Prochlorococcus marinus (strain MIT 9211)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Prochloraceae; Prochlorococcus; Prochlorococcus marinus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1855 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A9BAA0|A9BAA0_PROM4 Uncharacterized protein OS=Prochlorococcus marinus (strain MIT 9211) OX=93059 GN=P9211_08311 PE=4 SV=1
MM1 pKa = 7.72KK2 pKa = 10.51LFQRR6 pKa = 11.84LLVAPAALGLMAPLAANAAEE26 pKa = 4.5VNIQDD31 pKa = 3.59VGNYY35 pKa = 10.06AGLEE39 pKa = 4.08EE40 pKa = 4.21QHH42 pKa = 6.69VEE44 pKa = 4.16TTAQFSDD51 pKa = 4.06VVPGDD56 pKa = 3.31WAYY59 pKa = 8.91TALQNLSEE67 pKa = 4.63SYY69 pKa = 10.7GCVGNAYY76 pKa = 10.01SQNLKK81 pKa = 10.37SGQALTRR88 pKa = 11.84YY89 pKa = 7.99EE90 pKa = 4.12AASLINSCLEE100 pKa = 4.13GGLVASGQGLSQDD113 pKa = 3.44AARR116 pKa = 11.84LSNEE120 pKa = 4.47FGSEE124 pKa = 3.69MAILKK129 pKa = 10.25GRR131 pKa = 11.84VDD133 pKa = 3.41GLEE136 pKa = 3.74YY137 pKa = 10.8RR138 pKa = 11.84LNEE141 pKa = 3.99VNAGTFANTTLISGSATFTVGAVDD165 pKa = 5.09DD166 pKa = 4.22STSGEE171 pKa = 4.2KK172 pKa = 10.21LHH174 pKa = 7.14SIYY177 pKa = 10.58AYY179 pKa = 10.03GIDD182 pKa = 3.54MSTSFSGEE190 pKa = 3.85DD191 pKa = 3.43SLDD194 pKa = 3.47VAVIAGNAGTQPLNVLDD211 pKa = 4.12SAEE214 pKa = 4.07GTTNQLQIDD223 pKa = 4.07KK224 pKa = 8.69LTYY227 pKa = 9.97SFPVGDD233 pKa = 4.2FRR235 pKa = 11.84VTAGPLMDD243 pKa = 3.94QDD245 pKa = 5.43DD246 pKa = 4.82IISAKK251 pKa = 10.15LSSYY255 pKa = 10.41SAEE258 pKa = 4.37FYY260 pKa = 8.95ITPHH264 pKa = 6.2EE265 pKa = 4.15YY266 pKa = 10.56SRR268 pKa = 11.84AEE270 pKa = 4.11TEE272 pKa = 3.91EE273 pKa = 4.74GPGVGATYY281 pKa = 10.37FFDD284 pKa = 4.29NKK286 pKa = 8.99WNASISAVFDD296 pKa = 4.78DD297 pKa = 6.24GDD299 pKa = 3.92DD300 pKa = 3.64ASNGILTRR308 pKa = 11.84EE309 pKa = 4.1GDD311 pKa = 3.92DD312 pKa = 4.26VITGSIGYY320 pKa = 9.92DD321 pKa = 3.12GDD323 pKa = 3.43GWGGGVILSSGDD335 pKa = 3.44KK336 pKa = 10.68NGSDD340 pKa = 3.38TTDD343 pKa = 3.24YY344 pKa = 11.59DD345 pKa = 3.39SWGWGLYY352 pKa = 8.75WEE354 pKa = 5.26PEE356 pKa = 3.91DD357 pKa = 4.93FPRR360 pKa = 11.84FSAGYY365 pKa = 7.65DD366 pKa = 3.32TQEE369 pKa = 4.23NEE371 pKa = 3.84GAADD375 pKa = 3.54NDD377 pKa = 3.27SWYY380 pKa = 10.14IAADD384 pKa = 3.55YY385 pKa = 10.76DD386 pKa = 3.69GWGPGTLSAAYY397 pKa = 9.36QVIDD401 pKa = 4.75DD402 pKa = 4.46EE403 pKa = 4.83TNEE406 pKa = 3.8LAAFEE411 pKa = 5.29VYY413 pKa = 10.78YY414 pKa = 10.89NWDD417 pKa = 3.43VADD420 pKa = 5.29GISVRR425 pKa = 11.84PGLFTQEE432 pKa = 3.96VAGGEE437 pKa = 4.3DD438 pKa = 3.12EE439 pKa = 4.34TGAVVEE445 pKa = 4.7TTFKK449 pKa = 10.93FF450 pKa = 3.57

Molecular weight: 47.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|A9BBJ0|RNPA_PROM4 Ribonuclease P protein component OS=Prochlorococcus marinus (strain MIT 9211) OX=93059 GN=rnpA PE=3 SV=1
MM1 pKa = 7.55TKK3 pKa = 9.01RR4 pKa = 11.84TLGGTSRR11 pKa = 11.84KK12 pKa = 9.07RR13 pKa = 11.84KK14 pKa = 8.17RR15 pKa = 11.84VSGFRR20 pKa = 11.84VRR22 pKa = 11.84MRR24 pKa = 11.84THH26 pKa = 5.95TGRR29 pKa = 11.84RR30 pKa = 11.84VIRR33 pKa = 11.84ARR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.38KK38 pKa = 10.52GRR40 pKa = 11.84SQLAVV45 pKa = 3.09

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1855

0

1855

505001

25

1531

272.2

30.31

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.16 ± 0.079

1.214 ± 0.021

4.966 ± 0.053

6.406 ± 0.064

3.987 ± 0.045

7.053 ± 0.053

1.843 ± 0.028

7.45 ± 0.058

6.305 ± 0.067

11.673 ± 0.084

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.978 ± 0.026

4.684 ± 0.042

4.372 ± 0.035

3.715 ± 0.036

5.105 ± 0.046

7.681 ± 0.042

4.685 ± 0.039

5.839 ± 0.05

1.472 ± 0.031

2.411 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski