Enterococcus phage Entf1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saphexavirus; unclassified Saphexavirus

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 96 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5TMH9|A0A4Y5TMH9_9CAUD Putative homing endonuclease OS=Enterococcus phage Entf1 OX=2583507 PE=4 SV=1
MM1 pKa = 7.23TKK3 pKa = 10.29EE4 pKa = 3.91NNVFLDD10 pKa = 3.39EE11 pKa = 4.63KK12 pKa = 11.21GFFEE16 pKa = 4.52TVIEE20 pKa = 4.01ALEE23 pKa = 4.31NGFSEE28 pKa = 5.38YY29 pKa = 11.25YY30 pKa = 10.28CDD32 pKa = 3.83LQSEE36 pKa = 4.63VFDD39 pKa = 4.76CGVNADD45 pKa = 3.51IKK47 pKa = 11.03DD48 pKa = 3.67LEE50 pKa = 4.41EE51 pKa = 4.26YY52 pKa = 10.96GIFDD56 pKa = 5.61AIGEE60 pKa = 4.16IQEE63 pKa = 4.22YY64 pKa = 8.74EE65 pKa = 3.98SNYY68 pKa = 10.16FGEE71 pKa = 4.13ISIDD75 pKa = 3.58LSSPDD80 pKa = 3.25NVANMLYY87 pKa = 10.09FIKK90 pKa = 10.38GHH92 pKa = 5.46EE93 pKa = 4.17FLYY96 pKa = 9.48EE97 pKa = 3.87TLNFNTILEE106 pKa = 4.38EE107 pKa = 3.92VSEE110 pKa = 4.57DD111 pKa = 3.61FFGNTDD117 pKa = 3.18LWNEE121 pKa = 4.05QAKK124 pKa = 10.52EE125 pKa = 4.12EE126 pKa = 4.13YY127 pKa = 10.27NKK129 pKa = 10.92AIVKK133 pKa = 10.25RR134 pKa = 11.84LMEE137 pKa = 4.15EE138 pKa = 4.32FKK140 pKa = 11.08NVYY143 pKa = 9.44CC144 pKa = 5.52

Molecular weight: 16.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5TPH6|A0A4Y5TPH6_9CAUD Tail fiber protein OS=Enterococcus phage Entf1 OX=2583507 PE=4 SV=1
MM1 pKa = 7.1NVEE4 pKa = 4.22SRR6 pKa = 11.84NSEE9 pKa = 4.24KK10 pKa = 10.57AWNRR14 pKa = 11.84AIVKK18 pKa = 10.21AEE20 pKa = 3.61QEE22 pKa = 4.03AAKK25 pKa = 10.69KK26 pKa = 10.45KK27 pKa = 8.64MEE29 pKa = 4.46RR30 pKa = 11.84LAKK33 pKa = 9.46MRR35 pKa = 11.84AKK37 pKa = 10.17SKK39 pKa = 10.81RR40 pKa = 11.84KK41 pKa = 9.42

Molecular weight: 4.83 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

96

0

96

16926

34

1330

176.3

20.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.231 ± 0.404

0.786 ± 0.112

5.725 ± 0.208

9.134 ± 0.378

4.171 ± 0.231

6.251 ± 0.318

1.631 ± 0.122

6.239 ± 0.21

7.816 ± 0.268

8.271 ± 0.309

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.73 ± 0.146

5.175 ± 0.22

3.025 ± 0.196

3.326 ± 0.262

4.26 ± 0.216

6.014 ± 0.228

6.038 ± 0.305

6.54 ± 0.235

1.288 ± 0.116

4.348 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski