Propionibacterium phage Pirate

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pahexavirus; Propionibacterium virus Pirate

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 44 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4IMU4|A0A0H4IMU4_9CAUD Helix-turn-helix DNA binding domain protein OS=Propionibacterium phage Pirate OX=1655018 GN=SEA_PIRATE_27 PE=4 SV=1
MM1 pKa = 7.48NNTHH5 pKa = 7.25NIPQAALKK13 pKa = 8.74TAVHH17 pKa = 6.82RR18 pKa = 11.84IIRR21 pKa = 11.84QQPTNMQQLEE31 pKa = 4.49NIVADD36 pKa = 3.38VEE38 pKa = 4.17NQYY41 pKa = 10.62RR42 pKa = 11.84IPISLDD48 pKa = 3.14NVNLTIKK55 pKa = 10.24EE56 pKa = 4.05VSLDD60 pKa = 3.58NLAINQDD67 pKa = 3.64TLDD70 pKa = 3.66EE71 pKa = 4.73CGEE74 pKa = 4.22ILWDD78 pKa = 4.35CDD80 pKa = 3.49SAGYY84 pKa = 6.93PTNNPQPEE92 pKa = 4.09ASQAALDD99 pKa = 3.84GLEE102 pKa = 4.02CLTYY106 pKa = 10.56KK107 pKa = 10.91ALTLITMVDD116 pKa = 3.76NILEE120 pKa = 4.85AIHH123 pKa = 6.43DD124 pKa = 3.95HH125 pKa = 6.7RR126 pKa = 11.84DD127 pKa = 3.31NYY129 pKa = 10.26PGIVKK134 pKa = 10.15QNIVDD139 pKa = 3.61QAKK142 pKa = 8.21YY143 pKa = 8.88TLAEE147 pKa = 4.37CALLHH152 pKa = 5.51QTLEE156 pKa = 5.37DD157 pKa = 3.69ILDD160 pKa = 4.26DD161 pKa = 4.06NLL163 pKa = 4.43

Molecular weight: 18.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4ISJ7|A0A0H4ISJ7_9CAUD Minor tail protein OS=Propionibacterium phage Pirate OX=1655018 GN=SEA_PIRATE_19 PE=4 SV=1
MM1 pKa = 7.85SGEE4 pKa = 3.79IASAYY9 pKa = 9.42VSLYY13 pKa = 10.73TKK15 pKa = 10.26MPGLKK20 pKa = 9.77SDD22 pKa = 3.48VGKK25 pKa = 10.2QLSGVMPAEE34 pKa = 4.46GQRR37 pKa = 11.84SGSLFAKK44 pKa = 10.08GMKK47 pKa = 9.56LALGGAAMVGAINVAKK63 pKa = 10.41KK64 pKa = 10.12GLKK67 pKa = 9.86SIYY70 pKa = 10.1DD71 pKa = 3.46VTIGGGIARR80 pKa = 11.84AMAIDD85 pKa = 3.74EE86 pKa = 4.58AQAKK90 pKa = 8.36LTGLGHH96 pKa = 6.51TSSDD100 pKa = 3.06TSSIMNSAIEE110 pKa = 4.15AVTGTSYY117 pKa = 11.95ALGDD121 pKa = 3.87AASTAAALSASGVKK135 pKa = 10.18SGGQMTDD142 pKa = 2.69VLKK145 pKa = 10.01TVADD149 pKa = 3.5VSYY152 pKa = 10.98ISGKK156 pKa = 9.96SFQDD160 pKa = 2.85TGAIFTSVMARR171 pKa = 11.84GKK173 pKa = 10.44LQGDD177 pKa = 4.11DD178 pKa = 3.62MLQLTMAGVPVLSLLARR195 pKa = 11.84QTGKK199 pKa = 9.04TSAEE203 pKa = 4.02VSQMVSKK210 pKa = 10.7GQIDD214 pKa = 4.08FATFSAAMKK223 pKa = 10.31LGMGGAAQASGKK235 pKa = 7.48TFEE238 pKa = 4.62GAMKK242 pKa = 10.13NVKK245 pKa = 9.81GALGYY250 pKa = 10.89LGATAMAPFLNGLRR264 pKa = 11.84QIFVALNPVIKK275 pKa = 10.57SVTDD279 pKa = 3.32SVKK282 pKa = 10.72PMFAAVDD289 pKa = 3.54AGIQRR294 pKa = 11.84MMPSILAWINRR305 pKa = 11.84MPGMITRR312 pKa = 11.84MNAQMRR318 pKa = 11.84AKK320 pKa = 10.19VEE322 pKa = 3.84QLKK325 pKa = 10.85SVFARR330 pKa = 11.84LHH332 pKa = 5.77LPVPKK337 pKa = 10.54VNLGAMFAGGTAVFGIVAAGVGKK360 pKa = 10.13LVAGFAPLAVSLKK373 pKa = 10.55NLLPSFGALKK383 pKa = 10.35GAAGGLGGVFRR394 pKa = 11.84ALGGPVGIVIGLFAAMFATNAQFRR418 pKa = 11.84AAVMQLVAVVGQALGQIMAAIQPLFGLVAGVVAQLAPVFGQIIGMVAGLAAQIVPLISMLVARR481 pKa = 11.84LVPVITQIIGAVTQVAAMLLPALMPVLQAVVAVIRR516 pKa = 11.84QVVGVVMQLVPVLVPVIQQILGAVMSVLPPIIGLIRR552 pKa = 11.84SLIPVIMSIMRR563 pKa = 11.84VVMQVVGVVLQVVARR578 pKa = 11.84IIPVVMPIVTAVIGFVARR596 pKa = 11.84ILGAVVSAAARR607 pKa = 11.84IIGTVTRR614 pKa = 11.84VIGWIVGHH622 pKa = 5.97FVSGVARR629 pKa = 11.84MGSVILNGWNHH640 pKa = 5.06IRR642 pKa = 11.84AFTSAFINGFKK653 pKa = 10.55SIISGGVNAVVGFFTRR669 pKa = 11.84LGLSVASHH677 pKa = 5.49VRR679 pKa = 11.84SGFNAARR686 pKa = 11.84GAVSSAMNAIRR697 pKa = 11.84SVVSSVTSAVGGFFSSMASRR717 pKa = 11.84VRR719 pKa = 11.84SGAVRR724 pKa = 11.84GFNGARR730 pKa = 11.84NAASSAMHH738 pKa = 6.4AMGSAVSSGVHH749 pKa = 5.07GVLGFFRR756 pKa = 11.84NLPGNIRR763 pKa = 11.84HH764 pKa = 6.5ALGNMGSLLVSAGRR778 pKa = 11.84DD779 pKa = 3.57VVSGLGNGIRR789 pKa = 11.84NAMSGLLDD797 pKa = 3.46TVRR800 pKa = 11.84NMGSQVANAAKK811 pKa = 10.09SVLGIHH817 pKa = 5.93SPSRR821 pKa = 11.84VFRR824 pKa = 11.84DD825 pKa = 3.43EE826 pKa = 3.91VGRR829 pKa = 11.84QVVAGLAEE837 pKa = 5.4GITGNAGLALDD848 pKa = 4.39AMSGVADD855 pKa = 4.76RR856 pKa = 11.84LPDD859 pKa = 3.5TVDD862 pKa = 2.54ARR864 pKa = 11.84FGVRR868 pKa = 11.84SSVGSFTPYY877 pKa = 10.31GRR879 pKa = 11.84YY880 pKa = 8.5QRR882 pKa = 11.84MSDD885 pKa = 2.89KK886 pKa = 11.06SVVVNVNGPTYY897 pKa = 10.88GDD899 pKa = 3.46PNEE902 pKa = 3.83FAKK905 pKa = 10.67RR906 pKa = 11.84IEE908 pKa = 4.16RR909 pKa = 11.84QQRR912 pKa = 11.84DD913 pKa = 3.73ALNALAYY920 pKa = 9.98VV921 pKa = 4.0

Molecular weight: 94.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

44

0

44

8877

27

921

201.8

22.1

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.756 ± 0.658

1.183 ± 0.208

6.534 ± 0.52

4.912 ± 0.444

2.771 ± 0.23

8.471 ± 0.634

2.591 ± 0.423

4.833 ± 0.358

3.988 ± 0.266

7.728 ± 0.265

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.49 ± 0.363

3.898 ± 0.373

5.035 ± 0.353

4.168 ± 0.326

6.117 ± 0.373

6.646 ± 0.402

6.263 ± 0.601

7.863 ± 0.826

2.028 ± 0.265

2.726 ± 0.296
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski