Thrush coronavirus HKU12 (isolate Grey-backed thrush/Hong Kong/HKU12-600/2007) (ThCoV-HKU12) (Thrush coronavirus HKU12-600)

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Deltacoronavirus; Buldecovirus; unclassified Buldecovirus

Average proteome isoelectric point is 6.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6VDY2|B6VDY2_THCOV Nucleocapsid phosphoprotein OS=Thrush coronavirus HKU12 (isolate Grey-backed thrush/Hong Kong/HKU12-600/2007) OX=572290 GN=N PE=4 SV=1
MM1 pKa = 7.65CNCLYY6 pKa = 10.09QVKK9 pKa = 10.51ALVEE13 pKa = 3.95YY14 pKa = 10.78SKK16 pKa = 8.81THH18 pKa = 5.53GKK20 pKa = 8.37TDD22 pKa = 3.44VLEE25 pKa = 4.48LLDD28 pKa = 4.37PLVKK32 pKa = 9.74TRR34 pKa = 11.84CFAYY38 pKa = 9.76TLVVCINANPVAFSILPRR56 pKa = 11.84KK57 pKa = 9.77LLINGEE63 pKa = 4.24PLLIEE68 pKa = 4.16YY69 pKa = 10.77GNVYY73 pKa = 10.57GKK75 pKa = 10.48DD76 pKa = 3.67FIIRR80 pKa = 11.84PSLQVILEE88 pKa = 4.3EE89 pKa = 4.36EE90 pKa = 4.55CC91 pKa = 4.25

Molecular weight: 10.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6VDY5|B6VDY5_THCOV Nonstructural protein OS=Thrush coronavirus HKU12 (isolate Grey-backed thrush/Hong Kong/HKU12-600/2007) OX=572290 GN=NS7c PE=4 SV=1
MM1 pKa = 7.13GTSQSSVSVNNQITNIHH18 pKa = 6.13ASEE21 pKa = 4.34HH22 pKa = 6.58SSVQTSNSNTSEE34 pKa = 3.8IQALLQQGLPYY45 pKa = 10.64LVILLSILLLLQSAFIIHH63 pKa = 6.89LLRR66 pKa = 11.84RR67 pKa = 11.84VRR69 pKa = 11.84RR70 pKa = 11.84LKK72 pKa = 10.84SRR74 pKa = 11.84LFAAAPLLTT83 pKa = 4.3

Molecular weight: 9.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

8494

77

6286

943.8

105.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.264 ± 0.355

2.731 ± 0.382

5.321 ± 0.513

3.944 ± 0.341

4.297 ± 0.344

5.133 ± 0.246

2.508 ± 0.308

6.228 ± 0.525

5.027 ± 0.569

9.336 ± 0.405

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.707 ± 0.197

5.498 ± 0.409

4.721 ± 0.563

4.568 ± 0.837

3.603 ± 0.398

6.346 ± 0.639

7.417 ± 0.403

8.429 ± 0.761

0.942 ± 0.352

4.98 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski