Eremothecium cymbalariae (strain CBS 270.75 / DBVPG 7215 / KCTC 17166 / NRRL Y-17582) (Yeast)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Eremothecium; Eremothecium cymbalariae

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4429 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8JU12|G8JU12_ERECY CAP-Gly domain-containing protein OS=Eremothecium cymbalariae (strain CBS 270.75 / DBVPG 7215 / KCTC 17166 / NRRL Y-17582) OX=931890 GN=Ecym_4475 PE=4 SV=1
MM1 pKa = 7.46RR2 pKa = 11.84TTNAVAATAALLAVASAEE20 pKa = 4.04DD21 pKa = 4.06LEE23 pKa = 4.45PAQVSSIDD31 pKa = 4.23VVVQDD36 pKa = 4.25IKK38 pKa = 11.51SHH40 pKa = 4.45LTEE43 pKa = 4.02YY44 pKa = 10.89VSYY47 pKa = 10.77AGSNSGLSIPEE58 pKa = 4.09DD59 pKa = 3.65VISVYY64 pKa = 8.91TQVATYY70 pKa = 10.14TDD72 pKa = 3.52GSYY75 pKa = 10.97TSLFSNLNFQEE86 pKa = 4.01INSAITGLPWYY97 pKa = 10.04SSRR100 pKa = 11.84LAPAFASLSPAAATDD115 pKa = 3.98APQSSEE121 pKa = 4.11GSDD124 pKa = 3.34SSNSGSGVVPAPAPVPVGGGSGSSDD149 pKa = 3.18SSSSSGSSGSEE160 pKa = 3.7SGSSSEE166 pKa = 4.36SASGSDD172 pKa = 3.49SAGGAAAAASSSEE185 pKa = 4.02GSSSGSSSSASATSSATHH203 pKa = 6.35SNASRR208 pKa = 11.84NNSSTSNSRR217 pKa = 11.84AGANNLQVLSTGLGIAAAGIVGLLLL242 pKa = 4.03

Molecular weight: 23.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8JX58|G8JX58_ERECY SPX domain-containing protein OS=Eremothecium cymbalariae (strain CBS 270.75 / DBVPG 7215 / KCTC 17166 / NRRL Y-17582) OX=931890 GN=Ecym_8141 PE=4 SV=1
MM1 pKa = 7.1RR2 pKa = 11.84AKK4 pKa = 9.23WKK6 pKa = 9.85KK7 pKa = 9.54KK8 pKa = 8.19RR9 pKa = 11.84VRR11 pKa = 11.84RR12 pKa = 11.84LKK14 pKa = 9.71RR15 pKa = 11.84KK16 pKa = 8.27RR17 pKa = 11.84RR18 pKa = 11.84KK19 pKa = 9.19VRR21 pKa = 11.84ARR23 pKa = 11.84SKK25 pKa = 10.98

Molecular weight: 3.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4429

0

4429

2154228

25

4915

486.4

54.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.788 ± 0.033

1.344 ± 0.013

5.757 ± 0.029

6.513 ± 0.032

4.219 ± 0.027

5.508 ± 0.035

2.269 ± 0.016

6.158 ± 0.024

6.742 ± 0.039

9.778 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.155 ± 0.013

5.27 ± 0.024

4.385 ± 0.03

4.239 ± 0.034

4.802 ± 0.02

8.823 ± 0.048

5.542 ± 0.028

6.203 ± 0.024

1.075 ± 0.011

3.431 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski