Daphnis nerii cypovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Cypovirus; unclassified Cypovirus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385LMP6|A0A385LMP6_9REOV Uncharacterized protein OS=Daphnis nerii cypovirus OX=1986950 PE=4 SV=1
MM1 pKa = 7.27MLYY4 pKa = 10.47AIDD7 pKa = 4.19VLNKK11 pKa = 8.54ATKK14 pKa = 9.68TIQQLRR20 pKa = 11.84VFKK23 pKa = 11.06VDD25 pKa = 3.35GQSTDD30 pKa = 3.77DD31 pKa = 3.34IRR33 pKa = 11.84ALRR36 pKa = 11.84TEE38 pKa = 4.44FEE40 pKa = 4.21RR41 pKa = 11.84LTGKK45 pKa = 10.5KK46 pKa = 9.84LLSRR50 pKa = 11.84KK51 pKa = 7.54YY52 pKa = 9.16TSALTSKK59 pKa = 10.73LFDD62 pKa = 3.47EE63 pKa = 5.23SVTKK67 pKa = 10.89NSNNKK72 pKa = 8.08LTYY75 pKa = 9.99HH76 pKa = 6.47IQNKK80 pKa = 9.73SMIGTHH86 pKa = 6.58LLSLDD91 pKa = 3.46TDD93 pKa = 3.92KK94 pKa = 11.65VIWTSAEE101 pKa = 4.1DD102 pKa = 3.49IKK104 pKa = 11.66SKK106 pKa = 9.93ICEE109 pKa = 4.28SEE111 pKa = 4.1VTLQSIKK118 pKa = 10.33MIEE121 pKa = 4.07SDD123 pKa = 3.69LTKK126 pKa = 10.57ALQMYY131 pKa = 10.51DD132 pKa = 3.23DD133 pKa = 4.11TAPTKK138 pKa = 10.01TVISADD144 pKa = 3.47EE145 pKa = 4.64RR146 pKa = 11.84SVSWKK151 pKa = 9.14TDD153 pKa = 2.83PKK155 pKa = 9.5MIKK158 pKa = 10.06VVIRR162 pKa = 11.84DD163 pKa = 3.97GKK165 pKa = 10.69DD166 pKa = 3.24EE167 pKa = 4.53SCFGLGTEE175 pKa = 5.16KK176 pKa = 10.52IPMRR180 pKa = 11.84DD181 pKa = 3.08SNFYY185 pKa = 10.67ANEE188 pKa = 3.76YY189 pKa = 10.66DD190 pKa = 4.62LEE192 pKa = 4.44QNMMASVNFLNVASSFDD209 pKa = 3.65DD210 pKa = 3.86SKK212 pKa = 11.75SLKK215 pKa = 10.43LKK217 pKa = 10.67SDD219 pKa = 3.62LAKK222 pKa = 10.89VGTAYY227 pKa = 9.46EE228 pKa = 4.36QWCNDD233 pKa = 3.1HH234 pKa = 6.82PQISDD239 pKa = 3.3QYY241 pKa = 7.33QVRR244 pKa = 11.84MIVRR248 pKa = 11.84ASTYY252 pKa = 10.17EE253 pKa = 3.77EE254 pKa = 4.34PLIKK258 pKa = 10.58DD259 pKa = 3.87LLSNIASTCIFTCAIYY275 pKa = 10.98NEE277 pKa = 4.68DD278 pKa = 3.86GTWSVTDD285 pKa = 4.08SFNDD289 pKa = 3.33TDD291 pKa = 3.9TVLAPVEE298 pKa = 4.24EE299 pKa = 4.81LSATANWSTPEE310 pKa = 4.11TVDD313 pKa = 3.59GQAEE317 pKa = 4.23QQVVASEE324 pKa = 4.77SIASATATTEE334 pKa = 4.33SNVDD338 pKa = 3.28TAINEE343 pKa = 4.05QAVEE347 pKa = 4.05INDD350 pKa = 3.4VDD352 pKa = 3.72EE353 pKa = 4.7STTEE357 pKa = 3.75ASQDD361 pKa = 2.96ATVIEE366 pKa = 4.33KK367 pKa = 10.31PLVEE371 pKa = 4.07EE372 pKa = 4.24EE373 pKa = 4.05QRR375 pKa = 11.84SVDD378 pKa = 3.38TTHH381 pKa = 7.95RR382 pKa = 11.84IQSVEE387 pKa = 3.91KK388 pKa = 9.38TVNAVPTKK396 pKa = 10.0PCEE399 pKa = 3.9KK400 pKa = 10.65DD401 pKa = 3.12EE402 pKa = 4.68LSNEE406 pKa = 3.85KK407 pKa = 8.97KK408 pKa = 9.24TLIRR412 pKa = 11.84LIGSEE417 pKa = 4.33LDD419 pKa = 3.19GDD421 pKa = 3.92KK422 pKa = 11.04PIVVNVASDD431 pKa = 3.6YY432 pKa = 10.54TLQDD436 pKa = 3.4QIEE439 pKa = 4.1DD440 pKa = 4.45LINNKK445 pKa = 7.49TANIRR450 pKa = 11.84IWHH453 pKa = 5.62NEE455 pKa = 3.2ILIGRR460 pKa = 11.84RR461 pKa = 11.84DD462 pKa = 3.93TIHH465 pKa = 6.9SLRR468 pKa = 11.84AGNLSEE474 pKa = 5.94FIIKK478 pKa = 9.64FQTITIPEE486 pKa = 4.44SNVPTFNVKK495 pKa = 10.53LNTGSNIGVEE505 pKa = 4.0PFAVKK510 pKa = 10.81NNTTRR515 pKa = 11.84SSNDD519 pKa = 3.05EE520 pKa = 3.86LFEE523 pKa = 4.39WGFKK527 pKa = 10.22TMVEE531 pKa = 4.28SLIKK535 pKa = 8.8THH537 pKa = 7.36LSFLHH542 pKa = 5.77STNPTADD549 pKa = 4.21RR550 pKa = 11.84IIITYY555 pKa = 8.3GKK557 pKa = 9.94KK558 pKa = 10.34YY559 pKa = 10.33KK560 pKa = 10.62ADD562 pKa = 3.83DD563 pKa = 4.11FEE565 pKa = 6.89LEE567 pKa = 4.2MVNGFRR573 pKa = 11.84VMTHH577 pKa = 6.14KK578 pKa = 10.42KK579 pKa = 10.31SKK581 pKa = 9.96ISAFKK586 pKa = 11.05SEE588 pKa = 4.43TIEE591 pKa = 4.43NLLKK595 pKa = 10.32MAGDD599 pKa = 4.45DD600 pKa = 3.51EE601 pKa = 5.19AKK603 pKa = 10.67RR604 pKa = 11.84NMIYY608 pKa = 10.73DD609 pKa = 3.85VIDD612 pKa = 4.73NIMSSCLSEE621 pKa = 5.04AEE623 pKa = 4.1SPYY626 pKa = 10.71TSFQQRR632 pKa = 11.84HH633 pKa = 5.35LNVIGLMRR641 pKa = 11.84DD642 pKa = 3.03

Molecular weight: 72.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385LMP0|A0A385LMP0_9REOV Uncharacterized protein OS=Daphnis nerii cypovirus OX=1986950 PE=4 SV=1
MM1 pKa = 7.7LFTINIRR8 pKa = 11.84APKK11 pKa = 9.14TLATLNRR18 pKa = 11.84MGIYY22 pKa = 10.24LRR24 pKa = 11.84EE25 pKa = 4.04QYY27 pKa = 10.64KK28 pKa = 10.42HH29 pKa = 6.13EE30 pKa = 4.28KK31 pKa = 8.72TSKK34 pKa = 10.77LLLKK38 pKa = 10.78RR39 pKa = 11.84NDD41 pKa = 4.13EE42 pKa = 4.02LHH44 pKa = 6.43HH45 pKa = 5.9WLEE48 pKa = 4.39KK49 pKa = 10.56LSARR53 pKa = 11.84HH54 pKa = 5.96KK55 pKa = 9.81GHH57 pKa = 6.14VFSMDD62 pKa = 2.66AVARR66 pKa = 11.84ILTEE70 pKa = 3.72RR71 pKa = 11.84LMKK74 pKa = 10.38INIIDD79 pKa = 3.57KK80 pKa = 10.71RR81 pKa = 11.84LGKK84 pKa = 10.74VKK86 pKa = 10.53DD87 pKa = 3.24IFANEE92 pKa = 4.38SKK94 pKa = 11.0NILQIVNKK102 pKa = 9.95EE103 pKa = 4.15DD104 pKa = 3.39VDD106 pKa = 4.47GVAMTYY112 pKa = 11.19DD113 pKa = 3.68DD114 pKa = 5.53ASFLLTLTKK123 pKa = 10.47EE124 pKa = 4.26VVIPNNEE131 pKa = 3.95TPVLYY136 pKa = 10.59EE137 pKa = 3.98IDD139 pKa = 4.19HH140 pKa = 6.12PTLRR144 pKa = 11.84HH145 pKa = 6.55LIPSYY150 pKa = 9.64IDD152 pKa = 3.04QMRR155 pKa = 11.84KK156 pKa = 8.42VLIIRR161 pKa = 11.84RR162 pKa = 11.84VTITDD167 pKa = 3.34VLYY170 pKa = 11.09DD171 pKa = 3.48NAISMRR177 pKa = 11.84NVFCQPRR184 pKa = 11.84IITTIMLEE192 pKa = 4.11KK193 pKa = 10.06EE194 pKa = 4.16VNEE197 pKa = 4.61AILWAKK203 pKa = 10.23RR204 pKa = 11.84KK205 pKa = 10.23RR206 pKa = 11.84EE207 pKa = 3.73VLYY210 pKa = 11.23VNNHH214 pKa = 5.99LYY216 pKa = 10.9KK217 pKa = 10.54LDD219 pKa = 3.38GLRR222 pKa = 11.84NQGVLAHH229 pKa = 6.62HH230 pKa = 7.05PKK232 pKa = 10.41ACEE235 pKa = 3.62ILLSGGQRR243 pKa = 11.84GCFEE247 pKa = 4.41VNLPSEE253 pKa = 4.46SLIHH257 pKa = 5.83AMKK260 pKa = 10.72RR261 pKa = 11.84EE262 pKa = 3.92LQTILNEE269 pKa = 4.16VIMFSVPPISYY280 pKa = 7.52DD281 pKa = 3.4TLLTGYY287 pKa = 10.31SKK289 pKa = 10.45ISRR292 pKa = 11.84IRR294 pKa = 11.84DD295 pKa = 3.34VPPIEE300 pKa = 4.11MRR302 pKa = 11.84LTALSALNRR311 pKa = 11.84LHH313 pKa = 7.77DD314 pKa = 4.06EE315 pKa = 4.14VTNTKK320 pKa = 8.7GTLFMTVYY328 pKa = 10.73ANKK331 pKa = 8.9GTGKK335 pKa = 7.53TTMTKK340 pKa = 10.02IMCEE344 pKa = 3.82KK345 pKa = 10.45LADD348 pKa = 3.4IYY350 pKa = 11.15KK351 pKa = 9.57RR352 pKa = 11.84PCYY355 pKa = 10.21RR356 pKa = 11.84IDD358 pKa = 3.3SDD360 pKa = 5.2AVGRR364 pKa = 11.84WLQTDD369 pKa = 3.3MSKK372 pKa = 10.49EE373 pKa = 3.95QFQKK377 pKa = 8.27MTFEE381 pKa = 5.08EE382 pKa = 3.93ILKK385 pKa = 10.06FNEE388 pKa = 4.12YY389 pKa = 8.69STSIYY394 pKa = 10.92EE395 pKa = 4.1VMAEE399 pKa = 4.13NIVAKK404 pKa = 10.72LGDD407 pKa = 3.5IKK409 pKa = 11.02LEE411 pKa = 3.92QILRR415 pKa = 11.84GRR417 pKa = 11.84YY418 pKa = 9.14SRR420 pKa = 11.84VNKK423 pKa = 10.27AIEE426 pKa = 3.81QFRR429 pKa = 11.84VEE431 pKa = 3.93RR432 pKa = 11.84GEE434 pKa = 4.36YY435 pKa = 10.04ILTTKK440 pKa = 10.5DD441 pKa = 3.34YY442 pKa = 9.91PAEE445 pKa = 3.66HH446 pKa = 6.63HH447 pKa = 6.97FYY449 pKa = 11.44SKK451 pKa = 10.22IASIAPYY458 pKa = 9.18GSIVIVEE465 pKa = 4.16GHH467 pKa = 6.03TLSQDD472 pKa = 2.95AYY474 pKa = 10.27LAPTNVSLIYY484 pKa = 10.21EE485 pKa = 4.67SIMDD489 pKa = 4.68PYY491 pKa = 11.21LAFNSRR497 pKa = 11.84DD498 pKa = 3.52NAKK501 pKa = 9.24TEE503 pKa = 3.92LFLYY507 pKa = 10.22LAYY510 pKa = 10.42DD511 pKa = 3.42RR512 pKa = 11.84EE513 pKa = 4.5LAHH516 pKa = 6.54SHH518 pKa = 5.94TKK520 pKa = 10.31FCMLEE525 pKa = 3.97FNDD528 pKa = 3.73WAVMRR533 pKa = 11.84SRR535 pKa = 11.84SNN537 pKa = 2.96

Molecular weight: 62.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

5623

300

1285

702.9

80.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.438 ± 0.491

0.96 ± 0.23

5.691 ± 0.317

6.971 ± 0.278

3.575 ± 0.204

4.659 ± 0.469

2.294 ± 0.233

7.452 ± 0.383

4.802 ± 0.488

9.319 ± 0.429

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.934 ± 0.211

6.384 ± 0.353

3.254 ± 0.204

3.379 ± 0.375

6.029 ± 0.32

7.505 ± 0.493

6.758 ± 0.398

6.367 ± 0.254

1.014 ± 0.125

4.215 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski