAsp: 2.841 ± 0.046	2.835SerGlu: 2.835 ± 0.051	2.271SerPhe: 2.271 ± 0.041	5.919SerGly: 5.919 ± 0.08	0.99SerHis: 0.99 ± 0.028	2.738SerIle: 2.738 ± 0.044	1.339SerLys: 1.339 ± 0.032	5.355SerLeu: 5.355 ± 0.071	1.362SerMet: 1.362 ± 0.031	1.253SerAsn: 1.253 ± 0.039	2.468SerPro: 2.468 ± 0.046	1.465SerGln: 1.465 ± 0.031	3.499SerArg: 3.499 ± 0.052	2.708SerSer: 2.708 ± 0.056	2.509SerThr: 2.509 ± 0.049	4.107SerVal: 4.107 ± 0.059	0.686SerTrp: 0.686 ± 0.024	1.285SerTyr: 1.285 ± 0.031	0.0SerXaa: 0.0 ± 0.0
Thr	6.318ThrAla: 6.318 ± 0.065	0.392ThrCys: 0.392 ± 0.017	2.604ThrAsp: 2.604 ± 0.045	2.682ThrGlu: 2.682 ± 0.044	2.023ThrPhe: 2.023 ± 0.042	5.344ThrGly: 5.344 ± 0.078	1.01ThrHis: 1.01 ± 0.029	2.88ThrIle: 2.88 ± 0.056	1.185ThrLys: 1.185 ± 0.031	5.757ThrLeu: 5.757 ± 0.075	1.279ThrMet: 1.279 ± 0.032	1.141ThrAsn: 1.141 ± 0.033	3.153ThrPro: 3.153 ± 0.045	1.265ThrGln: 1.265 ± 0.032	3.383ThrArg: 3.383 ± 0.062	2.516ThrSer: 2.516 ± 0.048	2.558ThrThr: 2.558 ± 0.049	4.581ThrVal: 4.581 ± 0.057	0.58ThrTrp: 0.58 ± 0.023	1.131ThrTyr: 1.131 ± 0.035	0.0ThrXaa: 0.0 ± 0.0
Val	9.282ValAla: 9.282 ± 0.103	0.639ValCys: 0.639 ± 0.024	4.078ValAsp: 4.078 ± 0.065	4.75ValGlu: 4.75 ± 0.062	3.059ValPhe: 3.059 ± 0.058	5.663ValGly: 5.663 ± 0.068	1.392ValHis: 1.392 ± 0.034	3.901ValIle: 3.901 ± 0.063	2.098ValLys: 2.098 ± 0.043	7.732ValLeu: 7.732 ± 0.091	2.032ValMet: 2.032 ± 0.041	1.878ValAsn: 1.878 ± 0.037	3.778ValPro: 3.778 ± 0.06	1.871ValGln: 1.871 ± 0.042	4.949ValArg: 4.949 ± 0.066	4.625ValSer: 4.625 ± 0.055	4.395ValThr: 4.395 ± 0.052	5.867ValVal: 5.867 ± 0.085	0.836ValTrp: 0.836 ± 0.029	1.503ValTyr: 1.503 ± 0.032	0.0ValXaa: 0.0 ± 0.0
Trp	1.122TrpAla: 1.122 ± 0.029	0.129TrpCys: 0.129 ± 0.01	0.598TrpAsp: 0.598 ± 0.025	0.552TrpGlu: 0.552 ± 0.02	0.512TrpPhe: 0.512 ± 0.021	0.827TrpGly: 0.827 ± 0.025	0.3TrpHis: 0.3 ± 0.015	0.601TrpIle: 0.601 ± 0.02	0.444TrpLys: 0.444 ± 0.017	1.566TrpLeu: 1.566 ± 0.041	0.36TrpMet: 0.36 ± 0.015	0.437TrpAsn: 0.437 ± 0.019	0.673TrpPro: 0.673 ± 0.021	0.54TrpGln: 0.54 ± 0.02	1.11TrpArg: 1.11 ± 0.032	0.765TrpSer: 0.765 ± 0.026	0.699TrpThr: 0.699 ± 0.023	0.765TrpVal: 0.765 ± 0.03	0.206TrpTrp: 0.206 ± 0.014	0.265TrpTyr: 0.265 ± 0.016	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.56TyrAla: 2.56 ± 0.044	0.228TyrCys: 0.228 ± 0.012	1.387TyrAsp: 1.387 ± 0.036	1.25TyrGlu: 1.25 ± 0.034	0.877TyrPhe: 0.877 ± 0.025	2.136TyrGly: 2.136 ± 0.047	0.465TyrHis: 0.465 ± 0.02	0.827TyrIle: 0.827 ± 0.026	0.563TyrLys: 0.563 ± 0.022	2.242TyrLeu: 2.242 ± 0.048	0.469TyrMet: 0.469 ± 0.018	0.565TyrAsn: 0.565 ± 0.022	1.046TyrPro: 1.046 ± 0.025	0.707TyrGln: 0.707 ± 0.026	1.815TyrArg: 1.815 ± 0.036	1.129TyrSer: 1.129 ± 0.027	1.15TyrThr: 1.15 ± 0.032	1.586TyrVal: 1.586 ± 0.032	0.309TyrTrp: 0.309 ± 0.015	0.579TyrTyr: 0.579 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4196 proteins (1318631 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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