Opuntia virus X

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Alphaflexiviridae; Potexvirus

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6UNH9|Q6UNH9_9VIRU Triple gene block protein 2 OS=Opuntia virus X OX=253702 PE=4 SV=1
MM1 pKa = 7.44LVLSFLVGAALGLALLAALHH21 pKa = 5.78TGEE24 pKa = 5.3NKK26 pKa = 10.39GCLIQFNGHH35 pKa = 4.64STIVSNCQDD44 pKa = 3.69VKK46 pKa = 11.47DD47 pKa = 3.78LAAVINAINDD57 pKa = 3.69RR58 pKa = 11.84LSFSNN63 pKa = 3.76

Molecular weight: 6.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6UNI0|Q6UNI0_9VIRU Triple gene block protein 1 OS=Opuntia virus X OX=253702 PE=4 SV=1
MM1 pKa = 7.51SGHH4 pKa = 7.14PLRR7 pKa = 11.84LTPPPDD13 pKa = 3.18YY14 pKa = 11.02SKK16 pKa = 11.38AVTSLTIGLTLVLAVYY32 pKa = 10.22AVTRR36 pKa = 11.84STLPTVGDD44 pKa = 3.93SSHH47 pKa = 6.53SLPHH51 pKa = 6.08GGFYY55 pKa = 10.49KK56 pKa = 10.84DD57 pKa = 3.12GTKK60 pKa = 8.77TVKK63 pKa = 10.78YY64 pKa = 10.13LGPSDD69 pKa = 3.78NHH71 pKa = 6.6SKK73 pKa = 10.62HH74 pKa = 6.02IPLLAVLVIIALIYY88 pKa = 10.62ASSFLSSGRR97 pKa = 11.84RR98 pKa = 11.84TGTCSSCGTAHH109 pKa = 7.32GG110 pKa = 4.16

Molecular weight: 11.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

2186

63

1555

437.2

48.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.143 ± 1.03

1.51 ± 0.42

4.895 ± 0.586

5.672 ± 1.236

4.941 ± 0.501

4.986 ± 0.995

3.339 ± 0.519

5.672 ± 0.381

6.496 ± 1.235

9.515 ± 1.147

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.013 ± 0.405

4.209 ± 1.048

6.176 ± 0.695

4.895 ± 1.061

3.843 ± 0.314

7.182 ± 1.575

7.274 ± 0.715

5.078 ± 0.618

1.281 ± 0.433

2.882 ± 0.633
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski