Dickeya phage Amaethon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Kafunavirus; unclassified Kafunavirus

Average proteome isoelectric point is 5.87

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GLJ3|A0A2S1GLJ3_9CAUD Uncharacterized protein OS=Dickeya phage Amaethon OX=2163629 PE=4 SV=1
MM1 pKa = 7.77IIGFVLVILSMTGQTAEE18 pKa = 4.27VVDD21 pKa = 3.82GDD23 pKa = 3.76IYY25 pKa = 11.42SLEE28 pKa = 3.98EE29 pKa = 4.04CQDD32 pKa = 3.26YY33 pKa = 8.25RR34 pKa = 11.84TSMEE38 pKa = 4.17SVHH41 pKa = 6.71PEE43 pKa = 3.73LHH45 pKa = 6.48FDD47 pKa = 3.82CAPVIRR53 pKa = 11.84DD54 pKa = 3.52VQII57 pKa = 3.71

Molecular weight: 6.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GLK6|A0A2S1GLK6_9CAUD Thymidylate synthase OS=Dickeya phage Amaethon OX=2163629 PE=3 SV=1
MM1 pKa = 7.42TNDD4 pKa = 4.59NIIQLWLSGAYY15 pKa = 8.76TEE17 pKa = 5.63SEE19 pKa = 4.12LCKK22 pKa = 10.37GLKK25 pKa = 9.34LSHH28 pKa = 5.42YY29 pKa = 10.26RR30 pKa = 11.84FRR32 pKa = 11.84KK33 pKa = 8.73LLKK36 pKa = 9.44VHH38 pKa = 7.07NIPAPPSSLTMPSLHH53 pKa = 6.34KK54 pKa = 10.2EE55 pKa = 4.08VYY57 pKa = 9.15NTVQHH62 pKa = 6.09TGRR65 pKa = 11.84PISQVAADD73 pKa = 4.49FGITIYY79 pKa = 10.26MATKK83 pKa = 10.11FATKK87 pKa = 10.63LEE89 pKa = 4.33TTKK92 pKa = 10.62EE93 pKa = 4.12VSQEE97 pKa = 3.98AIRR100 pKa = 11.84EE101 pKa = 4.17YY102 pKa = 10.48IVANDD107 pKa = 3.93ASPAEE112 pKa = 4.23LMAKK116 pKa = 10.18FNITRR121 pKa = 11.84YY122 pKa = 7.38TLNKK126 pKa = 9.57IAGDD130 pKa = 4.27LYY132 pKa = 10.62QPRR135 pKa = 11.84KK136 pKa = 10.02GHH138 pKa = 6.52IKK140 pKa = 9.49TDD142 pKa = 3.6SDD144 pKa = 3.91CTEE147 pKa = 3.85TQAKK151 pKa = 9.69VKK153 pKa = 10.2EE154 pKa = 4.03LLANGLSQTDD164 pKa = 3.46VAEE167 pKa = 4.38ALGISQSYY175 pKa = 8.86VSRR178 pKa = 11.84INPKK182 pKa = 9.47RR183 pKa = 11.84RR184 pKa = 11.84QHH186 pKa = 5.85NRR188 pKa = 11.84RR189 pKa = 11.84QALDD193 pKa = 3.54DD194 pKa = 3.97ATWEE198 pKa = 3.95QLKK201 pKa = 10.28SCRR204 pKa = 11.84HH205 pKa = 5.86HH206 pKa = 6.47YY207 pKa = 9.83SLRR210 pKa = 11.84QLASLFGVSKK220 pKa = 10.68SYY222 pKa = 10.41IIEE225 pKa = 4.03RR226 pKa = 11.84FKK228 pKa = 11.29RR229 pKa = 11.84EE230 pKa = 3.56AAII233 pKa = 4.07

Molecular weight: 26.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

12444

37

1010

254.0

28.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.225 ± 0.666

0.892 ± 0.139

6.356 ± 0.232

5.939 ± 0.249

3.431 ± 0.169

6.509 ± 0.37

1.551 ± 0.2

5.617 ± 0.249

6.164 ± 0.294

8.791 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.387 ± 0.179

5.264 ± 0.244

3.423 ± 0.191

4.42 ± 0.269

4.396 ± 0.256

6.622 ± 0.277

6.734 ± 0.347

6.638 ± 0.245

1.422 ± 0.174

4.219 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski