Pseudooceanicola algae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudooceanicola

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3817 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A418SFN5|A0A418SFN5_9RHOB Histidine kinase OS=Pseudooceanicola algae OX=1537215 GN=regB PE=4 SV=1
MM1 pKa = 7.56GPLGWVTDD9 pKa = 4.03RR10 pKa = 11.84YY11 pKa = 8.97GAQMGKK17 pKa = 9.57NDD19 pKa = 4.07AMMMHH24 pKa = 6.92RR25 pKa = 11.84VFGGLTALTLTLLASAAIACPDD47 pKa = 3.49VEE49 pKa = 4.8EE50 pKa = 5.72DD51 pKa = 5.58GEE53 pKa = 4.2DD54 pKa = 3.47LYY56 pKa = 11.87YY57 pKa = 11.15SSDD60 pKa = 3.9DD61 pKa = 3.22LWNAVAFAVIAGGDD75 pKa = 3.55QDD77 pKa = 6.14LDD79 pKa = 3.49RR80 pKa = 11.84CDD82 pKa = 4.04FNGPFTTGHH91 pKa = 5.41VAAPPDD97 pKa = 3.81FEE99 pKa = 4.12LHH101 pKa = 5.06YY102 pKa = 10.8RR103 pKa = 11.84KK104 pKa = 7.87TRR106 pKa = 11.84DD107 pKa = 2.82FDD109 pKa = 4.09LRR111 pKa = 11.84FTAVGDD117 pKa = 3.89CDD119 pKa = 3.81TVLLINTGAGNWFFDD134 pKa = 5.07DD135 pKa = 6.5DD136 pKa = 4.52DD137 pKa = 4.43GQYY140 pKa = 10.95DD141 pKa = 3.94AQIHH145 pKa = 5.32LTNPSDD151 pKa = 3.43GLYY154 pKa = 10.3DD155 pKa = 3.42IWIGTYY161 pKa = 7.51EE162 pKa = 4.01NRR164 pKa = 11.84NCEE167 pKa = 3.79ATLTIEE173 pKa = 4.39TFPEE177 pKa = 4.41DD178 pKa = 3.17SS179 pKa = 3.57

Molecular weight: 19.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A418SKL8|A0A418SKL8_9RHOB Pseudouridine synthase OS=Pseudooceanicola algae OX=1537215 GN=rluC PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 10.45AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3817

0

3817

1216675

29

2782

318.8

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.402 ± 0.058

0.892 ± 0.013

6.114 ± 0.034

5.723 ± 0.039

3.612 ± 0.025

8.88 ± 0.038

1.983 ± 0.019

5.119 ± 0.027

2.764 ± 0.034

10.449 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.828 ± 0.021

2.401 ± 0.021

5.357 ± 0.029

3.264 ± 0.021

6.842 ± 0.04

5.322 ± 0.025

5.509 ± 0.026

7.016 ± 0.032

1.366 ± 0.017

2.157 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski