Mesonia sp. K7

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Mesonia; unclassified Mesonia

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2375 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2W1K6Y6|A0A2W1K6Y6_9FLAO DUF192 domain-containing protein OS=Mesonia sp. K7 OX=2218606 GN=DNG35_06485 PE=4 SV=1
MM1 pKa = 7.5KK2 pKa = 10.33KK3 pKa = 10.44LLFFYY8 pKa = 10.56FLLIGFYY15 pKa = 10.11TYY17 pKa = 11.0GQVGIGTAMPDD28 pKa = 3.27PSAQLDD34 pKa = 3.82VTVAAGDD41 pKa = 3.65KK42 pKa = 10.74GVLLPRR48 pKa = 11.84VALINTTDD56 pKa = 3.02QTTITNGNVEE66 pKa = 4.3SLLVYY71 pKa = 9.2NTATQNNVTPGYY83 pKa = 8.59YY84 pKa = 9.55YY85 pKa = 9.88WYY87 pKa = 8.5NGSWRR92 pKa = 11.84RR93 pKa = 11.84LTALGDD99 pKa = 3.69HH100 pKa = 7.42PEE102 pKa = 4.51TITTLVNNTDD112 pKa = 2.62GSYY115 pKa = 10.05TYY117 pKa = 10.1TSEE120 pKa = 4.68DD121 pKa = 2.97GTMTTFTVGGMMVDD135 pKa = 3.82NGDD138 pKa = 3.26GTYY141 pKa = 10.64TFTNPDD147 pKa = 3.3GTNVTIQGVSQALTILSYY165 pKa = 11.63DD166 pKa = 3.71NNTGMISYY174 pKa = 9.76IDD176 pKa = 3.87EE177 pKa = 4.55NSTLTQLDD185 pKa = 3.49MSLAIDD191 pKa = 3.27SWEE194 pKa = 4.0TLTDD198 pKa = 4.08LKK200 pKa = 11.2LNPDD204 pKa = 4.81NIHH207 pKa = 7.18LDD209 pKa = 3.6YY210 pKa = 10.88TDD212 pKa = 3.77EE213 pKa = 5.83DD214 pKa = 4.67GVTNQIDD221 pKa = 4.3LTTLVQNLEE230 pKa = 4.11TLTTIVANADD240 pKa = 3.3GTFTYY245 pKa = 10.53TDD247 pKa = 3.44EE248 pKa = 4.83NGNATQIDD256 pKa = 3.9ISNLEE261 pKa = 4.23TLTSLALNADD271 pKa = 4.08GKK273 pKa = 8.0TLEE276 pKa = 4.29YY277 pKa = 9.66TDD279 pKa = 4.0EE280 pKa = 4.75SGTVTTVDD288 pKa = 3.67LSSVITNFEE297 pKa = 4.54TITTLVDD304 pKa = 3.59NGDD307 pKa = 3.23GTYY310 pKa = 10.07TYY312 pKa = 10.47TNEE315 pKa = 4.26SGNTTVIDD323 pKa = 3.88VNNTEE328 pKa = 4.29TLTEE332 pKa = 4.26LVLSTDD338 pKa = 3.41NSTLTYY344 pKa = 10.04TDD346 pKa = 3.61EE347 pKa = 4.64NGNDD351 pKa = 3.63TIINLKK357 pKa = 9.07TVVQANQKK365 pKa = 6.03TTTLADD371 pKa = 3.76GVNTTVSSTVSGDD384 pKa = 3.1NTAWQVNVATAKK396 pKa = 10.34GANNTQNSTLGVVKK410 pKa = 10.15EE411 pKa = 4.14ADD413 pKa = 3.58SNPTVNIANDD423 pKa = 3.66GSLSVNLEE431 pKa = 3.62NTNDD435 pKa = 3.3IKK437 pKa = 10.96EE438 pKa = 4.04VSGNYY443 pKa = 8.2TVLPNDD449 pKa = 4.2TILLGNASTADD460 pKa = 3.53VSIVMPNPVGKK471 pKa = 9.99KK472 pKa = 10.26GKK474 pKa = 8.71TITIKK479 pKa = 10.81KK480 pKa = 9.56QDD482 pKa = 3.8TNEE485 pKa = 3.75DD486 pKa = 4.14YY487 pKa = 11.3YY488 pKa = 10.83INVYY492 pKa = 10.98GNINGLSQLYY502 pKa = 7.91TALPYY507 pKa = 10.78SGWQLTSDD515 pKa = 3.84GTQWKK520 pKa = 7.93ITNKK524 pKa = 9.86FF525 pKa = 3.15

Molecular weight: 56.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2W1KG00|A0A2W1KG00_9FLAO Uncharacterized protein OS=Mesonia sp. K7 OX=2218606 GN=DNG35_05730 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.01RR21 pKa = 11.84MATVNGRR28 pKa = 11.84KK29 pKa = 9.18VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2375

0

2375

780029

38

2477

328.4

37.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.245 ± 0.049

0.72 ± 0.015

5.429 ± 0.04

7.275 ± 0.058

5.234 ± 0.046

6.076 ± 0.055

1.819 ± 0.024

8.0 ± 0.048

8.027 ± 0.09

9.16 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.126 ± 0.026

6.214 ± 0.056

3.333 ± 0.035

3.874 ± 0.033

3.295 ± 0.032

6.104 ± 0.042

5.726 ± 0.074

6.194 ± 0.036

0.961 ± 0.02

4.189 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski