Amino acid dipepetide frequency for Tepidibacillus fermentans

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.706AlaAla: 4.706 ± 0.119
0.363AlaCys: 0.363 ± 0.024
2.865AlaAsp: 2.865 ± 0.072
4.171AlaGlu: 4.171 ± 0.091
2.579AlaPhe: 2.579 ± 0.059
4.645AlaGly: 4.645 ± 0.108
1.136AlaHis: 1.136 ± 0.044
6.485AlaIle: 6.485 ± 0.114
5.599AlaLys: 5.599 ± 0.092
6.267AlaLeu: 6.267 ± 0.114
1.884AlaMet: 1.884 ± 0.054
2.912AlaAsn: 2.912 ± 0.069
1.727AlaPro: 1.727 ± 0.053
2.088AlaGln: 2.088 ± 0.054
2.627AlaArg: 2.627 ± 0.068
3.138AlaSer: 3.138 ± 0.063
3.356AlaThr: 3.356 ± 0.069
4.364AlaVal: 4.364 ± 0.088
0.503AlaTrp: 0.503 ± 0.032
2.194AlaTyr: 2.194 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.238CysAla: 0.238 ± 0.018
0.053CysCys: 0.053 ± 0.009
0.243CysAsp: 0.243 ± 0.019
0.305CysGlu: 0.305 ± 0.021
0.185CysPhe: 0.185 ± 0.017
0.504CysGly: 0.504 ± 0.031
0.179CysHis: 0.179 ± 0.017
0.318CysIle: 0.318 ± 0.022
0.319CysLys: 0.319 ± 0.023
0.387CysLeu: 0.387 ± 0.025
0.114CysMet: 0.114 ± 0.013
0.239CysAsn: 0.239 ± 0.016
0.347CysPro: 0.347 ± 0.025
0.234CysGln: 0.234 ± 0.019
0.228CysArg: 0.228 ± 0.02
0.339CysSer: 0.339 ± 0.022
0.281CysThr: 0.281 ± 0.022
0.265CysVal: 0.265 ± 0.021
0.046CysTrp: 0.046 ± 0.008
0.182CysTyr: 0.182 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
2.657AspAla: 2.657 ± 0.082
0.221AspCys: 0.221 ± 0.018
2.21AspAsp: 2.21 ± 0.06
3.932AspGlu: 3.932 ± 0.073
2.378AspPhe: 2.378 ± 0.056
3.089AspGly: 3.089 ± 0.075
1.303AspHis: 1.303 ± 0.049
4.152AspIle: 4.152 ± 0.08
2.987AspLys: 2.987 ± 0.069
5.412AspLeu: 5.412 ± 0.089
1.145AspMet: 1.145 ± 0.039
1.442AspAsn: 1.442 ± 0.052
2.287AspPro: 2.287 ± 0.061
2.923AspGln: 2.923 ± 0.065
2.361AspArg: 2.361 ± 0.06
2.328AspSer: 2.328 ± 0.058
2.073AspThr: 2.073 ± 0.06
3.365AspVal: 3.365 ± 0.075
0.574AspTrp: 0.574 ± 0.034
2.127AspTyr: 2.127 ± 0.058
0.0AspXaa: 0.0 ± 0.0
Glu
4.616GluAla: 4.616 ± 0.1
0.236GluCys: 0.236 ± 0.02
3.352GluAsp: 3.352 ± 0.073
6.987GluGlu: 6.987 ± 0.124
2.52GluPhe: 2.52 ± 0.062
4.035GluGly: 4.035 ± 0.078
1.46GluHis: 1.46 ± 0.044
6.967GluIle: 6.967 ± 0.117
7.073GluLys: 7.073 ± 0.112
7.077GluLeu: 7.077 ± 0.095
2.458GluMet: 2.458 ± 0.066
3.554GluAsn: 3.554 ± 0.075
1.884GluPro: 1.884 ± 0.055
3.83GluGln: 3.83 ± 0.093
3.695GluArg: 3.695 ± 0.093
3.046GluSer: 3.046 ± 0.059
3.385GluThr: 3.385 ± 0.072
5.177GluVal: 5.177 ± 0.089
0.776GluTrp: 0.776 ± 0.036
2.414GluTyr: 2.414 ± 0.062
0.0GluXaa: 0.0 ± 0.0
Phe
2.805PheAla: 2.805 ± 0.073
0.232PheCys: 0.232 ± 0.018
2.332PheAsp: 2.332 ± 0.049
2.636PheGlu: 2.636 ± 0.065
2.261PhePhe: 2.261 ± 0.071
3.131PheGly: 3.131 ± 0.076
1.075PheHis: 1.075 ± 0.04
3.966PheIle: 3.966 ± 0.086
2.241PheLys: 2.241 ± 0.059
4.463PheLeu: 4.463 ± 0.111
1.111PheMet: 1.111 ± 0.041
1.656PheAsn: 1.656 ± 0.047
1.663PhePro: 1.663 ± 0.053
1.905PheGln: 1.905 ± 0.058
1.692PheArg: 1.692 ± 0.045
2.828PheSer: 2.828 ± 0.061
2.362PheThr: 2.362 ± 0.059
2.99PheVal: 2.99 ± 0.066
0.389PheTrp: 0.389 ± 0.028
1.665PheTyr: 1.665 ± 0.052
0.0PheXaa: 0.0 ± 0.0
Gly
4.342GlyAla: 4.342 ± 0.097
0.503GlyCys: 0.503 ± 0.031
3.047GlyAsp: 3.047 ± 0.072
4.229GlyGlu: 4.229 ± 0.081
3.284GlyPhe: 3.284 ± 0.084
4.601GlyGly: 4.601 ± 0.112
1.408GlyHis: 1.408 ± 0.049
6.99GlyIle: 6.99 ± 0.117
5.582GlyLys: 5.582 ± 0.086
6.644GlyLeu: 6.644 ± 0.136
2.036GlyMet: 2.036 ± 0.061
2.728GlyAsn: 2.728 ± 0.069
1.726GlyPro: 1.726 ± 0.068
2.325GlyGln: 2.325 ± 0.068
2.771GlyArg: 2.771 ± 0.065
3.524GlySer: 3.524 ± 0.074
3.919GlyThr: 3.919 ± 0.081
4.988GlyVal: 4.988 ± 0.095
0.743GlyTrp: 0.743 ± 0.035
2.848GlyTyr: 2.848 ± 0.068
0.0GlyXaa: 0.0 ± 0.0
His
1.227HisAla: 1.227 ± 0.042
0.178HisCys: 0.178 ± 0.016
0.951HisAsp: 0.951 ± 0.038
1.286HisGlu: 1.286 ± 0.04
1.116HisPhe: 1.116 ± 0.042
1.474HisGly: 1.474 ± 0.049
0.822HisHis: 0.822 ± 0.051
1.585HisIle: 1.585 ± 0.047
1.044HisLys: 1.044 ± 0.045
2.271HisLeu: 2.271 ± 0.061
0.477HisMet: 0.477 ± 0.025
0.725HisAsn: 0.725 ± 0.028
1.212HisPro: 1.212 ± 0.043
1.122HisGln: 1.122 ± 0.036
0.913HisArg: 0.913 ± 0.037
1.25HisSer: 1.25 ± 0.045
1.039HisThr: 1.039 ± 0.032
1.428HisVal: 1.428 ± 0.046
0.216HisTrp: 0.216 ± 0.02
0.891HisTyr: 0.891 ± 0.037
0.0HisXaa: 0.0 ± 0.0
Ile
6.344IleAla: 6.344 ± 0.096
0.441IleCys: 0.441 ± 0.029
5.056IleAsp: 5.056 ± 0.081
7.202IleGlu: 7.202 ± 0.104
3.665IlePhe: 3.665 ± 0.092
6.677IleGly: 6.677 ± 0.113
2.126IleHis: 2.126 ± 0.053
6.959IleIle: 6.959 ± 0.109
5.464IleLys: 5.464 ± 0.097
8.539IleLeu: 8.539 ± 0.128
1.89IleMet: 1.89 ± 0.059
3.286IleAsn: 3.286 ± 0.071
4.1IlePro: 4.1 ± 0.073
4.092IleGln: 4.092 ± 0.08
3.973IleArg: 3.973 ± 0.08
5.215IleSer: 5.215 ± 0.097
4.659IleThr: 4.659 ± 0.101
5.915IleVal: 5.915 ± 0.095
0.713IleTrp: 0.713 ± 0.038
2.969IleTyr: 2.969 ± 0.064
0.0IleXaa: 0.0 ± 0.0
Lys
4.702LysAla: 4.702 ± 0.088
0.272LysCys: 0.272 ± 0.02
4.01LysAsp: 4.01 ± 0.08
7.724LysGlu: 7.724 ± 0.131
1.964LysPhe: 1.964 ± 0.053
4.958LysGly: 4.958 ± 0.085
1.249LysHis: 1.249 ± 0.043
6.173LysIle: 6.173 ± 0.102
6.516LysLys: 6.516 ± 0.1
5.877LysLeu: 5.877 ± 0.102
2.375LysMet: 2.375 ± 0.057
3.736LysAsn: 3.736 ± 0.082
2.419LysPro: 2.419 ± 0.057
3.442LysGln: 3.442 ± 0.079
3.557LysArg: 3.557 ± 0.063
3.315LysSer: 3.315 ± 0.072
3.679LysThr: 3.679 ± 0.07
5.237LysVal: 5.237 ± 0.084
0.829LysTrp: 0.829 ± 0.042
2.449LysTyr: 2.449 ± 0.06
0.0LysXaa: 0.0 ± 0.0
Leu
6.973LeuAla: 6.973 ± 0.097
0.352LeuCys: 0.352 ± 0.027
4.981LeuAsp: 4.981 ± 0.091
6.826LeuGlu: 6.826 ± 0.121
4.588LeuPhe: 4.588 ± 0.097
6.925LeuGly: 6.925 ± 0.118
1.961LeuHis: 1.961 ± 0.059
7.973LeuIle: 7.973 ± 0.136
7.558LeuLys: 7.558 ± 0.109
9.538LeuLeu: 9.538 ± 0.18
2.438LeuMet: 2.438 ± 0.068
4.282LeuAsn: 4.282 ± 0.091
3.895LeuPro: 3.895 ± 0.072
3.869LeuGln: 3.869 ± 0.085
4.008LeuArg: 4.008 ± 0.079
6.214LeuSer: 6.214 ± 0.109
5.415LeuThr: 5.415 ± 0.086
6.114LeuVal: 6.114 ± 0.102
0.79LeuTrp: 0.79 ± 0.039
3.123LeuTyr: 3.123 ± 0.057
0.0LeuXaa: 0.0 ± 0.0
Met
2.059MetAla: 2.059 ± 0.063
0.093MetCys: 0.093 ± 0.012
1.415MetAsp: 1.415 ± 0.045
2.026MetGlu: 2.026 ± 0.055
0.921MetPhe: 0.921 ± 0.036
1.917MetGly: 1.917 ± 0.054
0.345MetHis: 0.345 ± 0.024
2.744MetIle: 2.744 ± 0.074
2.559MetLys: 2.559 ± 0.055
2.093MetLeu: 2.093 ± 0.061
0.943MetMet: 0.943 ± 0.043
1.662MetAsn: 1.662 ± 0.051
0.908MetPro: 0.908 ± 0.044
0.786MetGln: 0.786 ± 0.032
1.012MetArg: 1.012 ± 0.038
1.391MetSer: 1.391 ± 0.052
1.461MetThr: 1.461 ± 0.045
2.072MetVal: 2.072 ± 0.055
0.165MetTrp: 0.165 ± 0.015
0.664MetTyr: 0.664 ± 0.031
0.0MetXaa: 0.0 ± 0.0
Asn
2.503AsnAla: 2.503 ± 0.076
0.216AsnCys: 0.216 ± 0.02
1.995AsnAsp: 1.995 ± 0.063
3.319AsnGlu: 3.319 ± 0.076
1.559AsnPhe: 1.559 ± 0.047
3.155AsnGly: 3.155 ± 0.075
1.182AsnHis: 1.182 ± 0.04
3.516AsnIle: 3.516 ± 0.075
2.839AsnLys: 2.839 ± 0.07
4.204AsnLeu: 4.204 ± 0.073
1.178AsnMet: 1.178 ± 0.043
1.599AsnAsn: 1.599 ± 0.054
2.184AsnPro: 2.184 ± 0.06
2.865AsnGln: 2.865 ± 0.064
2.163AsnArg: 2.163 ± 0.055
2.096AsnSer: 2.096 ± 0.049
2.043AsnThr: 2.043 ± 0.059
2.913AsnVal: 2.913 ± 0.066
0.456AsnTrp: 0.456 ± 0.031
1.589AsnTyr: 1.589 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
1.915ProAla: 1.915 ± 0.052
0.169ProCys: 0.169 ± 0.013
1.897ProAsp: 1.897 ± 0.056
2.797ProGlu: 2.797 ± 0.059
1.902ProPhe: 1.902 ± 0.048
2.318ProGly: 2.318 ± 0.071
0.756ProHis: 0.756 ± 0.035
3.519ProIle: 3.519 ± 0.074
2.698ProLys: 2.698 ± 0.07
3.543ProLeu: 3.543 ± 0.076
0.863ProMet: 0.863 ± 0.038
1.966ProAsn: 1.966 ± 0.061
1.027ProPro: 1.027 ± 0.045
1.176ProGln: 1.176 ± 0.046
1.336ProArg: 1.336 ± 0.047
2.173ProSer: 2.173 ± 0.054
2.173ProThr: 2.173 ± 0.061
2.624ProVal: 2.624 ± 0.066
0.403ProTrp: 0.403 ± 0.028
1.559ProTyr: 1.559 ± 0.047
0.0ProXaa: 0.0 ± 0.0
Gln
2.807GlnAla: 2.807 ± 0.071
0.171GlnCys: 0.171 ± 0.018
1.719GlnAsp: 1.719 ± 0.055
3.194GlnGlu: 3.194 ± 0.072
1.868GlnPhe: 1.868 ± 0.056
2.444GlnGly: 2.444 ± 0.068
0.802GlnHis: 0.802 ± 0.036
4.102GlnIle: 4.102 ± 0.093
3.41GlnLys: 3.41 ± 0.083
4.809GlnLeu: 4.809 ± 0.105
1.354GlnMet: 1.354 ± 0.042
1.905GlnAsn: 1.905 ± 0.053
1.333GlnPro: 1.333 ± 0.044
2.191GlnGln: 2.191 ± 0.071
1.793GlnArg: 1.793 ± 0.052
2.209GlnSer: 2.209 ± 0.063
2.152GlnThr: 2.152 ± 0.059
2.854GlnVal: 2.854 ± 0.07
0.427GlnTrp: 0.427 ± 0.026
1.653GlnTyr: 1.653 ± 0.057
0.0GlnXaa: 0.0 ± 0.0
Arg
2.227ArgAla: 2.227 ± 0.063
0.211ArgCys: 0.211 ± 0.02
2.019ArgAsp: 2.019 ± 0.053
3.464ArgGlu: 3.464 ± 0.087
2.139ArgPhe: 2.139 ± 0.054
2.576ArgGly: 2.576 ± 0.069
0.743ArgHis: 0.743 ± 0.027
3.924ArgIle: 3.924 ± 0.081
3.611ArgLys: 3.611 ± 0.084
4.51ArgLeu: 4.51 ± 0.082
1.37ArgMet: 1.37 ± 0.043
2.008ArgAsn: 2.008 ± 0.053
1.467ArgPro: 1.467 ± 0.043
1.672ArgGln: 1.672 ± 0.043
1.914ArgArg: 1.914 ± 0.058
2.304ArgSer: 2.304 ± 0.055
1.992ArgThr: 1.992 ± 0.056
2.835ArgVal: 2.835 ± 0.066
0.406ArgTrp: 0.406 ± 0.025
1.667ArgTyr: 1.667 ± 0.054
0.0ArgXaa: 0.0 ± 0.0
Ser
3.013SerAla: 3.013 ± 0.085
0.295SerCys: 0.295 ± 0.023
2.314SerAsp: 2.314 ± 0.063
3.39SerGlu: 3.39 ± 0.074
2.821SerPhe: 2.821 ± 0.076
3.806SerGly: 3.806 ± 0.075
1.054SerHis: 1.054 ± 0.036
5.132SerIle: 5.132 ± 0.093
3.65SerLys: 3.65 ± 0.066
5.885SerLeu: 5.885 ± 0.1
1.586SerMet: 1.586 ± 0.049
2.409SerAsn: 2.409 ± 0.067
2.018SerPro: 2.018 ± 0.057
2.105SerGln: 2.105 ± 0.059
2.224SerArg: 2.224 ± 0.067
3.312SerSer: 3.312 ± 0.111
2.963SerThr: 2.963 ± 0.073
3.47SerVal: 3.47 ± 0.068
0.486SerTrp: 0.486 ± 0.027
2.058SerTyr: 2.058 ± 0.063
0.0SerXaa: 0.0 ± 0.0
Thr
3.366ThrAla: 3.366 ± 0.076
0.315ThrCys: 0.315 ± 0.024
2.354ThrAsp: 2.354 ± 0.054
3.252ThrGlu: 3.252 ± 0.066
2.394ThrPhe: 2.394 ± 0.055
4.097ThrGly: 4.097 ± 0.089
1.021ThrHis: 1.021 ± 0.039
5.046ThrIle: 5.046 ± 0.085
3.658ThrLys: 3.658 ± 0.073
5.021ThrLeu: 5.021 ± 0.083
1.249ThrMet: 1.249 ± 0.041
2.496ThrAsn: 2.496 ± 0.067
2.237ThrPro: 2.237 ± 0.054
1.528ThrGln: 1.528 ± 0.049
1.858ThrArg: 1.858 ± 0.045
2.882ThrSer: 2.882 ± 0.069
2.911ThrThr: 2.911 ± 0.084
3.722ThrVal: 3.722 ± 0.079
0.48ThrTrp: 0.48 ± 0.027
1.9ThrTyr: 1.9 ± 0.065
0.0ThrXaa: 0.0 ± 0.0
Val
4.729ValAla: 4.729 ± 0.089
0.423ValCys: 0.423 ± 0.026
3.712ValAsp: 3.712 ± 0.072
4.847ValGlu: 4.847 ± 0.094
2.928ValPhe: 2.928 ± 0.057
4.745ValGly: 4.745 ± 0.1
1.417ValHis: 1.417 ± 0.044
6.238ValIle: 6.238 ± 0.084
4.935ValLys: 4.935 ± 0.095
6.331ValLeu: 6.331 ± 0.091
1.733ValMet: 1.733 ± 0.053
3.076ValAsn: 3.076 ± 0.07
2.536ValPro: 2.536 ± 0.061
2.406ValGln: 2.406 ± 0.059
2.718ValArg: 2.718 ± 0.065
3.974ValSer: 3.974 ± 0.075
3.798ValThr: 3.798 ± 0.087
4.97ValVal: 4.97 ± 0.096
0.551ValTrp: 0.551 ± 0.03
2.228ValTyr: 2.228 ± 0.055
0.0ValXaa: 0.0 ± 0.0
Trp
0.486TrpAla: 0.486 ± 0.029
0.041TrpCys: 0.041 ± 0.008
0.461TrpAsp: 0.461 ± 0.028
0.617TrpGlu: 0.617 ± 0.033
0.523TrpPhe: 0.523 ± 0.033
0.57TrpGly: 0.57 ± 0.03
0.155TrpHis: 0.155 ± 0.015
0.931TrpIle: 0.931 ± 0.041
0.768TrpLys: 0.768 ± 0.03
1.075TrpLeu: 1.075 ± 0.049
0.299TrpMet: 0.299 ± 0.025
0.533TrpAsn: 0.533 ± 0.027
0.228TrpPro: 0.228 ± 0.019
0.36TrpGln: 0.36 ± 0.024
0.38TrpArg: 0.38 ± 0.022
0.424TrpSer: 0.424 ± 0.029
0.433TrpThr: 0.433 ± 0.026
0.685TrpVal: 0.685 ± 0.034
0.122TrpTrp: 0.122 ± 0.014
0.312TrpTyr: 0.312 ± 0.026
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.895TyrAla: 1.895 ± 0.059
0.221TyrCys: 0.221 ± 0.019
2.032TyrAsp: 2.032 ± 0.086
2.34TyrGlu: 2.34 ± 0.067
1.808TyrPhe: 1.808 ± 0.052
2.573TyrGly: 2.573 ± 0.064
1.035TyrHis: 1.035 ± 0.041
2.566TyrIle: 2.566 ± 0.062
1.971TyrLys: 1.971 ± 0.06
3.877TyrLeu: 3.877 ± 0.086
0.733TyrMet: 0.733 ± 0.033
1.36TyrAsn: 1.36 ± 0.05
1.618TyrPro: 1.618 ± 0.046
2.275TyrGln: 2.275 ± 0.054
1.835TyrArg: 1.835 ± 0.054
2.028TyrSer: 2.028 ± 0.061
1.692TyrThr: 1.692 ± 0.057
2.305TyrVal: 2.305 ± 0.056
0.328TyrTrp: 0.328 ± 0.023
1.564TyrTyr: 1.564 ± 0.055
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2415 proteins (702271 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski