Lactococcus phage P078

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Nevevirus; Lactococcus virus P078

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X4Y7K4|X4Y7K4_9CAUD Uncharacterized protein OS=Lactococcus phage P078 OX=1476886 GN=P078_0033 PE=4 SV=1
MM1 pKa = 7.45IKK3 pKa = 10.34AITMDD8 pKa = 3.78SEE10 pKa = 4.16QLRR13 pKa = 11.84NLLYY17 pKa = 10.18IEE19 pKa = 4.41YY20 pKa = 9.1PEE22 pKa = 4.54SNIDD26 pKa = 3.74EE27 pKa = 4.63IEE29 pKa = 4.27VLDD32 pKa = 3.97TVPEE36 pKa = 4.26EE37 pKa = 3.94EE38 pKa = 4.54AEE40 pKa = 4.45TVVRR44 pKa = 11.84EE45 pKa = 3.88LLVNYY50 pKa = 8.18TEE52 pKa = 5.5HH53 pKa = 7.18GINYY57 pKa = 9.1NDD59 pKa = 3.73TVDD62 pKa = 4.71IIVDD66 pKa = 3.61DD67 pKa = 4.15EE68 pKa = 4.71PYY70 pKa = 11.32YY71 pKa = 11.29NDD73 pKa = 3.41IPMKK77 pKa = 10.89VLFDD81 pKa = 3.6YY82 pKa = 10.53MKK84 pKa = 10.62DD85 pKa = 3.36YY86 pKa = 11.48GKK88 pKa = 8.39TCFSIILKK96 pKa = 8.88LTEE99 pKa = 4.17EE100 pKa = 4.86YY101 pKa = 11.09YY102 pKa = 11.19SDD104 pKa = 4.95FLPLDD109 pKa = 3.93TAQSANDD116 pKa = 3.33ITKK119 pKa = 9.24TLIKK123 pKa = 10.62

Molecular weight: 14.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X4YGG6|X4YGG6_9CAUD Uncharacterized protein OS=Lactococcus phage P078 OX=1476886 GN=P078_0012 PE=4 SV=1
MM1 pKa = 8.42DD2 pKa = 4.33YY3 pKa = 9.42NTYY6 pKa = 10.72QDD8 pKa = 3.53ILLHH12 pKa = 5.77YY13 pKa = 9.79GVIGMKK19 pKa = 6.97WHH21 pKa = 6.72KK22 pKa = 10.23VRR24 pKa = 11.84ARR26 pKa = 11.84AAEE29 pKa = 3.95KK30 pKa = 10.58KK31 pKa = 10.05AVKK34 pKa = 9.78KK35 pKa = 10.15AQKK38 pKa = 9.46EE39 pKa = 4.21YY40 pKa = 10.88QKK42 pKa = 11.07GWQNQYY48 pKa = 11.49ANRR51 pKa = 11.84HH52 pKa = 5.46KK53 pKa = 10.74MSDD56 pKa = 3.28DD57 pKa = 3.48QMRR60 pKa = 11.84KK61 pKa = 9.29AVNRR65 pKa = 11.84LQMEE69 pKa = 4.82SNFARR74 pKa = 11.84LTAEE78 pKa = 3.98TTISEE83 pKa = 4.6RR84 pKa = 11.84KK85 pKa = 8.94RR86 pKa = 11.84AAAYY90 pKa = 7.26TRR92 pKa = 11.84QFKK95 pKa = 9.45STTVKK100 pKa = 10.91DD101 pKa = 3.66MNDD104 pKa = 3.03SFKK107 pKa = 11.25EE108 pKa = 3.98NEE110 pKa = 4.13KK111 pKa = 10.43LRR113 pKa = 11.84KK114 pKa = 9.23NVTRR118 pKa = 11.84AIAAVVVV125 pKa = 3.77

Molecular weight: 14.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

16970

38

1692

217.6

24.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.594 ± 0.603

0.554 ± 0.105

6.818 ± 0.3

6.199 ± 0.472

3.724 ± 0.202

6.394 ± 0.503

1.632 ± 0.178

7.472 ± 0.297

8.067 ± 0.434

7.938 ± 0.333

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.876 ± 0.186

6.235 ± 0.344

3.306 ± 0.197

3.324 ± 0.15

3.718 ± 0.256

6.464 ± 0.306

6.735 ± 0.329

6.376 ± 0.199

1.196 ± 0.117

4.378 ± 0.455
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski