Streptomyces variegatus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae;

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7371 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0M2GFD2|A0A0M2GFD2_9ACTN NADH-quinone oxidoreductase subunit B OS=Streptomyces variegatus OX=284040 GN=nuoB PE=3 SV=1
MM1 pKa = 7.58SVQQVAGADD10 pKa = 3.79GAEE13 pKa = 3.99LEE15 pKa = 4.34VWIDD19 pKa = 3.38QDD21 pKa = 3.99LCTGDD26 pKa = 5.01GICAQYY32 pKa = 10.9APEE35 pKa = 4.23VFEE38 pKa = 5.9LDD40 pKa = 3.05IDD42 pKa = 3.63GLAYY46 pKa = 10.42VKK48 pKa = 10.8SADD51 pKa = 5.3DD52 pKa = 4.73EE53 pKa = 4.41LLQDD57 pKa = 4.41KK58 pKa = 10.51GATTPVPLPLLTDD71 pKa = 4.3VIDD74 pKa = 4.0SAKK77 pKa = 9.8EE78 pKa = 3.88CPGEE82 pKa = 4.38CIHH85 pKa = 6.24VRR87 pKa = 11.84RR88 pKa = 11.84VSDD91 pKa = 3.25RR92 pKa = 11.84AEE94 pKa = 3.95VYY96 pKa = 10.9GPDD99 pKa = 3.42VEE101 pKa = 4.49

Molecular weight: 10.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0M2GPS7|A0A0M2GPS7_9ACTN Branched-chain amino acid ABC transporter permease OS=Streptomyces variegatus OX=284040 GN=UK15_23045 PE=4 SV=1
MM1 pKa = 7.57PARR4 pKa = 11.84VPVAVAPAAVVPVAAVVPVAAVAVARR30 pKa = 11.84VAAASPVVPVAAVAVSPVVPVVPVAVAAVSPVVPVVPAAVAVPASVAVPAVPVAVVARR88 pKa = 11.84RR89 pKa = 11.84VRR91 pKa = 11.84SAVPAVRR98 pKa = 11.84RR99 pKa = 11.84VVAASRR105 pKa = 11.84SGRR108 pKa = 11.84GARR111 pKa = 11.84STRR114 pKa = 11.84PCRR117 pKa = 11.84PRR119 pKa = 11.84RR120 pKa = 11.84SAVV123 pKa = 2.97

Molecular weight: 11.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7371

0

7371

2399847

27

3642

325.6

34.91

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.318 ± 0.039

0.777 ± 0.007

6.02 ± 0.023

5.75 ± 0.03

2.771 ± 0.017

9.501 ± 0.029

2.315 ± 0.015

3.18 ± 0.018

2.265 ± 0.024

10.33 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.767 ± 0.011

1.786 ± 0.016

6.055 ± 0.025

2.8 ± 0.016

7.975 ± 0.031

5.036 ± 0.021

6.166 ± 0.024

8.511 ± 0.028

1.547 ± 0.012

2.13 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski