Saccharopolyspora spinosa

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria;

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7300 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N3XW49|A0A2N3XW49_SACSN Uncharacterized protein OS=Saccharopolyspora spinosa OX=60894 GN=A8926_2481 PE=4 SV=1
MM1 pKa = 7.18SWSIEE6 pKa = 3.84IDD8 pKa = 3.06ADD10 pKa = 3.84TCIGSGMCAGVAPDD24 pKa = 3.89HH25 pKa = 6.73FEE27 pKa = 4.44LVDD30 pKa = 4.47GYY32 pKa = 11.6SCAKK36 pKa = 9.38KK37 pKa = 9.72ATVEE41 pKa = 4.08PTDD44 pKa = 3.65EE45 pKa = 4.23VVDD48 pKa = 3.86AAEE51 pKa = 4.34SCPVEE56 pKa = 4.8AILVKK61 pKa = 10.64DD62 pKa = 4.18AEE64 pKa = 4.5TGAAIAPEE72 pKa = 4.15KK73 pKa = 10.79

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N3XS48|A0A2N3XS48_SACSN Cell elongation-specific peptidoglycan biosynthesis regulator RodA OS=Saccharopolyspora spinosa OX=60894 GN=A8926_1043 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.53GKK5 pKa = 8.66RR6 pKa = 11.84TFQPNNRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84ARR17 pKa = 11.84KK18 pKa = 8.65HH19 pKa = 4.47GFRR22 pKa = 11.84LRR24 pKa = 11.84MRR26 pKa = 11.84TRR28 pKa = 11.84AGRR31 pKa = 11.84AIVASRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84GRR42 pKa = 11.84AQLTAA47 pKa = 4.03

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7300

0

7300

2427316

29

14043

332.5

35.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.88 ± 0.043

0.839 ± 0.014

5.983 ± 0.028

6.103 ± 0.059

2.845 ± 0.019

9.157 ± 0.049

2.261 ± 0.02

3.529 ± 0.041

2.257 ± 0.043

10.17 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.75 ± 0.016

2.003 ± 0.018

5.794 ± 0.029

3.324 ± 0.034

8.047 ± 0.033

5.333 ± 0.027

5.543 ± 0.039

8.719 ± 0.039

1.573 ± 0.017

1.892 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski