Escherichia phage PGN590

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Drexlerviridae; Tempevirinae; Hanrivervirus; Escherichia virus PGN590

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 51 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M9EFT5|A0A6M9EFT5_9CAUD Uncharacterized protein OS=Escherichia phage PGN590 OX=2714735 PE=4 SV=1
MM1 pKa = 7.74SDD3 pKa = 3.19YY4 pKa = 11.28SVIVGVEE11 pKa = 4.11SPGCMVDD18 pKa = 3.27SSIMVINGSFVCGDD32 pKa = 3.41EE33 pKa = 5.08FIAPGKK39 pKa = 9.8IVRR42 pKa = 11.84LSHH45 pKa = 5.55ITNGYY50 pKa = 9.37KK51 pKa = 9.7IASINGDD58 pKa = 3.77LVIGVAVRR66 pKa = 11.84PLDD69 pKa = 3.42SCIYY73 pKa = 10.38EE74 pKa = 4.58DD75 pKa = 4.57GDD77 pKa = 4.33PISVVSRR84 pKa = 11.84GIVWCLTSEE93 pKa = 4.37SEE95 pKa = 4.24APEE98 pKa = 4.21YY99 pKa = 10.36GDD101 pKa = 4.61KK102 pKa = 11.37VFVTQDD108 pKa = 2.18GDD110 pKa = 3.43AAYY113 pKa = 10.76SEE115 pKa = 4.8GDD117 pKa = 4.26LLSGWIFTGEE127 pKa = 4.12HH128 pKa = 5.33VKK130 pKa = 10.26VDD132 pKa = 3.4HH133 pKa = 7.37DD134 pKa = 4.49SYY136 pKa = 11.47IVGVSIDD143 pKa = 3.38SS144 pKa = 3.47

Molecular weight: 15.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M9ED77|A0A6M9ED77_9CAUD Uncharacterized protein OS=Escherichia phage PGN590 OX=2714735 PE=4 SV=1
MM1 pKa = 7.36FSSPIARR8 pKa = 11.84KK9 pKa = 7.57TSSLMIVSLRR19 pKa = 11.84LSSLRR24 pKa = 11.84RR25 pKa = 11.84MINASYY31 pKa = 11.04LMPLSLSHH39 pKa = 6.31LQVSAITSSLRR50 pKa = 11.84PVRR53 pKa = 11.84ITMANPDD60 pKa = 3.13IHH62 pKa = 6.81NPLTTATVLLIGEE75 pKa = 4.58SPTAALLNSDD85 pKa = 3.99QLSVRR90 pKa = 11.84CKK92 pKa = 9.43SSVGSRR98 pKa = 11.84CKK100 pKa = 10.39RR101 pKa = 11.84CVPSDD106 pKa = 3.68KK107 pKa = 10.69VPSRR111 pKa = 11.84STITIGLLVEE121 pKa = 4.27KK122 pKa = 9.7WYY124 pKa = 10.74LLPQSSNSYY133 pKa = 10.3FFIIKK138 pKa = 9.64PINN141 pKa = 3.22

Molecular weight: 15.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

51

0

51

11847

101

890

232.3

25.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.357 ± 0.48

1.469 ± 0.196

5.622 ± 0.338

6.322 ± 0.256

3.942 ± 0.194

7.183 ± 0.308

1.849 ± 0.21

6.871 ± 0.254

7.31 ± 0.315

6.896 ± 0.256

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.887 ± 0.186

4.752 ± 0.219

3.697 ± 0.243

3.942 ± 0.314

5.25 ± 0.31

6.651 ± 0.363

5.52 ± 0.235

6.525 ± 0.223

1.367 ± 0.132

3.587 ± 0.171
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski