Thalassotalea sp. PP2-459

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales; Colwelliaceae; Thalassotalea; unclassified Thalassotalea

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3513 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q6CFF7|A0A1Q6CFF7_9GAMM Uncharacterized protein OS=Thalassotalea sp. PP2-459 OX=1742724 GN=BI291_03890 PE=4 SV=1
MM1 pKa = 7.5SNIKK5 pKa = 9.41TKK7 pKa = 7.61TRR9 pKa = 11.84KK10 pKa = 9.73LISAMSLASMAVMGQANADD29 pKa = 4.31LLSLDD34 pKa = 4.07IDD36 pKa = 3.79NSNAQAYY43 pKa = 7.88IASGNGDD50 pKa = 3.32NSVTRR55 pKa = 11.84GDD57 pKa = 3.67NLASITDD64 pKa = 4.16PNTASSPFSGVVSIFIEE81 pKa = 4.31MNGSGFICTGAALNKK96 pKa = 10.04RR97 pKa = 11.84HH98 pKa = 6.35ILSAAHH104 pKa = 6.7CVDD107 pKa = 4.42EE108 pKa = 4.73NDD110 pKa = 3.63QGTTINLADD119 pKa = 4.16PNNNVRR125 pKa = 11.84VIFNSDD131 pKa = 2.55GTYY134 pKa = 10.95NDD136 pKa = 4.54IITAQKK142 pKa = 10.5VDD144 pKa = 3.22IHH146 pKa = 6.61PGYY149 pKa = 10.74DD150 pKa = 3.41GFNVCPDD157 pKa = 3.4GSIGCVNDD165 pKa = 3.52DD166 pKa = 3.49VAIIEE171 pKa = 4.55LDD173 pKa = 3.25RR174 pKa = 11.84DD175 pKa = 3.4IPAGTKK181 pKa = 9.71TYY183 pKa = 11.06DD184 pKa = 3.39LFSGQVFDD192 pKa = 4.69TNNLANQGGGDD203 pKa = 3.51GSLFTMVGYY212 pKa = 7.56GTRR215 pKa = 11.84GDD217 pKa = 4.3GYY219 pKa = 10.92SGYY222 pKa = 8.66YY223 pKa = 9.44TNDD226 pKa = 2.95GDD228 pKa = 5.53ADD230 pKa = 3.84GSPDD234 pKa = 3.69FFSKK238 pKa = 10.58LVGMNIVDD246 pKa = 5.62AISFDD251 pKa = 4.5DD252 pKa = 4.77DD253 pKa = 4.16GSGQAEE259 pKa = 4.27VWHH262 pKa = 6.95ADD264 pKa = 3.02FDD266 pKa = 4.24GTYY269 pKa = 10.15TDD271 pKa = 4.39NDD273 pKa = 3.89DD274 pKa = 4.54LFGGGAGEE282 pKa = 4.59EE283 pKa = 4.14YY284 pKa = 10.83DD285 pKa = 4.96FDD287 pKa = 4.55FFCDD291 pKa = 3.5VYY293 pKa = 10.67GICSSWLPEE302 pKa = 4.44GIEE305 pKa = 4.03ANIGGGDD312 pKa = 3.23SGGPSFVYY320 pKa = 10.6NSLTDD325 pKa = 3.33TYY327 pKa = 10.2EE328 pKa = 3.62IAGINTFGARR338 pKa = 11.84TSPVAPGAYY347 pKa = 8.93GDD349 pKa = 3.85RR350 pKa = 11.84FGGILMNPYY359 pKa = 10.22ANWIAAQVSQIPTPGSIMLFGLALAGLATARR390 pKa = 11.84RR391 pKa = 11.84KK392 pKa = 9.72AA393 pKa = 3.23

Molecular weight: 41.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q6CMF1|A0A1Q6CMF1_9GAMM 50S ribosomal protein L17 OS=Thalassotalea sp. PP2-459 OX=1742724 GN=rplQ PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.3NGRR28 pKa = 11.84AVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.39GRR39 pKa = 11.84ARR41 pKa = 11.84LSAA44 pKa = 3.91

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3513

0

3513

1188626

38

2227

338.4

37.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.177 ± 0.037

0.96 ± 0.014

5.449 ± 0.036

6.073 ± 0.038

4.377 ± 0.027

6.206 ± 0.041

2.332 ± 0.02

7.092 ± 0.036

5.904 ± 0.041

10.184 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.354 ± 0.018

5.006 ± 0.037

3.59 ± 0.021

4.833 ± 0.041

3.988 ± 0.026

6.719 ± 0.036

5.699 ± 0.028

6.643 ± 0.035

1.16 ± 0.014

3.256 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski