Chloracidobacterium thermophilum (strain B)

Taxonomy: cellular organisms; Bacteria; Acidobacteria; Blastocatellia; Chloracidobacterium; Chloracidobacterium thermophilum

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3053 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G2LI80|G2LI80_CHLTF Putative GTPase OS=Chloracidobacterium thermophilum (strain B) OX=981222 GN=Cabther_A0781 PE=4 SV=1
MM1 pKa = 7.53QDD3 pKa = 2.81TATYY7 pKa = 7.81TCAYY11 pKa = 9.3CGEE14 pKa = 4.47PNVTFPDD21 pKa = 3.25WSGGRR26 pKa = 11.84RR27 pKa = 11.84QQYY30 pKa = 10.1IEE32 pKa = 4.9DD33 pKa = 4.14CTVCCQPNVLYY44 pKa = 9.58VTLEE48 pKa = 3.93PDD50 pKa = 3.5GEE52 pKa = 4.83TVCVFAEE59 pKa = 4.25AGG61 pKa = 3.4

Molecular weight: 6.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G2LDG9|G2LDG9_CHLTF Uncharacterized protein OS=Chloracidobacterium thermophilum (strain B) OX=981222 GN=Cabther_A1682 PE=4 SV=1
MM1 pKa = 7.55PADD4 pKa = 3.7GQRR7 pKa = 11.84ARR9 pKa = 11.84GRR11 pKa = 11.84EE12 pKa = 3.85TLRR15 pKa = 11.84GRR17 pKa = 11.84RR18 pKa = 11.84CAACGYY24 pKa = 9.94RR25 pKa = 11.84IGGFHH30 pKa = 7.21WKK32 pKa = 8.49TFGLGVCLGLSLGAGQVVWQAVQPPPPLPPLTTAQALTGSPPVGPEE78 pKa = 3.74TSRR81 pKa = 11.84EE82 pKa = 4.1TAGTTLSRR90 pKa = 11.84EE91 pKa = 4.02RR92 pKa = 11.84HH93 pKa = 5.16RR94 pKa = 11.84CGAPTKK100 pKa = 10.47KK101 pKa = 9.41GTPCQRR107 pKa = 11.84WVSGATGYY115 pKa = 10.07CWQHH119 pKa = 6.49RR120 pKa = 11.84KK121 pKa = 9.59DD122 pKa = 3.39

Molecular weight: 13.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3053

0

3053

1097942

17

2522

359.6

39.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.37 ± 0.058

1.01 ± 0.014

4.94 ± 0.027

6.022 ± 0.052

3.9 ± 0.035

7.672 ± 0.052

2.091 ± 0.024

4.541 ± 0.029

2.849 ± 0.034

10.817 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.827 ± 0.018

2.694 ± 0.039

5.947 ± 0.044

3.872 ± 0.027

7.963 ± 0.04

4.759 ± 0.027

6.085 ± 0.038

7.639 ± 0.035

1.405 ± 0.023

2.599 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski