Enterococcus phage vB_EfaS_HEf13

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Saphexavirus; unclassified Saphexavirus

Average proteome isoelectric point is 5.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A510C2N0|A0A510C2N0_9CAUD Uncharacterized protein OS=Enterococcus phage vB_EfaS_HEf13 OX=2283014 GN=vBEfaSHEf13_035 PE=4 SV=1
MM1 pKa = 7.91IEE3 pKa = 4.22LFKK6 pKa = 11.43EE7 pKa = 3.93NLTLDD12 pKa = 3.65EE13 pKa = 5.02MSEE16 pKa = 3.58IWYY19 pKa = 9.15GWDD22 pKa = 4.23NEE24 pKa = 3.9GLLYY28 pKa = 10.6GWEE31 pKa = 3.94NGEE34 pKa = 4.07AGEE37 pKa = 4.79AYY39 pKa = 10.4ALLYY43 pKa = 10.29EE44 pKa = 4.64NGYY47 pKa = 11.14NMDD50 pKa = 4.3SIKK53 pKa = 10.61VLHH56 pKa = 6.42EE57 pKa = 4.26AFDD60 pKa = 4.49TIGEE64 pKa = 4.39LIGAII69 pKa = 3.92

Molecular weight: 7.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A510C2U9|A0A510C2U9_9CAUD Terminase large subunit OS=Enterococcus phage vB_EfaS_HEf13 OX=2283014 GN=vBEfaSHEf13_069 PE=4 SV=1
MM1 pKa = 7.86PYY3 pKa = 10.24KK4 pKa = 10.78GLFKK8 pKa = 10.62HH9 pKa = 6.77FYY11 pKa = 10.4AFLKK15 pKa = 10.87AFLALFTRR23 pKa = 11.84LFARR27 pKa = 11.84SLLKK31 pKa = 10.54SIKK34 pKa = 9.53TLLL37 pKa = 3.77

Molecular weight: 4.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

16763

37

1330

176.5

20.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.242 ± 0.505

0.698 ± 0.089

6.312 ± 0.217

9.181 ± 0.414

4.259 ± 0.237

6.091 ± 0.369

1.456 ± 0.116

6.264 ± 0.229

7.779 ± 0.301

8.596 ± 0.377

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.164

5.023 ± 0.187

2.959 ± 0.227

3.496 ± 0.286

4.027 ± 0.217

5.906 ± 0.222

6.001 ± 0.307

6.485 ± 0.23

1.312 ± 0.117

4.23 ± 0.245
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski